Abstract
The present study envisaged the physiological and molecular characterization of ASR gene of pigeon pea genotypes under drought stress condition. All the 12 pigeon pea genotypes were subjected to physiological characterization under drought stress condition for identification of ASR gene in the field in three replications under control, 06 days, and 12 days conditions during summer 2023-24. The data from summer 2023-24 was subjected to statistical analysis. The Analysis of variance (ANOVA) for drought stress tolerance or resistance index was performed for all 12 genotypes using RBD analysis software. ANOVA showed non-significant genotypic differences for drought stress tolerance or resistance index and also between replication there was no significant difference for all 12 pigeon pea genotypes shown in the result of the RBD analysis. There was an unknowingly less is more in all the parameters measured from day 0–12 of drought stress in all the genotypes with control. Control plants maintained a higher protein content (leaf) (A), Fresh shoot weight (B), Fresh weight (C), Turgid weight (D), Dry weight (E), and Shoot dry matter content (F) in comparison to drought stressed plants. In the current study, the analysis of physiological responses demonstrated that ASR gene expression in drought stress and in control condition neither improve not reduced in water stress, which is helped to maintain studied physiological characters under water stress. A perusal of dendrogram indicated that an elucidian distance of 0.16 indicating that pigeon pea genotypes did not show much variation at DNA level and out of total 1775 amplicons polymorphism detected only 25.77% polymorphism with 9.61 average numbers of polymorphic bands. The designed primer(s) are perfect but not binding with the target and giving single sharp band on a DNA electrophoresis gel. It can be concluded that the parameters used for primer designing by Primer3 play a critical role in the experimental results and parameters defined in the present study resulting in a negative amplification during PCR of all 12 genotypes. Therefore, it was concluded that the 12 pigeon pea genotypes collected from ARS, Badnapur don’t show the amplification of ASR gene. According to the comparative analysis results of all genotypes belongs to drought susceptible.
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Claims 11
Claim · model-written summary
Genotypes did not differ significantly in drought tolerance index.
- Taxon
- pigeon pea
Cajanus cajant0 - Outcome
- drought stress tolerance or resistance indexe0
- Direction
- non-significante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“ANOVA showed non-significant genotypic differences for drought stress tolerance or resistance index”
Claim · model-written summary
Replications did not differ significantly in drought tolerance index.
- Taxon
- pigeon peaCajanus cajant0
- Outcome
- no significant differencee0
- Direction
- no significant differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“also between replication there was no significant difference for all 12 pigeon pea genotypes”
Claim · model-written summary
Control plants had higher leaf protein content than drought-stressed plants.
- Taxon
- pigeon peaCajanus cajant0
- Treatment
- drought stressed plantse0
- Outcome
- protein content (leaf)e0
- Direction
- highere0
- Comparator
- Control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“Control plants maintained a higher protein content (leaf) (A), Fresh shoot weight (B), Fresh weight (C), Turgid weight (D), Dry weight (E), and Shoot dry matter content (F) in comparison to drought stressed plants”
Claim · model-written summary
Control plants had higher fresh shoot weight, fresh weight and turgid weight than drought-stressed plants.
- Taxon
- pigeon peaCajanus cajant0
- Treatment
- drought stressed plantse0
- Outcome
- Fresh shoot weight (B), Fresh weight (C), Turgid weight (D)e0
- Direction
- highere0
- Comparator
- Control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“Control plants maintained a higher protein content (leaf) (A), Fresh shoot weight (B), Fresh weight (C), Turgid weight (D), Dry weight (E), and Shoot dry matter content (F) in comparison to drought stressed plants”
Claim · model-written summary
Control plants had higher dry weight and shoot dry matter than drought-stressed plants.
- Taxon
- pigeon peaCajanus cajant0
- Treatment
- drought stressed plantse0
- Outcome
- Dry weight (E), and Shoot dry matter content (F)e0
- Direction
- highere0
- Comparator
- Control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“Control plants maintained a higher protein content (leaf) (A), Fresh shoot weight (B), Fresh weight (C), Turgid weight (D), Dry weight (E), and Shoot dry matter content (F) in comparison to drought stressed plants”
Claim · model-written summary
ASR expression reportedly neither increased nor decreased under drought stress.
- Taxon
- pigeon peaCajanus cajant0
- Gene
- ASR genee0
- Outcome
- ASR gene expressione0
- Direction
- neither improve not reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“ASR gene expression in drought stress and in control condition neither improve not reduced in water stress”
Claim · model-written summary
The dendrogram showed little DNA-level variation among pigeon pea genotypes, at distance 0.16.
- Taxon
- pigeon peaCajanus cajant0
- Outcome
- variation at DNA levele0
- Value
- 0.16e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“an elucidian distance of 0.16 indicating that pigeon pea genotypes did not show much variation at DNA level”
Claim · model-written summary
Of 1775 amplicons, 25.77% were polymorphic.
- Taxon
- pigeon peaCajanus cajant0
- Outcome
- polymorphisme0
- Value
- 25.77%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“out of total 1775 amplicons polymorphism detected only 25.77% polymorphism”
Claim · model-written summary
The mean number of polymorphic bands was 9.61.
- Taxon
- pigeon peaCajanus cajant0
- Outcome
- average numbers of polymorphic bandse0
- Value
- 9.61e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“9.61 average numbers of polymorphic bands”
Claim · model-written summary
Designed primers did not bind the target and ASR failed to amplify in all 12 genotypes.
- Taxon
- pigeon peaCajanus cajant0
- Gene
- ASR genee0
- Outcome
- amplification of ASR genee0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“the 12 pigeon pea genotypes collected from ARS, Badnapur don’t show the amplification of ASR gene”
Claim · model-written summary
All genotypes were classified as drought susceptible.
- Taxon
- pigeon peaCajanus cajant0
- Outcome
- drought susceptiblee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pigeon pea (Cajanus cajan (L.) Millsp.)”
e0“all genotypes belongs to drought susceptible”
The same questions in other papers
drought · quality: protein · Cajanus cajan (pigeon pea)Positive and negative results
1 in the opposite direction
- positive
drought · growth · Cajanus cajan (pigeon pea)Positive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.