Abstract
Background: Hamelia patens Jacq. (HP) is widely recognized in traditional medicine for its antimicrobial properties, which are attributed to secondary metabolites such as phenolic compounds, alkaloids, and terpenes. Fusarium oxysporum f. sp. radicis-lycopersici (Fo), a phytopathogenic fungus affecting economically important crops, is managed with fungicides like benzimidazoles and azoles. Excessive use of these compounds has led to resistance and environmental contamination, highlighting the need for sustainable alternatives. This study aimed to optimize the extraction of secondary metabolites from HP leaves and flowers, evaluate their antifungal activity, and assess the impact of extraction methods and plant parts on chemical composition and efficacy. Methods: Three extraction methods were employed: consecutive maceration (CM) using solvents of ascending polarity; total maceration (TM), which is a single-step methanol-based method; and ultrasound-assisted maceration (UAM) employing ultrasonic waves with methanol. Extracts were characterized by quantifying total phenols (TP), condensed tannins (TC), flavonoids (Fl), alkaloids (TA), sterols (TS), and saponins (S) using colorimetric assays and UPLC-MS. Multivariate analyses, including PCA, PLS-DA, OPLS-DA, and Pearson correlation, evaluated the relationships between the chemical profiles and antifungal activity. Results: Leaf extracts exhibited higher flavonoid and tannin contents than flower extracts. CMML showed the highest antifungal activity (IC50 3.7% w/v), which was associated with elevated levels of these compounds. Significant correlations linked antifungal activity with rutin (HP21) and kaempferol-3-O-β-rutinoside (HP29). Conclusions: Methanolic extracts of HP exhibited significant antifungal activity against Fo. These findings highlight the importance of optimizing extraction methods and selecting specific plant parts to enhance bioactive compound efficacy, offering a sustainable approach to pathogen management.
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Claims 6
Claim · model-written summary
Hamelia patens leaf extracts had higher flavonoid and tannin contents than flower extracts.
- Taxon
- Hamelia patens Jacq.
Hamelia patenst0 - Organ / stage
- Leafe0
- Outcome
- flavonoid and tannin contentse0
- Direction
- highere0
- Comparator
- flower extractse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hamelia patens Jacq.”
e0“Leaf extracts exhibited higher flavonoid and tannin contents than flower extracts”
Claim · model-written summary
The CMML extract showed the highest antifungal activity against Fusarium oxysporum f. sp. radicis-lycopersici (IC50 3.7% w/v).
- Taxon
- Hamelia patens Jacq.Hamelia patenst0
- Material
- CMMLe0
- Interactor
- Fusarium oxysporum f. sp. radicis-lycopersici
Fusarium oxysporum f. sp. radicis-lycopersicit1pathogen - Outcome
- antifungal activitye0
- Direction
- higheste0
- Value
- 3.7% w/ve0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hamelia patens Jacq.”
t1“Fusarium oxysporum f. sp. radicis-lycopersici”
e0“CMML showed the highest antifungal activity (IC50 3.7% w/v)”
Claim · model-written summary
The highest antifungal activity of CMML was associated with elevated levels of flavonoids and tannins.
- Taxon
- Hamelia patens Jacq.Hamelia patenst0
- Compound
- flavonoid and tannine0
- Outcome
- antifungal activitye0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hamelia patens Jacq.”
e0“Leaf extracts exhibited higher flavonoid and tannin contents than flower extracts. CMML showed the highest antifungal activity (IC50 3.7% w/v), which was associated with elevated levels of these compounds”
Claim · model-written summary
Significant correlations linked antifungal activity with rutin (HP21) and kaempferol-3-O-beta-rutinoside (HP29).
- Taxon
- Hamelia patens Jacq.Hamelia patenst0
- Compound
- rutin (HP21) and kaempferol-3-O-β-rutinoside (HP29)e0
- Outcome
- antifungal activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hamelia patens Jacq.”
e0“Significant correlations linked antifungal activity with rutin (HP21) and kaempferol-3-O-β-rutinoside (HP29)”
Claim · model-written summary
Methanolic extracts of Hamelia patens exhibited significant antifungal activity against Fusarium oxysporum f. sp. radicis-lycopersici.
Not reportedComparatorDirection
Verbatim
e0“Methanolic extracts of HP exhibited significant antifungal activity against Fo”
Claim · model-written summary
Optimizing extraction methods and selecting specific plant parts is important to enhance bioactive compound efficacy.
- Taxon
- Hamelia patens Jacq.Hamelia patenst0
- Outcome
- bioactive compound efficacye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hamelia patens Jacq.”
e0“the importance of optimizing extraction methods and selecting specific plant parts to enhance bioactive compound efficacy”