Paper · 2025 · International Journal of Molecular Sciences

Impact of Water Stress on Metabolic Intermediates and Regulators in Broccoli Sprouts, and Cellular Defense Potential of Their Extracts

Ivana Šola; Daria Gmižić; Karlo Miškec; et al.

20
claims
40
verbatim quotes
10
citations
1
research areas
✓
Open access · cc-by

Abstract

Duch.) sprouts. Drought and flood differently influenced chlorophylls, carotenoids, and porphyrins, with drought having a stronger inhibitory effect on chlorophyll a, total chlorophyll, and porphyrins. Carotenoids and glucosinolates increased under drought but decreased with flooding, suggesting that these compounds play a crucial role in drought tolerance. Nitrate increased with drought from 13.11 ± 1.05 mg/g dw to 22.41 ± 1.20 mg/g dw but decreased under flooding to 5.17 ± 1.03 mg/g dw, and oxalic acid was reduced by drought only (from 48.94 ± 1.30 mg/g dw to 46.43 ± 0.64 mg/g dw). Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%, while drought decreased flavonoids by 23%. Total phenolics and proanthocyanidins were increased by drought by 29% and 7%, respectively, while flooding decreased hydroxycinnamic acids by 13%. Both stress types influenced individual polyphenols differently: drought diminished ferulic acid by 17% and increased sinapic acid by 30%, while flooding reversed these effects and enhanced kaempferol by 22%. These compounds, along with proline (which increased by 139% under drought), emerged as biomarkers of water stress. Flood impacted antioxidant capacity more significantly, while drought-stressed broccoli extracts better protected plasmid DNA against oxidative damage. These findings underline the metabolic plasticity of broccoli sprouts and their potential in targeted crop management for water stress resilience.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 20

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Drought had a stronger inhibitory effect than flood on chlorophyll a, total chlorophyll and porphyrins in broccoli sprouts.

Treatment
droughte1
Outcome
chlorophyll a, total chlorophyll, and porphyrinse1
Direction
stronger inhibitory effecte1
Comparator
floode1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Drought and flood differently influenced chlorophylls, carotenoids, and porphyrins, with drought having a stronger inhibitory effect on chlorophyll a, total chlorophyll, and porphyrins”

✓ located verbatim in the source · abstract, characters 16–200 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Carotenoids and glucosinolates increased under drought but decreased with flooding in broccoli sprouts.

Treatment
droughte1
Outcome
Carotenoids and glucosinolatese1
Direction
increasede1
Qualifier
but decreased with floodinge1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Carotenoids and glucosinolates increased under drought but decreased with flooding”

✓ located verbatim in the source · abstract, characters 202–284 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Nitrate content increased under drought in broccoli sprouts.

Treatment
droughte1
Outcome
Nitratee1
Direction
increasede1
Value
from 13.11 ± 1.05 mg/g dw to 22.41 ± 1.20 mg/g dwe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Nitrate increased with drought from 13.11 ± 1.05 mg/g dw to 22.41 ± 1.20 mg/g dw but decreased under flooding to 5.17 ± 1.03 mg/g dw”

✓ located verbatim in the source · abstract, characters 360–492 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Nitrate content decreased under flooding in broccoli sprouts.

Treatment
floodinge1
Outcome
Nitratee1
Direction
decreasede1
Value
5.17 ± 1.03 mg/g dwe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Nitrate increased with drought from 13.11 ± 1.05 mg/g dw to 22.41 ± 1.20 mg/g dw but decreased under flooding to 5.17 ± 1.03 mg/g dw”

✓ located verbatim in the source · abstract, characters 360–492 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Oxalic acid was reduced by drought only in broccoli sprouts.

Treatment
droughte1
Outcome
oxalic acide1
Direction
reducede1
Value
from 48.94 ± 1.30 mg/g dw to 46.43 ± 0.64 mg/g dwe1
Qualifier
drought onlye1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“oxalic acid was reduced by drought only (from 48.94 ± 1.30 mg/g dw to 46.43 ± 0.64 mg/g dw)”

✓ located verbatim in the source · abstract, characters 498–589 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flood reduced protein content by 29% in broccoli sprouts.

Treatment
Floode1
Outcome
proteinse1
Direction
reducede1
Value
29%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%”

✓ located verbatim in the source · abstract, characters 591–721 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flood reduced phenolics by 15% in broccoli sprouts.

Treatment
Floode1
Outcome
phenolicse1
Direction
reducede1
Value
15%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%”

✓ located verbatim in the source · abstract, characters 591–721 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flood reduced flavonoids by 10% in broccoli sprouts.

Treatment
Floode1
Outcome
flavonoidse1
Direction
reducede1
Value
10%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%”

✓ located verbatim in the source · abstract, characters 591–721 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flood reduced flavonols by 11% in broccoli sprouts.

Treatment
Floode1
Outcome
flavonolse1
Direction
reducede1
Value
11%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%”

✓ located verbatim in the source · abstract, characters 591–721 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flood reduced tannins by 36% in broccoli sprouts.

Treatment
Floode1
Outcome
tanninse1
Direction
reducede1
Value
36%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%”

✓ located verbatim in the source · abstract, characters 591–721 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flood reduced proanthocyanidins by 19% in broccoli sprouts.

Treatment
Floode1
Outcome
proanthocyanidinse1
Direction
reducede1
Value
19%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood reduced proteins by 29%, phenolics by 15%, flavonoids by 10%, flavonols by 11%, tannins by 36%, and proanthocyanidins by 19%”

✓ located verbatim in the source · abstract, characters 591–721 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Drought decreased flavonoids by 23% in broccoli sprouts.

Treatment
droughte1
Outcome
flavonoidse1
Direction
decreasede1
Value
23%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“while drought decreased flavonoids by 23%”

✓ located verbatim in the source · abstract, characters 723–764 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Drought increased total phenolics by 29% in broccoli sprouts.

Treatment
droughte1
Outcome
Total phenolicse1
Direction
increasede1
Value
29%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Total phenolics and proanthocyanidins were increased by drought by 29% and 7%, respectively”

✓ located verbatim in the source · abstract, characters 766–857 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Drought increased proanthocyanidins by 7% in broccoli sprouts.

Treatment
droughte1
Outcome
proanthocyanidinse1
Direction
increasede1
Value
7%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Total phenolics and proanthocyanidins were increased by drought by 29% and 7%, respectively”

✓ located verbatim in the source · abstract, characters 766–857 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flooding decreased hydroxycinnamic acids by 13% in broccoli sprouts.

Treatment
floodinge1
Outcome
hydroxycinnamic acidse1
Direction
decreasede1
Value
13%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“while flooding decreased hydroxycinnamic acids by 13%”

✓ located verbatim in the source · abstract, characters 859–912 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Drought diminished ferulic acid by 17% in broccoli sprouts.

Treatment
droughte1
Outcome
ferulic acide1
Direction
diminishede1
Value
17%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“drought diminished ferulic acid by 17% and increased sinapic acid by 30%”

✓ located verbatim in the source · abstract, characters 979–1051 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Drought increased sinapic acid by 30% in broccoli sprouts.

Treatment
droughte1
Outcome
sinapic acide1
Direction
increasede1
Value
30%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“drought diminished ferulic acid by 17% and increased sinapic acid by 30%”

✓ located verbatim in the source · abstract, characters 979–1051 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Flooding enhanced kaempferol by 22% in broccoli sprouts.

Treatment
floodinge1
Outcome
kaempferole1
Direction
enhancede1
Value
22%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“flooding reversed these effects and enhanced kaempferol by 22%”

✓ located verbatim in the source · abstract, characters 1059–1121 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Drought increased proline by 139% in broccoli sprouts.

Treatment
droughte1
Outcome
prolinee1
Direction
increasede1
Value
139%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“proline (which increased by 139% under drought)”

✓ located verbatim in the source · abstract, characters 1151–1198 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Flood impacted antioxidant capacity more significantly, and drought-stressed broccoli extracts better protected plasmid DNA against oxidative damage.

Outcome
antioxidant capacitye1
Qualifier
better protected plasmid DNA against oxidative damagee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Broccoli Sprouts”

✓ located verbatim in the source · title, characters 68–84 · show in text ↑
e1

“Flood impacted antioxidant capacity more significantly, while drought-stressed broccoli extracts better protected plasmid DNA against oxidative damage”

✓ located verbatim in the source · abstract, characters 1239–1389 · show in text ↑

drought · physiology: pigments · Brassica oleracea (wild cabbage)Results differ

7 papers: 1 positive, 6 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. negative

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.