Paper · 2025 · Polish Journal of Environmental Studies

Enhancing Wheat Growth: Impact ofPGPR Co-Inoculation with Azospirillumlipoferum and Agrobacterium fabrum

Muhammad Aown Sammar Raza; Ahsan Saleem; Imran Haider Khan; et al.

9
claims
22
verbatim quotes
2
citations
3
research areas
✓
Open access

Abstract

The significance of co-inoculation of plant growth-promoting rhizobacteria (PGPR) in crop development is understudied. A wirehouse experiment in Pakistan examined how PGPR-inoculated wheat seedlings affected growth and yield. The experimental design included four treatments: T0 (control), T1 (Azospirillum lipoferum), T2 (Agrobacterium fabrum), and T3 (co-inoculation). This study examined development, growth, and wheat yield. Co-inoculation increased wheat grain output by 36%, grains per plant by 11%, and 1000-grain weight by 17% compared to the non-inoculated reference. Crop growth increased by 6.3% during tillering and 37% at flowering. T3 outperformed T1 and T2 inoculations by 9% and 14%, respectively. Compared to the control treatment, co-inoculation increased leaf epicuticular wax and relative water content. In essence, inoculating wheat seeds with A. lipoferum and A. fabrum separately and together may improve wheat growth, yield, and quality. This research provides essential information for improving agricultural methods to preserve and increase crop output.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased wheat grain output by 36% compared with the non-inoculated reference.

Treatment
Co-inoculatione1
Outcome
grain outpute1
Direction
increasede1
Value
36%e1
Comparator
non-inoculated referencee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e1

“Co-inoculation increased wheat grain output by 36%, grains per plant by 11%, and 1000-grain weight by 17% compared to the non-inoculated reference”

✓ located verbatim in the source · abstract, characters 429–575 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased wheat grains per plant by 11% compared with the non-inoculated reference.

Treatment
Co-inoculatione1
Outcome
grains per plante1
Direction
increasede1
Value
11%e1
Comparator
non-inoculated referencee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e1

“Co-inoculation increased wheat grain output by 36%, grains per plant by 11%, and 1000-grain weight by 17% compared to the non-inoculated reference”

✓ located verbatim in the source · abstract, characters 429–575 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased wheat 1000-grain weight by 17% compared with the non-inoculated reference.

Treatment
Co-inoculatione1
Outcome
1000-grain weighte1
Direction
increasede1
Value
17%e1
Comparator
non-inoculated referencee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e1

“Co-inoculation increased wheat grain output by 36%, grains per plant by 11%, and 1000-grain weight by 17% compared to the non-inoculated reference”

✓ located verbatim in the source · abstract, characters 429–575 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased crop growth by 6.3% during tillering.

Organ / stage
tilleringe2
Treatment
Co-inoculatione1
Outcome
Crop growthe2
Direction
increasede2
Value
6.3%e2
Comparator
non-inoculated referencee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e1

“Co-inoculation increased wheat grain output by 36%, grains per plant by 11%, and 1000-grain weight by 17% compared to the non-inoculated reference”

✓ located verbatim in the source · abstract, characters 429–575 · show in text ↑
e2

“Crop growth increased by 6.3% during tillering and 37% at flowering”

✓ located verbatim in the source · abstract, characters 577–644 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased crop growth by 37% at flowering.

Organ / stage
at floweringe2
Treatment
Co-inoculatione1
Outcome
Crop growthe2
Direction
increasede2
Value
37%e2
Comparator
non-inoculated referencee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e1

“Co-inoculation increased wheat grain output by 36%, grains per plant by 11%, and 1000-grain weight by 17% compared to the non-inoculated reference”

✓ located verbatim in the source · abstract, characters 429–575 · show in text ↑
e2

“Crop growth increased by 6.3% during tillering and 37% at flowering”

✓ located verbatim in the source · abstract, characters 577–644 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T3 (co-inoculation) outperformed T1 (A. lipoferum alone) by 9%.

Treatment
T3 (co-inoculation)e4
Interactor
Azospirillum lipoferumAzospirillum lipoferume4symbiont
Outcome
outperformede3
Direction
outperformede3
Value
9%e3
Comparator
T1 (Azospirillum lipoferum)e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e3

“T3 outperformed T1 and T2 inoculations by 9% and 14%, respectively”

✓ located verbatim in the source · abstract, characters 646–712 · show in text ↑
e4

“T1 (Azospirillum lipoferum), T2 (Agrobacterium fabrum), and T3 (co-inoculation)”

✓ located verbatim in the source · abstract, characters 290–369 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T3 (co-inoculation) outperformed T2 (A. fabrum alone) by 14%.

Treatment
T3 (co-inoculation)e4
Interactor
Agrobacterium fabrumAgrobacterium fabrume4symbiont
Outcome
outperformede3
Direction
outperformede3
Value
14%e3
Comparator
T2 (Agrobacterium fabrum)e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e3

“T3 outperformed T1 and T2 inoculations by 9% and 14%, respectively”

✓ located verbatim in the source · abstract, characters 646–712 · show in text ↑
e4

“T1 (Azospirillum lipoferum), T2 (Agrobacterium fabrum), and T3 (co-inoculation)”

✓ located verbatim in the source · abstract, characters 290–369 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased leaf epicuticular wax and relative water content compared with the control treatment.

Organ / stage
leafe5
Treatment
co-inoculatione5
Outcome
leaf epicuticular wax and relative water contente5
Direction
increasede5
Comparator
control treatmente5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e5

“Compared to the control treatment, co-inoculation increased leaf epicuticular wax and relative water content”

✓ located verbatim in the source · abstract, characters 714–822 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Inoculating wheat seeds with A. lipoferum and A. fabrum, separately or together, may improve wheat growth, yield and quality.

Treatment
inoculating wheat seeds with A. lipoferum and A. fabrume6
Interactor
A. lipoferumAzospirillum lipoferume6symbiont
Second interactor
A. fabrumAgrobacterium fabrume6symbiont
Outcome
growth, yield, and qualitye6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Enhancing Wheat Growth”

✓ located verbatim in the source · title, characters 0–22 · show in text ↑
e6

“inoculating wheat seeds with A. lipoferum and A. fabrum separately and together may improve wheat growth, yield, and quality”

✓ located verbatim in the source · abstract, characters 836–960 · show in text ↑

inoculation · Azospirillum lipoferum · Triticum aestivum (wheat)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

inoculation · physiology: water · Triticum aestivum (wheat)Positive and negative results

35 papers: 32 positive, 3 negative or no effect

31 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. negative
  3. negative

31 papers more in this group

See all 35 papers →

inoculation · growth · Triticum aestivum (wheat)Positive and negative results

532 papers: 476 positive, 56 negative or no effect

475 papers in the same direction as this paper · 55 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

528 papers more in this group

See all 532 papers →

inoculation · yield · Triticum aestivum (wheat)Positive and negative results

614 papers: 549 positive, 65 negative or no effect

548 papers in the same direction as this paper · 60 in the opposite direction · 5 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

610 papers more in this group

See all 614 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.