Abstract
The production of seedless watermelons, primarily through triploid varieties, has surged to meet the growing consumer demand, especially due to the convenience of eating. However, triploid watermelon production is time-consuming, and seed production is tedious. Hence, in vitro propagation has become an alternative, but it faces challenges such as low regeneration response, poor rooting, and low ex vitro establishment. These issues can be addressed by applying aromatic cytokinin meta-topolin (mT) in the regeneration system. Hence, this study aims to investigate the efficacy of meta-topolin (mT) compared to 6-benzyl adenine (BA) for in vitro regeneration and acclimatization of Citrullus lanatus (Thunb.). The effects of aromatic cytokinins BA and mT (0.5, 1.0, 1.5, 2.0, and 2.5 mg/L) on multiple shoot production from cotyledonary node explants of watermelon were evaluated. The highest shoot production (25.24 shoots/explant) was observed with 1.5 mg/L mT, while BA (1.0 mg/L) produced 11.36 shoots/explant. Rooting response in MS medium with indole-3-butyric acid (IBA 0.5 to 2.5 mg/L) showed the best results in IBA 1.0 mg/L with mT-derived shoots (1.5 mg/L), producing 13.33 roots per shoot, compared to 5.62 roots per shoot from BA-derived shoots (1.0 mg/L). After four weeks of acclimatization, mT-derived plants had a 97% survival, while BA-derived plants had 84%. Additionally, mT-derived plants had significantly higher photosynthetic pigments (chlorophyll a (9.2%), b (11.3%), and carotenoids (29.1%)). RAPD and SCoT markers confirmed the genetic stability of the regenerated plants. This research will aid in developing high-quality planting material to produce commercial triploid watermelon plants.
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Claims 7
Claim · model-written summary
At 1.5 mg/L, mT produced 25.24 shoots per explant versus 11.36 with 1.0 mg/L BA.
- Taxon
- Watermelon
Citrullus lanatust0 - Organ / stage
- shoote0
- Treatment
- 1.5 mg/L mTe0
- Outcome
- shoot productione0
- Direction
- higheste0
- Value
- 25.24 shoots/explante0
- Qualifier
- 11.36 shoots/explante0
- Comparator
- BAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“The highest shoot production (25.24 shoots/explant) was observed with 1.5 mg/L mT, while BA (1.0 mg/L) produced 11.36 shoots/explant.”
Claim · model-written summary
IBA at 1.0 mg/L gave 13.33 roots per mT-derived shoot versus 5.62 for BA-derived shoots.
- Taxon
- WatermelonCitrullus lanatust0
- Organ / stage
- shootse0
- Treatment
- IBA 1.0 mg/L with mT-derived shoots (1.5 mg/L)e0
- Outcome
- Rooting responsee0
- Direction
- beste0
- Value
- 13.33 roots per shoote0
- Qualifier
- 5.62 roots per shoote0
- Comparator
- BA-derived shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“Rooting response in MS medium with indole-3-butyric acid (IBA 0.5 to 2.5 mg/L) showed the best results in IBA 1.0 mg/L with mT-derived shoots (1.5 mg/L), producing 13.33 roots per shoot, compared to 5.62 roots per shoot from BA-derived shoots (1.0 mg/L).”
Claim · model-written summary
After four weeks, mT-derived plants survived at 97% versus 84% of BA-derived plants.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- mT-derived plantse0
- Outcome
- survivale0
- Value
- 97%e0
- Qualifier
- 84%e0
- Comparator
- BA-derived plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“After four weeks of acclimatization, mT-derived plants had a 97% survival, while BA-derived plants had 84%.”
Claim · model-written summary
mT-derived plants had significantly higher chlorophyll a by 9.2%.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- mT-derived plantse0
- Outcome
- chlorophyll ae0
- Direction
- highere0
- Value
- 9.2%e0
- Comparator
- BA-derived plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“mT-derived plants had significantly higher photosynthetic pigments (chlorophyll a (9.2%), b (11.3%), and carotenoids (29.1%)).”
e1“BA-derived plants had 84%”
Claim · model-written summary
mT-derived plants had significantly higher chlorophyll b by 11.3%.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- mT-derived plantse0
- Outcome
- be0
- Direction
- highere0
- Value
- 11.3%e0
- Comparator
- BA-derived plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“mT-derived plants had significantly higher photosynthetic pigments (chlorophyll a (9.2%), b (11.3%), and carotenoids (29.1%)).”
e1“BA-derived plants had 84%”
Claim · model-written summary
mT-derived plants had significantly higher carotenoids by 29.1%.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- mT-derived plantse0
- Outcome
- carotenoidse0
- Direction
- highere0
- Value
- 29.1%e0
- Comparator
- BA-derived plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“mT-derived plants had significantly higher photosynthetic pigments (chlorophyll a (9.2%), b (11.3%), and carotenoids (29.1%)).”
e1“BA-derived plants had 84%”
Claim · model-written summary
RAPD and SCoT markers confirmed genetic stability of regenerated plants.
- Taxon
- WatermelonCitrullus lanatust0
- Outcome
- genetic stability of the regenerated plantse0
- Qualifier
- RAPD and SCoT markerse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon (Citrullus lanatus Thunb.)”
e0“RAPD and SCoT markers confirmed the genetic stability of the regenerated plants.”
The same questions in other papers
indole-3-butyric acid · growth · Citrullus lanatus (watermelon)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.