Abstract
The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About 72% of the regenerated plantlets acclimatized successfully and survived in the soil condition.
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Claims 7
Claim · model-written summary
MS medium with 5 mg l-1 BA and 0.1 mg l-1 IAA yielded maximum shoot tip growth response of 73%.
- Taxon
- watermelon
Citrullus lanatust0 - Treatment
- MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA)e0
- Outcome
- shoot tip showed growth responsee0
- Direction
- Maximume0
- Value
- 73%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA)”
t0“watermelon”
Claim · model-written summary
Cytokinin enriched medium produced multiple shoots.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- cytokinin-enriched mediume0
- Outcome
- multiple shootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots”
t0“watermelon”
Claim · model-written summary
For multiple shoot production, 2.0 mg l-1 BA was optimum.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- 2.0 mg l-1 BAe0
- Outcome
- multiple shootse1
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.0 mg l-1 BA was optimum in this respect”
e1“the cultures produced multiple shoots”
t0“watermelon”
Claim · model-written summary
Adding GA3 to multiplication medium improved shoot growth.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- Addition of gibberellic acid (GA3)e0
- Outcome
- growth of shootse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots”
t0“watermelon”
Claim · model-written summary
Second-subculture shoots cultured in 1.0 mg l-1 IBA rooted at 100%.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- medium with 1.0 mg l-1 indole-3 butyric acid (IBA)e0
- Outcome
- Rooting ratee0
- Value
- 100%e0
- Qualifier
- second subculturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA)”
t0“watermelon”
Claim · model-written summary
Number of roots increased with successive subcultures.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- increasing number of subculturese0
- Outcome
- rootse0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoots produced more roots with increasing number of subcultures”
t0“watermelon”
Claim · model-written summary
Approximately 72% of regenerated plantlets acclimatized and survived in soil.
- Taxon
- watermelonCitrullus lanatust0
- Outcome
- regenerated plantlets acclimatized successfully and survivede0
- Value
- 72%e0
- Setting
- soil conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“About 72% of the regenerated plantlets acclimatized successfully and survived in the soil condition”
t0“watermelon”
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