Abstract
Bioengineering efforts to increase oil in non-storage vegetative tissues, which constitute the majority of plant biomass, are promising sustainable sources of renewable fuels and feedstocks. While plants typically do not accumulate significant amounts of triacylglycerol (TAG) in vegetative tissues, we report here that the expression of a plastid-localized phospholipase A1 protein, DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), led to a substantial increase in leaf TAG in Arabidopsis. Using an inducible system to control DAD1 expression circumvented growth penalties associated with overexpressing DAD1 and resulted in a rapid burst of TAG within several hours. The increase of TAG was accompanied by the formation of oil bodies in the leaves, petioles, and stems, but not in the roots. Lipid analysis indicated that the increase in TAG was negatively correlated with plastidial galactolipid concentration. The fatty acid (FA) composition of TAG predominantly consisted of 18:3. Expression of DAD1 in the fad3fad7fad8 mutant, devoid of 18:3, resulted in comparable TAG accumulation with 18:2 as the major FA constituent, reflecting the flexible in vivo substrate use of DAD1. The transient expression of either Arabidopsis DAD1 or Nicotiana benthamiana DAD1 (NbDAD1) in N. benthamiana leaves stimulated the accumulation of TAG. Similarly, transgenic soybeans expressing Arabidopsis DAD1 exhibited an accumulation of TAG in the leaves, showcasing the biotechnological potential of this technology. In summary, inducible expression of a plastidial lipase resulted in enhanced oil production in vegetative tissues, extending our understanding of lipid remodeling mediated by DAD1 and offering a valuable tool for metabolic engineering.
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Claims 9
Claim · model-written summary
Expression of plastid-localized DAD1 led to a substantial increase in leaf TAG in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- expression of a plastid-localized phospholipase A1 proteine0
- Gene
- DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1)e0
- Outcome
- leaf TAGe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of a plastid-localized phospholipase A1 protein, DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), led to a substantial increase in leaf TAG in Arabidopsis”
Claim · model-written summary
Using an inducible system to control DAD1 expression circumvented the growth penalties associated with overexpressing DAD1.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- inducible system to control DAD1 expressione0
- Gene
- DAD1e0
- Outcome
- growth penaltiese0
- Direction
- circumventede0
- Comparator
- overexpressing DAD1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using an inducible system to control DAD1 expression circumvented growth penalties associated with overexpressing DAD1”
e1“substantial increase in leaf TAG in Arabidopsis”
Claim · model-written summary
Inducible DAD1 expression resulted in a rapid burst of TAG within several hours.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- inducible system to control DAD1 expressione0
- Outcome
- TAGe2
- Direction
- rapid burste2
- Qualifier
- within several hourse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using an inducible system to control DAD1 expression”
e1“substantial increase in leaf TAG in Arabidopsis”
e2“resulted in a rapid burst of TAG within several hours”
Claim · model-written summary
The TAG increase was accompanied by oil body formation in leaves, petioles and stems but not in roots.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- leaves, petioles, and stemse0
- Outcome
- formation of oil bodiese0
- Qualifier
- not in the rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase of TAG was accompanied by the formation of oil bodies in the leaves, petioles, and stems, but not in the roots”
e1“substantial increase in leaf TAG in Arabidopsis”
Claim · model-written summary
The increase in TAG was negatively correlated with plastidial galactolipid concentration.
- Taxon
- ArabidopsisArabidopsise1
- Compound
- plastidial galactolipide0
- Outcome
- increase in TAGe0
- Direction
- negatively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the increase in TAG was negatively correlated with plastidial galactolipid concentration”
e1“substantial increase in leaf TAG in Arabidopsis”
Claim · model-written summary
The fatty acid composition of TAG predominantly consisted of 18:3.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- fatty acid (FA) composition of TAGe0
- Qualifier
- predominantly consisted of 18:3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fatty acid (FA) composition of TAG predominantly consisted of 18:3”
e1“substantial increase in leaf TAG in Arabidopsis”
Claim · model-written summary
DAD1 expression in the fad3fad7fad8 mutant, devoid of 18:3, gave comparable TAG accumulation with 18:2 as the major fatty acid.
- Taxon
- ArabidopsisArabidopsise1
- Material
- fad3fad7fad8 mutante0
- Treatment
- Expression of DAD1e0
- Outcome
- TAG accumulatione0
- Direction
- comparablee0
- Qualifier
- 18:2 as the major FA constituente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of DAD1 in the fad3fad7fad8 mutant, devoid of 18:3, resulted in comparable TAG accumulation with 18:2 as the major FA constituent”
e1“substantial increase in leaf TAG in Arabidopsis”
Claim · model-written summary
Transient expression of Arabidopsis DAD1 or NbDAD1 in N. benthamiana leaves stimulated TAG accumulation.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Organ / stage
- leavese0
- Treatment
- transient expression of either Arabidopsis DAD1 or Nicotiana benthamiana DAD1 (NbDAD1)e0
- Outcome
- accumulation of TAGe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transient expression of either Arabidopsis DAD1 or Nicotiana benthamiana DAD1 (NbDAD1) in N. benthamiana leaves stimulated the accumulation of TAG”
Claim · model-written summary
Transgenic soybeans expressing Arabidopsis DAD1 accumulated TAG in leaves.
- Taxon
- soybeans
Glycine maxe0 - Organ / stage
- leavese0
- Treatment
- transgenic soybeans expressing Arabidopsis DAD1e0
- Outcome
- TAGe0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic soybeans expressing Arabidopsis DAD1 exhibited an accumulation of TAG in the leaves”
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See all 45 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.