Abstract
Oomycete pathogens deliver a number of RxLR effector proteins into host plant cells to manipulate immunity by targeting diverse host proteins. Here, we reveal that a Phytophthora infestans RxLR effector Pi18609 targets a potato B-box transcription factor (BBX), StBBX27, which was identified to positively regulate late blight resistance. Silencing the ortholog NbBBX27 in Nicotiana benthamiana or RNA interfering StBBX27 in potato increases host colonization by P. infestans, whereas transient expression of StBBX27 in N. benthamiana or stable overexpression of StBBX27 in potato enhances late blight resistance. Overexpression of StBBX27 in potato activates plant immune responses including upregulation of defense-related genes such as StWRKY8, StOSML13, StCHTB4, and StSERK2, and burst of reactive oxygen species (ROS). In addition, StBBX27 directly binds to the G-box of the WRKY8 and SERK2 promoters and activates their expression, which could be suppressed by Pi18609. Furthermore, we revealed that a ubiquitin E3 ligase COP1 promotes StBBX27 turnover by a proteasome-mediated system. Moreover, Pi18609 promotes the degradation of StBBX27 mediated by COP1. Collectively, this study reveals that StBBX27 positively regulates potato immunity and that is suppressed by effector Pi18609 to promote its turnover. This research extends our knowledge on BBXs function and the mechanisms behind P. infestans effectors suppression of host immunity.
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Claims 12
Claim · model-written summary
Pi18609 targets potato StBBX27.
- Taxon
- potato
Solanum tuberosume0 - Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Gene
- StBBX27e0
- Outcome
- targets a potato B-box transcription factor (BBX), StBBX27e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a Phytophthora infestans RxLR effector Pi18609 targets a potato B-box transcription factor (BBX), StBBX27”
Claim · model-written summary
StBBX27 positively regulates late blight resistance.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- StBBX27e0
- Outcome
- late blight resistancee0
- Direction
- positivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StBBX27, which was identified to positively regulate late blight resistance.”
e1“potato increases host colonization by P. infestans”
Claim · model-written summary
Silencing NbBBX27 in Nicotiana benthamiana increased P. infestans colonization.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Silencinge0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- NbBBX27e0
- Outcome
- host colonization by P. infestanse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing the ortholog NbBBX27 in Nicotiana benthamiana or RNA interfering StBBX27 in potato increases host colonization by P. infestans”
Claim · model-written summary
RNA interference of StBBX27 in potato increased P. infestans colonization.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- RNA interfering StBBX27e0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StBBX27e0
- Outcome
- host colonization by P. infestanse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing the ortholog NbBBX27 in Nicotiana benthamiana or RNA interfering StBBX27 in potato increases host colonization by P. infestans”
Claim · model-written summary
Transient StBBX27 expression in N. benthamiana enhanced late blight resistance.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transient expression of StBBX27e0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- StBBX27e0
- Outcome
- late blight resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of StBBX27 in N. benthamiana or stable overexpression of StBBX27 in potato enhances late blight resistance”
e1“host colonization by P. infestans”
Claim · model-written summary
Stable StBBX27 overexpression in potato enhanced late blight resistance.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- stable overexpression of StBBX27e0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- StBBX27e0
- Outcome
- late blight resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of StBBX27 in N. benthamiana or stable overexpression of StBBX27 in potato enhances late blight resistance”
e1“host colonization by P. infestans”
Claim · model-written summary
Potato StBBX27 overexpression upregulated StWRKY8, StOSML13, StCHTB4 and StSERK2.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Overexpression of StBBX27e0
- Gene
- StBBX27e0
- Outcome
- upregulation of defense-related genese0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of StBBX27 in potato activates plant immune responses including upregulation of defense-related genes such as StWRKY8, StOSML13, StCHTB4, and StSERK2”
Claim · model-written summary
Potato StBBX27 overexpression induced a reactive oxygen burst.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Overexpression of StBBX27e0
- Gene
- StBBX27e0
- Outcome
- burst of reactive oxygen species (ROS)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of StBBX27 in potato activates plant immune responses including upregulation of defense-related genes such as StWRKY8, StOSML13, StCHTB4, and StSERK2, and burst of reactive oxygen species (ROS).”
Claim · model-written summary
StBBX27 directly bound WRKY8 and SERK2 promoter G-boxes and activated expression.
- Taxon
- potatoSolanum tuberosume1
- Gene
- StBBX27e0
- Outcome
- activates their expressione0
- Qualifier
- binds to the G-box of the WRKY8 and SERK2 promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StBBX27 directly binds to the G-box of the WRKY8 and SERK2 promoters and activates their expression”
e1“a potato B-box transcription factor (BBX)”
Claim · model-written summary
Pi18609 suppressed StBBX27-mediated activation of WRKY8 and SERK2 expression.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- StBBX27e0
- Outcome
- suppressed by Pi18609e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StBBX27 directly binds to the G-box of the WRKY8 and SERK2 promoters and activates their expression, which could be suppressed by Pi18609.”
e1“potato increases host colonization by P. infestans”
Claim · model-written summary
COP1 promoted proteasome-mediated StBBX27 turnover.
- Taxon
- potatoSolanum tuberosume1
- Gene
- COP1e0
- Outcome
- StBBX27 turnovere0
- Qualifier
- proteasome-mediated systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a ubiquitin E3 ligase COP1 promotes StBBX27 turnover by a proteasome-mediated system”
e1“a potato B-box transcription factor (BBX)”
Claim · model-written summary
Pi18609 promoted COP1-mediated StBBX27 degradation.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- Phytophthora infestansPhytophthora infestanse1pathogen
- Gene
- StBBX27e0
- Outcome
- degradation of StBBX27e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pi18609 promotes the degradation of StBBX27 mediated by COP1.”
e1“a Phytophthora infestans RxLR effector Pi18609 targets a potato”
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See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.