Paper · 2025 · Journal of Kerbala for Agricultural Sciences

Effect of media type, Cytokinins and Auxins on the formation of embryogenesis of date palm Phoenix dactylifera L. in vitro

Mohammed H. Tuaimah; Osama N. Jaffar; Manal Z. Sabti

13
claims
26
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by

Abstract

The study was carried out in the tissue culture laboratory of the Date Palm Research Center, University of Basrah in 2022-2023 Ad, three types of nutritional media were used, Murashige and Skooge,( 1962)(MS) , Woody plant media ( WPM) (Lloyd and McCown, 1981), and Gamborg, (1968) (B5) supplied with two types of cytokines Zeatin and 2ip at four concentrations for each (0, 0.5 ,1 ,2) mg L-1 and two types of auxins NAA and IBA at three concentrations for each (0, 0.1, 0.5) mg L-1 to formation embryogenesis of Barhi cultivar in vitro. The results showed that the WPM and MS media gave the highest rate of the number of embryos, which amounted to 7.45 and 7 embryos, with a significant difference from the B5 media which reached (4.92 embryos ) Also, no significant differences were observed in the number of embryos for the cytokine type trait, while the results showed that the concentrations of 0 and 0.5 mg.L-1 were excelled in the rate of the number of embryos, which amounted to 7.13 embryos, with a significant difference from the rest of the concentrations. the WPM nutrient media recorded the highest percentage of embryos, number, and length of roots, which amounted to 41.7%, 1.6 roots, and 5.48 cm, respectively, with a significant difference from the MS media, which amounted to 30% percentage of embryo, and the number and length of roots amounted to 1.36 roots and 5.06 cm, respectively, while the type of auxin did not record any significant effect on the embryo percentage and root length, while IBA was excelled in the rate The number of roots reached 1.6 roots compared with NAA concentration exceeded 0.5 mg L-1 significantly in the percentage of embryo, number of roots and their length at a rate of 48.8% and 1.7 roots and 6.125 cm compared to the control treatment, while the effect of the double and triple interactions did not record any significant differences with statistical significance

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 13

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

WPM produced 7.45 embryos, more than B5 medium.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
WPMe0
Outcome
number of embryose0
Direction
higheste0
Value
7.45e0
Comparator
B5 mediae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The results showed that the WPM and MS media gave the highest rate of the number of embryos, which amounted to 7.45 and 7 embryos, with a significant difference from the B5 media which reached (4.92 embryos )”

✓ located verbatim in the source · abstract, characters 537–745 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

MS produced 7 embryos, more than B5 medium.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
MS mediae0
Outcome
number of embryose0
Direction
higheste0
Value
7 embryose0
Comparator
B5 mediae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The results showed that the WPM and MS media gave the highest rate of the number of embryos, which amounted to 7.45 and 7 embryos, with a significant difference from the B5 media which reached (4.92 embryos )”

✓ located verbatim in the source · abstract, characters 537–745 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Cytokinin type did not significantly differ for embryo number.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
cytokine typee0
Outcome
number of embryose0
Direction
no significant differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“no significant differences were observed in the number of embryos for the cytokine type trait”

✓ located verbatim in the source · abstract, characters 752–845 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Cytokinin concentrations of 0 and 0.5 mg/L gave higher embryo numbers than other concentrations.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
0 and 0.5 mg.L-1e0
Outcome
number of embryose0
Direction
excellede0
Value
7.13 embryose0
Comparator
rest of the concentrationse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the concentrations of 0 and 0.5 mg.L-1 were excelled in the rate of the number of embryos, which amounted to 7.13 embryos, with a significant difference from the rest of the concentrations.”

✓ located verbatim in the source · abstract, characters 877–1066 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

WPM gave 41.7% embryos compared with 30% for MS.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
WPM nutrient mediae0
Outcome
percentage of embryose0
Direction
higheste0
Value
41.7%e0
Qualifier
30%e0
Comparator
MS mediae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the WPM nutrient media recorded the highest percentage of embryos, number, and length of roots, which amounted to 41.7%, 1.6 roots, and 5.48 cm, respectively, with a significant difference from the MS media, which amounted to 30% percentage of embryo, and the number and length of roots amounted to 1.36 roots and 5.06 cm, respectively”

✓ located verbatim in the source · abstract, characters 1067–1402 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

WPM gave 1.6 roots compared with 1.36 under MS.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
WPM nutrient mediae0
Outcome
number, and length of rootse0
Direction
higheste0
Value
1.6 rootse0
Qualifier
1.36 rootse0
Comparator
MS mediae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the WPM nutrient media recorded the highest percentage of embryos, number, and length of roots, which amounted to 41.7%, 1.6 roots, and 5.48 cm, respectively, with a significant difference from the MS media, which amounted to 30% percentage of embryo, and the number and length of roots amounted to 1.36 roots and 5.06 cm, respectively”

✓ located verbatim in the source · abstract, characters 1067–1402 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

WPM gave 5.48 cm root length compared with 5.06 cm under MS.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
WPM nutrient mediae0
Outcome
length of rootse0
Direction
higheste0
Value
5.48 cme0
Qualifier
5.06 cme0
Comparator
MS mediae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the WPM nutrient media recorded the highest percentage of embryos, number, and length of roots, which amounted to 41.7%, 1.6 roots, and 5.48 cm, respectively, with a significant difference from the MS media, which amounted to 30% percentage of embryo, and the number and length of roots amounted to 1.36 roots and 5.06 cm, respectively”

✓ located verbatim in the source · abstract, characters 1067–1402 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Auxin type did not significantly affect embryo percentage or root length.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
type of auxine0
Outcome
embryo percentage and root lengthe0
Direction
did not record any significant effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the type of auxin did not record any significant effect on the embryo percentage and root length”

✓ located verbatim in the source · abstract, characters 1410–1506 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IBA produced 1.6 roots, outperforming NAA.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
IBAe0
Outcome
number of rootse0
Direction
excellede0
Value
1.6 rootse0
Comparator
NAAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“IBA was excelled in the rate The number of roots reached 1.6 roots compared with NAA”

✓ located verbatim in the source · abstract, characters 1514–1598 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The 0.5 mg/L NAA treatment increased embryo percentage to 48.8% compared with control.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
NAA concentration exceeded 0.5 mg L-1e0
Outcome
percentage of embryoe0
Direction
exceedede0
Value
48.8%e0
Comparator
control treatmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NAA concentration exceeded 0.5 mg L-1 significantly in the percentage of embryo, number of roots and their length at a rate of 48.8% and 1.7 roots and 6.125 cm compared to the control treatment”

✓ located verbatim in the source · abstract, characters 1595–1788 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The NAA concentration produced 1.7 roots compared with control.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
NAA concentration exceeded 0.5 mg L-1e0
Outcome
number of rootse0
Direction
exceedede0
Value
1.7 rootse0
Comparator
control treatmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NAA concentration exceeded 0.5 mg L-1 significantly in the percentage of embryo, number of roots and their length at a rate of 48.8% and 1.7 roots and 6.125 cm compared to the control treatment”

✓ located verbatim in the source · abstract, characters 1595–1788 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The NAA concentration gave root length of 6.125 cm compared with control.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
NAA concentration exceeded 0.5 mg L-1e0
Outcome
their lengthe0
Direction
exceedede0
Value
6.125 cme0
Comparator
control treatmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NAA concentration exceeded 0.5 mg L-1 significantly in the percentage of embryo, number of roots and their length at a rate of 48.8% and 1.7 roots and 6.125 cm compared to the control treatment”

✓ located verbatim in the source · abstract, characters 1595–1788 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Double and triple interactions showed no significant differences.

Taxon
date palm Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
double and triple interactionse0
Outcome
significant differencese0
Direction
did not record any significant differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the effect of the double and triple interactions did not record any significant differences with statistical significance”

✓ located verbatim in the source · abstract, characters 1796–1917 · show in text ↑
t0

“date palm Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 81–113 · show in text ↑

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19 papers: 14 positive, 5 negative or no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.