Abstract
DNA methylation is a crucial epigenetic marker linked to plant defense responses, but its significance in fungal infection of postharvest fruits remains poorly understood. This study indicated that Trichothecium roseum inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) within 24 h post-inoculation (hpi). In addition, whole-genome bisulfite sequencing showed that genomic DNA methylation levels of muskmelon decreased by 6.15% at 24 hpi. Notably, CG sites exhibited a higher methylation level and the largest number of differentially methylated regions (DMRs). Moreover, 176 DMR-associated genes (DMGs) involved in the defense response, 134 DMGs in the ROS metabolic pathway, and 41 DMGs in phenylpropanoid metabolism were identified. The differentially expressed genes harboring differential methylation were mainly influenced by hypomethylation and exhibited elevated transcript levels, involved in phenylpropanoid biosynthesis and biosynthesis of secondary metabolites.
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Claims 7
Claim · model-written summary
Fungal inoculation increased ROS production.
- Taxon
- muskmelon
.jpg?width=60)
Cucumis meloe0 - Material
- Yujinxiange0
- Treatment
- Trichothecium roseum inoculatione0
- Interactor
- Trichothecium roseum
Trichothecium roseume0pathogen - Outcome
- ROS productione0
- Direction
- increasede0
- Qualifier
- within 24 h post-inoculation (hpi)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Trichothecium roseum inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) within 24 h post-inoculation (hpi)”
Claim · model-written summary
Fungal inoculation enhanced phenylpropanoid enzyme activity.
- Taxon
- muskmelonCucumis meloe0
- Material
- Yujinxiange0
- Treatment
- Trichothecium roseum inoculatione0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Outcome
- phenylpropanoid metabolism-related enzyme activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Trichothecium roseum inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) within 24 h post-inoculation (hpi)”
Claim · model-written summary
Fungal inoculation promoted lignin accumulation.
- Taxon
- muskmelonCucumis meloe0
- Material
- Yujinxiange0
- Treatment
- Trichothecium roseum inoculatione0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Outcome
- lignin accumulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Trichothecium roseum inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) within 24 h post-inoculation (hpi)”
Claim · model-written summary
Muskmelon genomic DNA methylation fell 6.15% at 24 hours after inoculation.
- Taxon
- muskmelonCucumis meloe0
- Treatment
- Trichothecium roseum inoculatione1
- Interactor
- Trichothecium roseumTrichothecium roseume1pathogen
- Outcome
- genomic DNA methylation levelse0
- Direction
- decreasede0
- Value
- 6.15%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genomic DNA methylation levels of muskmelon decreased by 6.15% at 24 hpi”
e1“Trichothecium roseum inoculation”
Claim · model-written summary
CG sites had higher methylation and the most DMRs.
- Taxon
- MuskmelonsCucumis melot0
- Outcome
- methylation level and the largest number of differentially methylated regions (DMRs)e0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CG sites exhibited a higher methylation level and the largest number of differentially methylated regions (DMRs)”
t0“Muskmelons”
Claim · model-written summary
The study identified 176 DMR-associated defense-response genes, 134 ROS-pathway genes, and 41 phenylpropanoid genes.
- Taxon
- MuskmelonsCucumis melot0
- Outcome
- DMR-associated genes (DMGs)e0
- Value
- 176e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“176 DMR-associated genes (DMGs) involved in the defense response, 134 DMGs in the ROS metabolic pathway, and 41 DMGs in phenylpropanoid metabolism were identified”
t0“Muskmelons”
Claim · model-written summary
Hypomethylated differentially expressed genes had elevated transcript levels and involved phenylpropanoid and secondary metabolite biosynthesis.
- Taxon
- MuskmelonsCucumis melot0
- Gene
- differentially expressed genese0
- Outcome
- transcript levelse0
- Direction
- elevatede0
- Qualifier
- involved in phenylpropanoid biosynthesis and biosynthesis of secondary metabolitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The differentially expressed genes harboring differential methylation were mainly influenced by hypomethylation and exhibited elevated transcript levels, involved in phenylpropanoid biosynthesis and biosynthesis of secondary metabolites”
t0“Muskmelons”
The same questions in other papers
Trichothecium roseum · soil: biology · Cucumis melo (muskmelon)Positive and negative results
1 in the opposite direction
- negative
Trichothecium roseum · molecular: metabolites · Cucumis melo (muskmelon)Results agree
1 paper in the same direction as this paper
- positive
inoculation · soil: biology · Cucumis melo (muskmelon)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
inoculation · molecular: metabolites · Cucumis melo (muskmelon)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.