Abstract
Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H2O2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase. However, the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transporter and mitochondria dysfunction. Taken together, our results suggest that mitochondria play crucial roles in the early defense responses of muskmelons against T. roseum infection through regulation of ROS production and energy metabolism.
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Claims 7
Claim · model-written summary
Fungal infection induced mitochondrial H2O2 accumulation in harvested muskmelon fruit.
- Taxon
- Cucumis melo
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Cucumis meloe0 - Material
- cv. Yujinxiange0
- Organ / stage
- fruitse0
- Treatment
- inoculated with Trichothecium roseume0
- Interactor
- Trichothecium roseum
Trichothecium roseume0pathogen - Outcome
- H2O2 accumulation in mitochondriae1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum”
e1“the fungal infection obviously induced the H2O2 accumulation in mitochondria”
Claim · model-written summary
Succinic dehydrogenase and cytochrome c oxidase activities were inhibited in the first 6 h after inoculation.
- Taxon
- Cucumis meloCucumis meloe0
- Treatment
- inoculated with Trichothecium roseume0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Gene
- succinic dehydrogenasee1
- Outcome
- Enzyme activitiese1
- Direction
- inhibitede1
- Qualifier
- first 6 h postinoculation (hpi)e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum”
e1“Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase”
Claim · model-written summary
H+-ATPase and Ca2+-ATPase activities were inhibited in the first 6 h after inoculation.
- Taxon
- Cucumis meloCucumis meloe0
- Treatment
- inoculated with Trichothecium roseume0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Gene
- H+-ATPasee1
- Outcome
- Enzyme activitiese1
- Direction
- inhibitede1
- Qualifier
- first 6 h postinoculation (hpi)e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum”
e1“Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase”
Claim · model-written summary
Ca2+-ATPase and H+-ATPase activities rose to a higher level at 12 h after inoculation.
- Taxon
- Cucumis meloCucumis meloe0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Outcome
- activities of Ca2+-ATPase and H+-ATPasee1
- Direction
- improved to a higher levele1
- Qualifier
- 12 hpie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum”
e1“the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi”
Claim · model-written summary
Intracellular ATP content rose to a higher level at 12 h after inoculation.
- Taxon
- Cucumis meloCucumis meloe0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Outcome
- contents of intracellular adenosine triphosphate (ATP)e1
- Direction
- improved to a higher levele1
- Qualifier
- 12 hpie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum”
e1“the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi”
Claim · model-written summary
Proteomic analysis identified 42 differentially expressed proteins involved in energy metabolism, stress responses, and redox homeostasis.
- Taxon
- Cucumis meloCucumis meloe0
- Interactor
- Trichothecium roseumTrichothecium roseume0pathogen
- Outcome
- differentially expressed proteins were identifiede1
- Value
- 42e1
- Qualifier
- energy metabolism, stress responses and redox homeostasise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum”
e1“A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transporter and mitochondria dysfunction”
Claim · model-written summary
The authors suggest mitochondria have crucial roles in early defense against T. roseum through ROS and energy metabolism regulation.
- Taxon
- muskmelonsCucumis meloe0
- Interactor
- T. roseumTrichothecium roseume0pathogen
- Outcome
- early defense responses of muskmelons against T. roseum infectione0
- Qualifier
- regulation of ROS production and energy metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our results suggest that mitochondria play crucial roles in the early defense responses of muskmelons against T. roseum infection through regulation of ROS production and energy metabolism”
The same questions in other papers
Trichothecium roseum · soil: biology · Cucumis melo (muskmelon)Positive and negative results
1 in the opposite direction
- positive
inoculation · soil: biology · Cucumis melo (muskmelon)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
inoculation · physiology: oxidative · Cucumis melo (muskmelon)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.