Abstract
This research is aimed at optimizing the methodology of clonal micropropagation of Rosa L. regenerants with a view to selecting optimal conditions for implementation of their morphogenetic potential on the example of Alan Titchmarsh and Princess Alexandra of Kent varieties. Experiments were conducted using the facilities of the Laboratory of Plant Biotechnology, Main Botanical Garden of the Russian Academy of Sciences, during the period of 2024–2025. Current-year shoot segments with a length of up to 1.5 cm containing vegetative buds were used as primary explants for initiation of growth and development in vitro. We developed an optimal sterilization scheme, allowing up to 91% of viable aseptic explants to be obtained. The conducted experiments revealed specific features of regeneration of Rosa L. explants in vitro, depending on the type and concentration of growth-regulating chemicals. The hormonal composition of nutrient media for cultivation of regenerant plants of different varieties at the micropropagation stage was optimized. The efficiency of using the MS nutrient medium (Murashige and Skoog, 1962) supplemented with 6-benzylaminopurine (BAP) and thidiazuron (TDZ) at concentrations of 0.5 mg/L and 0.1 mg/L, respectively, was established. The reproduction rate was 8.4.
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Claims 3
Claim · model-written summary
The sterilization scheme yielded up to 91% viable aseptic explants.
Verbatim
e0“We developed an optimal sterilization scheme, allowing up to 91% of viable aseptic explants to be obtained.”
e1“Rosa L. explants”
Claim · model-written summary
Rosa explant regeneration depended on growth regulator type and concentration.
- Taxon
- Rosa L.e0
- Treatment
- type and concentration of growth-regulating chemicalse0
- Outcome
- regeneration of Rosa L. explantse0
- Setting
- in vitroe0
Not reportedDirection
Verbatim
e0“specific features of regeneration of Rosa L. explants in vitro, depending on the type and concentration of growth-regulating chemicals.”
Claim · model-written summary
The optimized MS medium with BAP and TDZ supported micropropagation at a reproduction rate of 8.4.
- Taxon
- Rosa L.e1
- Treatment
- MS nutrient medium (Murashige and Skoog, 1962) supplemented with 6-benzylaminopurine (BAP) and thidiazuron (TDZ) at concentrations of 0.5 mg/L and 0.1 mg/Le0
- Outcome
- reproduction ratee0
- Value
- 8.4e0
Not reportedComparatorDirection
Verbatim
e0“The efficiency of using the MS nutrient medium (Murashige and Skoog, 1962) supplemented with 6-benzylaminopurine (BAP) and thidiazuron (TDZ) at concentrations of 0.5 mg/L and 0.1 mg/L, respectively, was established. The reproduction rate was 8.4.”
e1“Rosa L. regenerants”
The same questions in other papers
N-benzyladenine · pest · RosaPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
2 papers more in this group
See all 6 papers →Thidiazuron · pest · RosaResults agree
1 paper in the same direction as this paper
- positive
in vitro · pest · RosaResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.