Abstract
An efficient protocol was developed for micropropagation of hybrid roses by manipulating growth regulators, photoperiods, gelling agent and subculture period. Multiple shoots were achieved from nodal explants of Rosa hybrida cvs. Cri Cri, Pariser Charme and First Red on the Murashige and Skoog (MS) medium supplemented with 1.5-2.0 mg/l BA (6- benzylaminopurine), 50 mg/l Ads (adenine sulfate) with 3% (w/v) sucrose. Inclusion of indole-3-acetic acid (IAA; 0.1-0.25 mg/l) into the cytokinin-rich medium promoted high frequency of shoot multiplication. The induction of multiple shoots was also affected by photoperiod and subculture period. Higher multiplication was achieved under 16 h photoperiod in all tested cultivars. The rate of multiplication was low when photoperiod both increased or decreased. The frequency of shoot multiplication was best up to the 6th to 7th subculture and thereafter it declined. Rooting was readily achieved upon transferring the microshoots onto half-strength MS medium supplemented with 0.25 mg/l IBA (indole-3-butyric acid) and 2% (w/v) sucrose. The percentage of rooting was less on MS medium containing NAA (1-naphthalene acetic acid) or IAA as compared with IBA. More than 60% of rooted plantlets were established in the greenhouse. The in vitro raised plantlets were grown normally and flowered within one month after their transfer to open field.
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Claims 9
Claim · model-written summary
Multiple shoots were obtained from nodal explants of three hybrid rose cultivars on MS medium with BA, adenine sulfate and sucrose.
- Taxon
- hybrid roset0
- Material
- Cri Cri, Pariser Charme and First Rede0
- Organ / stage
- nodal explantse0
- Treatment
- Murashige and Skoog (MS) medium supplemented with 1.5-2.0 mg/l BA (6- benzylaminopurine), 50 mg/l Ads (adenine sulfate) with 3% (w/v) sucrosee0
- Outcome
- Multiple shootse0
Not reportedComparatorDirection
Verbatim
e0“Multiple shoots were achieved from nodal explants of Rosa hybrida cvs. Cri Cri, Pariser Charme and First Red on the Murashige and Skoog (MS) medium supplemented with 1.5-2.0 mg/l BA (6- benzylaminopurine), 50 mg/l Ads (adenine sulfate) with 3% (w/v) sucrose”
t0“hybrid rose”
Claim · model-written summary
Adding IAA to the cytokinin-rich medium promoted high frequency of shoot multiplication.
- Taxon
- hybrid roset0
- Treatment
- Inclusion of indole-3-acetic acid (IAA; 0.1-0.25 mg/l)e0
- Outcome
- frequency of shoot multiplicatione0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“Inclusion of indole-3-acetic acid (IAA; 0.1-0.25 mg/l) into the cytokinin-rich medium promoted high frequency of shoot multiplication”
t0“hybrid rose”
Claim · model-written summary
Shoot multiplication was higher under a 16 h photoperiod in all tested cultivars.
Not reportedComparator
Verbatim
e0“Higher multiplication was achieved under 16 h photoperiod in all tested cultivars”
t0“hybrid rose”
Claim · model-written summary
The multiplication rate was low when the photoperiod was either increased or decreased.
- Taxon
- hybrid roset0
- Treatment
- photoperiod both increased or decreasede0
- Outcome
- rate of multiplicatione0
- Direction
- lowe0
Not reportedComparator
Verbatim
e0“The rate of multiplication was low when photoperiod both increased or decreased”
t0“hybrid rose”
Claim · model-written summary
Shoot multiplication frequency was best up to the 6th to 7th subculture and declined thereafter.
- Taxon
- hybrid roset0
- Treatment
- 6th to 7th subculturee0
- Outcome
- frequency of shoot multiplicatione0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“The frequency of shoot multiplication was best up to the 6th to 7th subculture and thereafter it declined”
t0“hybrid rose”
Claim · model-written summary
Rooting was readily achieved on half-strength MS medium with 0.25 mg/l IBA and 2% sucrose.
- Taxon
- hybrid roset0
- Organ / stage
- microshootse0
- Treatment
- half-strength MS medium supplemented with 0.25 mg/l IBA (indole-3-butyric acid) and 2% (w/v) sucrosee0
- Outcome
- Rootinge0
Not reportedComparatorDirection
Verbatim
e0“Rooting was readily achieved upon transferring the microshoots onto half-strength MS medium supplemented with 0.25 mg/l IBA (indole-3-butyric acid) and 2% (w/v) sucrose”
t0“hybrid rose”
Claim · model-written summary
Rooting percentage was lower on MS medium with NAA or IAA than with IBA.
Verbatim
e0“The percentage of rooting was less on MS medium containing NAA (1-naphthalene acetic acid) or IAA as compared with IBA”
t0“hybrid rose”
Claim · model-written summary
More than 60% of rooted plantlets were established in the greenhouse.
Verbatim
e0“More than 60% of rooted plantlets were established in the greenhouse”
t0“hybrid rose”
Claim · model-written summary
In vitro raised plantlets grew normally and flowered within one month after transfer to the open field.
Not reportedDirection
Verbatim
e0“The in vitro raised plantlets were grown normally and flowered within one month after their transfer to open field”
t0“hybrid rose”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.