Abstract
Summary Nodal sections of Quillaja saponaria cv. Quillay, including the proximal part of leaf laminae from 2-year old trees, as well as polynodal sections (3-4 axillary buds), were cultivated in Murashige and Skoog (MS) medium in the presence of various cytokinins, viz. benzyladenine (BA; 0.1, 0.5, 2.0 and 5.0 mg/litre), thidiazuron (TDZ; 0.1 and 1.0 mg/litre), 2,4-D (0.1 mg/litre), and NAA (0.1 and 0.5 mg/litre). High levels of BA and TDZ initiated profuse branching of the axillary bud and the induction of many new shoots and also callus formation in both explant types. From a single polynodal explant, >200 new multiple shoots developed in a period of 3-4 months. Proliferation of new shoots occurred mostly under the preexisting axillary bud(s) of the section and at the proximal end of the petiole with its intact leaf attached. Shoot clusters or isolated shoots elongated in subculture in the presence of MS medium without growth regulators or formed roots when supplemented with IBA within a period of 30-60 days, leading to plantlets. Root formation occurred in 1/2 strength MS medium with added 1% sucrose and 0.6, 1.2 and 5.0 mg IBA/litre, as single growth regulator. Roots did not form in lower IBA levels (0.1 mg/litre) while the addition of phloroglucinol or thiamine did not enhance rhizogenesis besides the IBA. Despite the strong shoot initiation occurring in both nodal and polynodal explants, morphogenesis was not observed in leaf explant sections, and callus derived from nodal sections, formed green globular structures only without further differentiation.
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Claims 11
Claim · model-written summary
High BA and TDZ levels induced axillary branching, new shoots and callus in both explant types.
- Taxon
- Quillaja saponaria
Quillaja saponariat0 - Treatment
- High levels of BA and TDZe0
- Outcome
- branching of the axillary bud and the induction of many new shoots and also callus formatione0
- Direction
- initiatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High levels of BA and TDZ initiated profuse branching of the axillary bud and the induction of many new shoots and also callus formation in both explant types.”
t0“Quillaja saponaria”
Claim · model-written summary
One polynodal explant produced more than 200 shoots in 3–4 months.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Organ / stage
- polynodal explante0
- Outcome
- new multiple shootse0
- Value
- >200e0
- Qualifier
- 3-4 monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From a single polynodal explant, >200 new multiple shoots developed in a period of 3-4 months.”
t0“Quillaja saponaria”
Claim · model-written summary
New shoots proliferated mainly beneath existing axillary buds and at the proximal petiole end.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Organ / stage
- axillary bud(s)e0
- Outcome
- Proliferation of new shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proliferation of new shoots occurred mostly under the preexisting axillary bud(s) of the section and at the proximal end of the petiole with its intact leaf attached.”
t0“Quillaja saponaria”
Claim · model-written summary
Shoot clusters or isolated shoots elongated on MS medium without growth regulators.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Treatment
- MS medium without growth regulatorse0
- Outcome
- Shoot clusters or isolated shoots elongatede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot clusters or isolated shoots elongated in subculture in the presence of MS medium without growth regulators”
t0“Quillaja saponaria”
Claim · model-written summary
IBA supplementation induced roots within 30–60 days and produced plantlets.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Treatment
- IBAe0
- Outcome
- formed rootse0
- Qualifier
- 30-60 dayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“formed roots when supplemented with IBA within a period of 30-60 days, leading to plantlets.”
t0“Quillaja saponaria”
Claim · model-written summary
Half-strength MS with 1% sucrose and 0.6, 1.2 or 5.0 mg IBA/litre supported root formation.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Treatment
- 1/2 strength MS medium with added 1% sucrose and 0.6, 1.2 and 5.0 mg IBA/litree0
- Outcome
- Root formatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root formation occurred in 1/2 strength MS medium with added 1% sucrose and 0.6, 1.2 and 5.0 mg IBA/litre, as single growth regulator.”
t0“Quillaja saponaria”
Claim · model-written summary
Roots did not form at 0.1 mg/litre IBA.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Treatment
- lower IBA levels (0.1 mg/litre)e0
- Outcome
- Roots did not forme0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Roots did not form in lower IBA levels (0.1 mg/litre)”
t0“Quillaja saponaria”
Claim · model-written summary
Phloroglucinol did not enhance rhizogenesis beyond IBA.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Treatment
- phloroglucinole0
- Outcome
- rhizogenesise0
- Direction
- did not enhancee0
- Comparator
- IBAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of phloroglucinol or thiamine did not enhance rhizogenesis besides the IBA.”
t0“Quillaja saponaria”
Claim · model-written summary
Thiamine did not enhance rhizogenesis beyond IBA.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Treatment
- thiaminee0
- Outcome
- rhizogenesise0
- Direction
- did not enhancee0
- Comparator
- IBAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of phloroglucinol or thiamine did not enhance rhizogenesis besides the IBA.”
t0“Quillaja saponaria”
Claim · model-written summary
Leaf explant sections showed no morphogenesis.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Organ / stage
- leaf explant sectionse0
- Outcome
- morphogenesis was not observede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“morphogenesis was not observed in leaf explant sections”
t0“Quillaja saponaria”
Claim · model-written summary
Nodal-section callus formed green globular structures without further differentiation.
- Taxon
- Quillaja saponariaQuillaja saponariat0
- Organ / stage
- callus derived from nodal sectionse0
- Outcome
- green globular structures only without further differentiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus derived from nodal sections, formed green globular structures only without further differentiation.”
t0“Quillaja saponaria”
The same questions in other papers
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- positive
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- positive
in vitro · molecular: metabolites · Quillaja saponariaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.