Abstract
To investigate the sequence characteristics of the ZmGST31 gene and the relative expression pattern under herbicide and safener application, ZmGST31 gene was cloned from maize variety ‘Zhengdan 958' by using Reverse Transcription PCR (RT-PCR). A series of bioinformatics analyses were performed subsequently to reveal its nucleotide and coding protein sequence characteristics. Real-time fluorescence quantitative PCR (qRT-PCR) was used to study its relative expression level in root, stem, and leaf with the application of Nicosulfuron (NS) and Isoxadifen-Ethyl (IE) for 24 hours and 48 hours. The results showed that the coding sequence (CDS) of ZmGST31 was 765 bp, which encoded an stable hydrophobic protein with molecular formula of C1276H2021N331O353S4, amino acid size of 254 aa, molecular weight of 27.78 kD, and isoelectric point of 6.60. The protein was mainly localized in the cytoplasm and had no signal peptide and transmembrane structure. Based on the conserved GST_C_Tau and GST_N_Tau structural domain, it was suggested to be a member of the Tau subfamily. ZmGST31 had higher homology with Dichanthelium oligosanthes GSTU17 protein. Protein-protein interaction prediction result showed that ZmGST31 could interact with ten proteins, including two glutathione synthetases and five Glutathione peroxidase proteins. The promoter region of ZmGST31 containd a large number of Cis-acting elements such as light response and hormone response. qRT-PCR results revealed that ZmGST31 was expressed in different parts of maize seedlings, with the highest expression in roots, followed by leaves, and stems. The relative expression of ZmGST31 gene in the aboveground part of maize could be induced by the nicosulfuron and isoxadifen-ethyl, the induction effect was the strongest at 24 hours. In conclusion, the relative expression level of ZmGST31 was regulated by herbicide Nicosulfuron and safener Isoxadifen-Ethyl and might play an important role in herbicide toxicity degradation or safener-mediated herbicide toxicity degradation in maize.
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Claims 7
Claim · model-written summary
ZmGST31 has a 765 bp coding sequence encoding a 254 aa protein.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the coding sequence (CDS) of ZmGST31 was 765 bp, which encoded an stable hydrophobic protein with molecular formula of C1276H2021N331O353S4, amino acid size of 254 aa, molecular weight of 27.78 kD, and isoelectric point of 6.60.”
t0“in maize”
Claim · model-written summary
ZmGST31 had higher homology with Dichanthelium oligosanthes GSTU17 protein.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmGST31 had higher homology with Dichanthelium oligosanthes GSTU17 protein.”
t0“in maize”
Claim · model-written summary
Prediction found ZmGST31 could interact with ten proteins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein-protein interaction prediction result showed that ZmGST31 could interact with ten proteins, including two glutathione synthetases and five Glutathione peroxidase proteins.”
t0“in maize”
Claim · model-written summary
The ZmGST31 promoter contains many light and hormone response cis elements.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promoter region of ZmGST31 containd a large number of Cis-acting elements such as light response and hormone response.”
t0“in maize”
Claim · model-written summary
ZmGST31 expression in seedlings was highest in roots, followed by leaves and stems.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmGST31 was expressed in different parts of maize seedlings, with the highest expression in roots, followed by leaves, and stems.”
t0“in maize”
Claim · model-written summary
Nicosulfuron induced aboveground ZmGST31 expression, strongest at 24 hours.
- Treatment
- nicosulfurone0
- Gene
- ZmGST31e0
- Outcome
- relative expression of ZmGST31 genee0
- Direction
- inducede0
- Qualifier
- strongest at 24 hourse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative expression of ZmGST31 gene in the aboveground part of maize could be induced by the nicosulfuron and isoxadifen-ethyl, the induction effect was the strongest at 24 hours.”
t0“in maize”
Claim · model-written summary
Isoxadifen-ethyl induced aboveground ZmGST31 expression, strongest at 24 hours.
- Treatment
- isoxadifen-ethyle0
- Gene
- ZmGST31e0
- Outcome
- relative expression of ZmGST31 genee0
- Direction
- inducede0
- Qualifier
- strongest at 24 hourse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative expression of ZmGST31 gene in the aboveground part of maize could be induced by the nicosulfuron and isoxadifen-ethyl, the induction effect was the strongest at 24 hours.”
t0“in maize”
The same questions in other papers
nicosulfuron · molecular: expression · Zea mays (maize)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.