Abstract
BACKGROUND: Streptomyces spp. are known for producing bioactive compounds that suppress phytopathogens. However, previous studies have largely focused on their direct interactions with pathogens and plants, often neglecting their interactions with the broader soil microbiome. In this study, we hypothesized that these interactions are critical for effective pathogen control. We investigated a diverse collection of Streptomyces strains to select those with strong protective capabilities against tomato wilt disease caused by Ralstonia solanacearum. Leveraging a synthetic community (SynCom) established in our lab, alongside multiple in planta and in vitro co-cultivation experiments, as well as transcriptomic and metabolomic analyses, we explored the synergistic inhibitory mechanisms underlying bacterial wilt resistance facilitated by both Streptomyces and the soil microbiome. RESULTS: Our findings indicate that direct antagonism by Streptomyces is not sufficient for their biocontrol efficacy. Instead, the efficacy was associated with shifts in the rhizosphere microbiome, particularly the promotion of two native keystone taxa, CSC98 (Stenotrophomonas maltophilia) and CSC13 (Paenibacillus cellulositrophicus). In vitro co-cultivation experiments revealed that CSC98 and CSC13 did not directly inhibit the pathogen. Instead, the metabolite of CSC13 significantly enhanced the inhibition efficiency of Streptomyces R02, a highly effective biocontrol strain in natural soil. Transcriptomic and metabolomic analyses revealed that CSC13's metabolites induced the production of Erythromycin E in Streptomyces R02, a key compound that directly suppressed R. solanacearum, as demonstrated by our antagonism tests. CONCLUSIONS: Collectively, our study reveals how beneficial microbes engage with the native soil microbiome to combat pathogens, suggesting the potential of leveraging microbial interactions to enhance biocontrol efficiency. These findings highlight the significance of intricate microbial interactions within the microbiome in regulating plant diseases and provide a theoretical foundation for devising efficacious biocontrol strategies in sustainable agriculture. Video Abstract.
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Claims 7
Claim · model-written summary
Direct antagonism by Streptomyces alone was insufficient for biocontrol efficacy against tomato wilt.
- Taxon
- tomato
Solanum lycopersicumt0 - Interactor
- Streptomyces
Streptomycese0biocontrol_agent - Outcome
- biocontrol efficacye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“direct antagonism by Streptomyces is not sufficient for their biocontrol efficacy”
t0“tomato wilt disease caused by Ralstonia solanacearum”
Claim · model-written summary
Biocontrol efficacy was associated with rhizosphere shifts promoting CSC98 and CSC13.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- Stenotrophomonas maltophilia
Stenotrophomonas maltophiliae0biocontrol_agent - Second interactor
- Paenibacillus cellulositrophicusPaenibacillus cellulositrophicuse0biocontrol_agent
- Outcome
- efficacye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the efficacy was associated with shifts in the rhizosphere microbiome, particularly the promotion of two native keystone taxa, CSC98 (Stenotrophomonas maltophilia) and CSC13 (Paenibacillus cellulositrophicus)”
t0“tomato wilt disease caused by Ralstonia solanacearum”
Claim · model-written summary
CSC98 did not directly inhibit the tomato wilt pathogen in vitro.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- CSC98e0biocontrol_agent
- Outcome
- CSC98 and CSC13 did not directly inhibit the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CSC98 and CSC13 did not directly inhibit the pathogen”
t0“tomato wilt disease caused by Ralstonia solanacearum”
Claim · model-written summary
CSC13 did not directly inhibit the tomato wilt pathogen in vitro.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- CSC13e0biocontrol_agent
- Outcome
- CSC13 did not directly inhibit the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CSC98 and CSC13 did not directly inhibit the pathogen”
t0“tomato wilt disease caused by Ralstonia solanacearum”
Claim · model-written summary
CSC13 metabolite significantly enhanced the pathogen inhibition efficiency of Streptomyces R02.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- the metabolite of CSC13e0
- Interactor
- Streptomyces R02e0biocontrol_agent
- Second interactor
- CSC13e0biocontrol_agent
- Outcome
- inhibition efficiency of Streptomyces R02e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the metabolite of CSC13 significantly enhanced the inhibition efficiency of Streptomyces R02”
t0“tomato wilt disease caused by Ralstonia solanacearum”
Claim · model-written summary
CSC13 metabolites induced Erythromycin E production in Streptomyces R02.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- Streptomyces R02e0biocontrol_agent
- Outcome
- production of Erythromycin E in Streptomyces R02e0
- Qualifier
- CSC13's metabolitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CSC13's metabolites induced the production of Erythromycin E in Streptomyces R02”
t0“tomato wilt disease caused by Ralstonia solanacearum”
Claim · model-written summary
Erythromycin E produced by Streptomyces R02 directly suppressed R. solanacearum.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Second interactor
- Streptomyces R02e0biocontrol_agent
- Compound
- Erythromycin Ee0
- Outcome
- suppressed R. solanacearume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Erythromycin E in Streptomyces R02, a key compound that directly suppressed R. solanacearum”
t0“tomato wilt disease caused by Ralstonia solanacearum”
The same questions in other papers
metabolite · disease · Solanum lycopersicum (tomato)Results agree
4 papers in the same direction as this paper
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- positive
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