Paper · 2004 · Journal of the American Society for Horticultural Science

Factors Affecting Seed Production in Transgenic Ethylene-insensitive Petunias

Donna J. Clevenger; James E. Barrett; Harry J. Klee; et al.

8
claims
16
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

Pollen viability, in-vivo pollen tube growth, fruit ripening, seed germination, seed weight, whole plant vigor, and natural flower senescence were investigated in homozygous and heterozygous transgenic ethylene-insensitive CaMV35S:: etr1-1 petunias ( Petunia × hybrida `Mitchell Diploid'). Homozygous or heterozygous plants were used to determine any maternal and/or paternal effects of the CaMV35S:: etr1-1 transgene. All experiments except for those used to determine natural flower senescence characteristics were conducted in both high and low temperature greenhouses to determine the effect of temperature stress on transgenic plants when compared to wild-type. Results indicated that ethylene-insensitive plants had a decrease in pollen viability, root dry mass, seed weight, and seed germination. Fruit ripening, seed germination, and seed weight were maternally regulated. In contrast, the CaMV35S:: etr1-1 transgene is completely dominant in its effect on natural flower senescence.

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Claims 8

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Ethylene-insensitive petunias had reduced pollen viability.

Taxon
petuniasPetuniat0
Material
ethylene-insensitive plantse0
Outcome
pollen viabilitye0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“ethylene-insensitive plants had a decrease in pollen viability, root dry mass, seed weight, and seed germination”

✓ located verbatim in the source · abstract, characters 690–802 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Ethylene-insensitive petunias had reduced root dry mass.

Taxon
petuniasPetuniat0
Material
ethylene-insensitive plantse0
Outcome
root dry masse0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“ethylene-insensitive plants had a decrease in pollen viability, root dry mass, seed weight, and seed germination”

✓ located verbatim in the source · abstract, characters 690–802 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Ethylene-insensitive petunias had reduced seed weight.

Taxon
petuniasPetuniat0
Material
ethylene-insensitive plantse0
Outcome
seed weighte0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“ethylene-insensitive plants had a decrease in pollen viability, root dry mass, seed weight, and seed germination”

✓ located verbatim in the source · abstract, characters 690–802 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Ethylene-insensitive petunias had reduced seed germination.

Taxon
petuniasPetuniat0
Material
ethylene-insensitive plantse0
Outcome
seed germinatione0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“ethylene-insensitive plants had a decrease in pollen viability, root dry mass, seed weight, and seed germination”

✓ located verbatim in the source · abstract, characters 690–802 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fruit ripening was maternally regulated.

Taxon
petuniasPetuniat0
Outcome
Fruit ripeninge0
Qualifier
maternally regulatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Fruit ripening, seed germination, and seed weight were maternally regulated”

✓ located verbatim in the source · abstract, characters 804–879 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seed germination was maternally regulated.

Taxon
petuniasPetuniat0
Outcome
seed germinatione0
Qualifier
maternally regulatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Fruit ripening, seed germination, and seed weight were maternally regulated”

✓ located verbatim in the source · abstract, characters 804–879 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seed weight was maternally regulated.

Taxon
petuniasPetuniat0
Outcome
seed weighte0
Qualifier
maternally regulatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Fruit ripening, seed germination, and seed weight were maternally regulated”

✓ located verbatim in the source · abstract, characters 804–879 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The CaMV35S:: etr1-1 transgene was completely dominant for natural flower senescence.

Taxon
petuniasPetuniat0
Gene
CaMV35S:: etr1-1e0
Outcome
natural flower senescencee0
Qualifier
completely dominante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“the CaMV35S:: etr1-1 transgene is completely dominant in its effect on natural flower senescence”

✓ located verbatim in the source · abstract, characters 894–990 · show in text ↑
t0

“Petunias”

✓ located verbatim in the source · title, characters 69–77 · show in text ↑