Abstract
Heat stress significantly reduces canola seed production during the post-pollination stages. This study explored changes in the proteome of flowers on the main stem of three Brassica napus cultivars exposed to transient heat stress after pollination. Flowers at the 2nd to 5th reproductive nodes on the main stem were collected on days 0, 1, 3 and 6 of heat stress and control treatments. The three cultivars, Alku, AV-Ruby, and YM11, exhibited varying degrees of heat sensitivity and resilience based on seed production in pods at these reproductive nodes. The seed yield per pod under heat stress was 75.3 % of the control in Alku and 64.2 % in YM11. However, AV-Ruby retained 93.5 % of its seed yield under heat stress, from which we conclude AV-Ruby was more resilient to heat stress during the post-pollination stage than Alku or YM11. There were 474 differentially abundant proteins (DAPs) identified across all cultivars and time points. Among the DAPs, two HSP20-like chaperones (A0A078I8F7, A0A078JBL3) and one HSP-related protein (A0A078JJT8) were consistently highly abundant under heat and were strong candidates as heat responsive proteins. Pathways related to maintaining membrane integrity were specifically enriched in AV-Ruby, and deserve further study for their potential involvement in heat tolerance. SIGNIFICANCE OF THE STUDY: Heat stress is a major factor threatening seed yield in cool season crops including oilseed rape, particularly during the post-pollination stages when pollination, fertilization, and embryo development are highly vulnerable to elevated temperatures. A comparative proteomic analysis was carried out to identify heat-responsive proteins during the post-pollination period. Among the DAPs, three were consistently associated with heat stress response. A range of biological processes, including protein folding and stress signalling, were involved in a general response to heat stress in all cultivars. Furthermore, pathways related to maintaining membrane integrity were specifically enhanced in a heat-resilient cultivar. These findings provide new insights into the heat response at the protein level and lay the groundwork for identifying potential molecular targets for breeding heat-tolerant oilseed rape cultivars.
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Claims 8
Claim · model-written summary
Alku seed yield per pod under heat was 75.3 % of control.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Material
- Alkue0
- Treatment
- heat stresse0
- Outcome
- seed yield per pode0
- Value
- 75.3 %e0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“Brassica napus”
e0“The seed yield per pod under heat stress was 75.3 % of the control in Alku”
Claim · model-written summary
YM11 seed yield per pod under heat was 64.2 % of control.
- Taxon
- Brassica napusBrassica napust0
- Material
- YM11e0
- Treatment
- heat stresse1
- Outcome
- seed yield per pode1
- Value
- 64.2 %e0
- Comparator
- controle1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“64.2 % in YM11”
e1“The seed yield per pod under heat stress was 75.3 % of the control in Alku”
Claim · model-written summary
AV-Ruby retained 93.5 % of seed yield under heat and was more resilient than Alku or YM11.
- Taxon
- Brassica napusBrassica napust0
- Material
- AV-Rubye0
- Treatment
- heat stresse0
- Outcome
- seed yielde0
- Direction
- retainede0
- Value
- 93.5 %e0
- Comparator
- Alku or YM11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“AV-Ruby retained 93.5 % of its seed yield under heat stress, from which we conclude AV-Ruby was more resilient to heat stress during the post-pollination stage than Alku or YM11”
Claim · model-written summary
474 proteins were differentially abundant across cultivars and time points.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- differentially abundant proteinse0
- Value
- 474e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“There were 474 differentially abundant proteins (DAPs) identified across all cultivars and time points”
Claim · model-written summary
Two HSP20-like chaperones were consistently highly abundant under heat.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- heate0
- Gene
- A0A078I8F7, A0A078JBL3e0
- Outcome
- highly abundante0
- Direction
- highlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“two HSP20-like chaperones (A0A078I8F7, A0A078JBL3) and one HSP-related protein (A0A078JJT8) were consistently highly abundant under heat”
Claim · model-written summary
An HSP-related protein was consistently highly abundant under heat.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- heate0
- Gene
- A0A078JJT8e0
- Outcome
- highly abundante0
- Direction
- highlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“one HSP-related protein (A0A078JJT8) were consistently highly abundant under heat”
Claim · model-written summary
Membrane-integrity pathways were specifically enriched in AV-Ruby.
- Taxon
- Brassica napusBrassica napust0
- Material
- AV-Rubye0
- Outcome
- Pathways related to maintaining membrane integritye0
- Direction
- enrichede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“Pathways related to maintaining membrane integrity were specifically enriched in AV-Ruby”
Claim · model-written summary
Protein folding and stress signalling were involved in general heat response across cultivars.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- protein folding and stress signallinge0
- Qualifier
- heat stress in all cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“protein folding and stress signalling, were involved in a general response to heat stress in all cultivars”
The same questions in other papers
heat · yield · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 19 in the opposite direction
- negative
- negative
- negative
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.