Paper · 2025 · The Journal of Horticultural Science and Biotechnology

Optimal conditions for anther dehiscence and long-term storage of the pollen of Capsicum annuum L. ‘Amato-bijin’

Mizuki Niikura; Masako Akutsu

6
claims
12
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

Pollen collection and storage conditions are important aspects to consider for horticulturally important crops. This study aimed to determine the optimal temperature under both dry and wet conditions for in vitro anther dehiscence and the optimal temperature under vacuum, nitrogen or carbon dioxide for long-term pollen storage of ‘Amato-bijin’. When the flowers were placed under dry condition for 6 h, anther dehiscence occurred, and pollen content increased, indicating that some hours after flowering are necessary to obtain a sufficient amount of pollen. Flowers were collected and kept in dry and wet conditions. The results showed that, under wet conditions, the pollen germination rate immediately after collection was maintained for 24 h, allowing anthers to dehisce and to obtain sufficient pollen. However, when anther dehiscence occurred under dry conditions, the pollen germination rate was less than half after 1 h, indicating that moisture was necessary to maintain the pollen activity. Furthermore, the pollen germination rate could be maintained at 10 to 25°C. Pollen collected and stored at -25°C for one year maintained a pollen germination rate of more than 7%, particularly under vacuum and nitrogen conditions. These results indicate that ‘Amato-bijin’ pollen can be easily stored at -25°C for one year. .

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dry flower handling for six hours caused anther dehiscence.

Taxon
Capsicum annuum L. ‘Amato-bijin’Capsicum annuumt0
Treatment
dry condition for 6 he0
Outcome
anther dehiscence occurrede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“When the flowers were placed under dry condition for 6 h, anther dehiscence occurred, and pollen content increased”

✓ located verbatim in the source · abstract, characters 347–461 · show in text ↑
t0

“Capsicum annuum L. ‘Amato-bijin’”

✓ located verbatim in the source · title, characters 80–112 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dry flower handling for six hours increased pollen content.

Taxon
Capsicum annuum L. ‘Amato-bijin’Capsicum annuumt0
Treatment
dry condition for 6 he0
Outcome
pollen contente0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“When the flowers were placed under dry condition for 6 h, anther dehiscence occurred, and pollen content increased”

✓ located verbatim in the source · abstract, characters 347–461 · show in text ↑
t0

“Capsicum annuum L. ‘Amato-bijin’”

✓ located verbatim in the source · title, characters 80–112 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Wet conditions maintained pollen germination for 24 hours after collection.

Taxon
Capsicum annuum L. ‘Amato-bijin’Capsicum annuumt0
Treatment
wet conditionse0
Outcome
pollen germination ratee0
Direction
maintainede0
Value
24 he0
Comparator
immediately after collectione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“under wet conditions, the pollen germination rate immediately after collection was maintained for 24 h”

✓ located verbatim in the source · abstract, characters 645–747 · show in text ↑
t0

“Capsicum annuum L. ‘Amato-bijin’”

✓ located verbatim in the source · title, characters 80–112 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Under dry dehiscence conditions, pollen germination fell below half after one hour.

Taxon
Capsicum annuum L. ‘Amato-bijin’Capsicum annuumt0
Treatment
dry conditionse0
Outcome
pollen germination ratee0
Direction
less than halfe0
Value
1 he0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“when anther dehiscence occurred under dry conditions, the pollen germination rate was less than half after 1 h”

✓ located verbatim in the source · abstract, characters 819–929 · show in text ↑
t0

“Capsicum annuum L. ‘Amato-bijin’”

✓ located verbatim in the source · title, characters 80–112 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Pollen germination remained maintained at 10 to 25°C.

Taxon
Capsicum annuum L. ‘Amato-bijin’Capsicum annuumt0
Outcome
pollen germination ratee0
Direction
maintainede0
Value
10 to 25°Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the pollen germination rate could be maintained at 10 to 25°C”

✓ located verbatim in the source · abstract, characters 1016–1077 · show in text ↑
t0

“Capsicum annuum L. ‘Amato-bijin’”

✓ located verbatim in the source · title, characters 80–112 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

After a year at -25°C, pollen retained germination above 7%, especially under vacuum and nitrogen.

Taxon
Capsicum annuum L. ‘Amato-bijin’Capsicum annuumt0
Treatment
stored at -25°C for one yeare0
Outcome
pollen germination ratee0
Direction
maintainede0
Value
more than 7%e0
Qualifier
under vacuum and nitrogen conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollen collected and stored at -25°C for one year maintained a pollen germination rate of more than 7%, particularly under vacuum and nitrogen conditions”

✓ located verbatim in the source · abstract, characters 1079–1232 · show in text ↑
t0

“Capsicum annuum L. ‘Amato-bijin’”

✓ located verbatim in the source · title, characters 80–112 · show in text ↑

drought · yield · Capsicum annuum (sweet pepper)Results differ

11 papers: 3 positive, 8 negative or no effect

7 papers in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.