Paper · 2025 · Journal of Plant Nutrition

Evaluation of biostimulant and salt-mediated stress alleviating activities of tropical red and brown seaweeds based biostimulant through green gram seedling cut assay technique

Gopi Krishna Ramamoorthy; Shanmugam Munisamy

4
claims
8
verbatim quotes
2
citations
2
research areas
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Open access · cc-by

Abstract

Crop yield and quality improvements, building of resistance toward biotic and abiotic stresses and enhancing nutrient use efficiency are some of the demonstrated benefits of seaweed extracts when they applied on the crops. In the present study, a concentrated seaweed extract (CSW) was produced by a blend of red (Gracilaria salicornia) and brown seaweed (Sargassum swartzii) extracts and evaluated its biostimulant and salt mediated stress alleviating activities by using a green gram (Vigna radiata) seedling method. The CSW (soluble solids: 29.93 ± 0.84%) was tested to contain macro- and micronutrients, plant growth regulators (auxins, cytokinins, and gibberellic acid), amino acids, and alginic acids. The biostimulant activity was evaluated by growing the stem cuttings of green gram seedlings in different concentration of CSW for 10 days and roots developed were measured by a WinRhizo Pro root imaging system. The salt-mediated stress-alleviating activity of CSW was studied by growing the green gram stems in different concentration of sodium chloride, that is, 25, 50, and 75 mM along with 0.1% of CSW and control experiment was run in parallel to compare the results. It was observed that CSW at 0.1% mitigated the salt stress up to a 50 mM concentration level and promoted the normal root growth and increased the plant biomass by 18.86% over the control (p = .01). The entire assay work, except pot trial, was completed in 21 days, hence it could a useful test method to assess the biostimulant and or salt mediated stress alleviating activities of biostimulants.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

The concentrated seaweed extract contained macro- and micronutrients, plant growth regulators, amino acids, and alginic acids.

Outcome
macro- and micronutrients, plant growth regulators (auxins, cytokinins, and gibberellic acid), amino acids, and alginic acidse0
Value
29.93 ± 0.84%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The CSW (soluble solids: 29.93 ± 0.84%) was tested to contain macro- and micronutrients, plant growth regulators (auxins, cytokinins, and gibberellic acid), amino acids, and alginic acids.”

✓ located verbatim in the source · abstract, characters 519–707 · show in text ↑
e1

“green gram (Vigna radiata)”

✓ located verbatim in the source · abstract, characters 475–501 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 0.1%, CSW mitigated salt stress up to 50 mM and promoted normal root growth.

Treatment
CSW at 0.1%e0
Outcome
root growthe0
Direction
promotede0
Qualifier
up to a 50 mM concentration levele0
Comparator
control experimente2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“CSW at 0.1% mitigated the salt stress up to a 50 mM concentration level and promoted the normal root growth”

✓ located verbatim in the source · abstract, characters 1202–1309 · show in text ↑
e1

“green gram (Vigna radiata)”

✓ located verbatim in the source · abstract, characters 475–501 · show in text ↑
e2

“control experiment was run in parallel”

✓ located verbatim in the source · abstract, characters 1118–1156 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 0.1%, CSW increased green gram biomass by 18.86% over the control under salt stress.

Treatment
CSW at 0.1%e0
Outcome
plant biomasse0
Direction
increasede0
Value
18.86%e0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“CSW at 0.1% mitigated the salt stress up to a 50 mM concentration level and promoted the normal root growth and increased the plant biomass by 18.86% over the control (p = .01)”

✓ located verbatim in the source · abstract, characters 1202–1378 · show in text ↑
e1

“green gram (Vigna radiata)”

✓ located verbatim in the source · abstract, characters 475–501 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The seedling cut assay was completed in 21 days and was proposed for testing biostimulant and salt stress alleviation activity.

Outcome
assay worke0
Value
21 dayse0
Qualifier
except pot triale0

Verbatim

e0

“The entire assay work, except pot trial, was completed in 21 days, hence it could a useful test method to assess the biostimulant and or salt mediated stress alleviating activities of biostimulants.”

✓ located verbatim in the source · abstract, characters 1380–1578 · show in text ↑

other · growth · Vigna radiata (mung bean)Results agree

21 papers: 21 positive, 0 negative or no effect

20 papers in the same direction as this paper

  1. positive
  2. positive
    Seaweed extract seed soaking plus foliar sprays gave the highest plant height in greengram (56.06 cm).

    Evaluation of seaweed extract on growth determinants, yield and biochemical parameters of greengram (Vigna radiata) · 2019 · compared with seed soaking in 0.1 % seaweed extract solution for 30 minutes + foliar application of seaweed extract (0.25%) on 25 DAS (single spray)

    Same directionCited by this paper

  3. positive

17 papers more in this group

See all 21 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.