Abstract
Pinus elliottii, a key economic conifer in southern China, requires efficient propagation methods to meet demand for elite germplasm in resin and timber production. While somatic embryogenesis-based plant regeneration has been successfully achieved in Pinus elliottii, large-scale production remains challenging. Our results demonstrate that the genotype of Pinus elliottii significantly influences the induction rate of embryogenic callus. During somatic embryo maturation, the liquid–solid induction method increased the number of mature embryos by 25.85 times. Maturation efficiency was further enhanced by a 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COS. Additionally, the incorporation of activated carbon significantly promoted both the maturation and germination of somatic embryos. In the somatic embryo maturation stage, 1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus, resulting in a total of 1452 embryos. During germination, the application of 4 g/L activated carbon achieved a germination rate of 63%, and the survival rate of somatic embryo-derived seedlings reached 85%. This study not only identifies the optimal conditions for somatic embryogenesis in Pinus elliottii but also establishes an efficient protocol for somatic embryo maturation induction, providing a crucial scientific foundation for the rapid propagation and seedling production of Pinus elliottii.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
The genotype of Pinus elliottii significantly influences the induction rate of embryogenic callus.
- Taxon
- Pinus elliottii
.jpg?width=60)
Pinus elliottiie0 - Material
- genotypee1
- Outcome
- induction rate of embryogenic calluse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e1“the genotype of Pinus elliottii significantly influences the induction rate of embryogenic callus”
Claim · model-written summary
The liquid-solid induction method increased the number of mature embryos by 25.85 times.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Organ / stage
- somatic embryo maturatione2
- Treatment
- liquid–solid induction methode2
- Outcome
- number of mature embryose2
- Direction
- increasede2
- Value
- 25.85 timese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e2“During somatic embryo maturation, the liquid–solid induction method increased the number of mature embryos by 25.85 times”
Claim · model-written summary
A 3-week pretreatment followed by ABA, PSK and COS enhanced somatic embryo maturation efficiency.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Treatment
- 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COSe3
- Outcome
- Maturation efficiencye3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e3“Maturation efficiency was further enhanced by a 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COS”
Claim · model-written summary
Activated carbon significantly promoted somatic embryo maturation and germination.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Treatment
- activated carbone4
- Outcome
- maturation and germinatione4
- Direction
- promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e4“the incorporation of activated carbon significantly promoted both the maturation and germination of somatic embryos”
Claim · model-written summary
1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Treatment
- 1 g/L activated carbone5
- Outcome
- mature embryos per gram of embryogenic calluse5
- Direction
- inducede5
- Value
- 288.67e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e5“1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus”
Claim · model-written summary
1 g/L activated carbon gave a total of 1452 embryos.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Treatment
- 1 g/L activated carbone5
- Outcome
- embryose6
- Value
- 1452e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e5“1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus”
e6“resulting in a total of 1452 embryos”
Claim · model-written summary
4 g/L activated carbon achieved a germination rate of 63%.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Treatment
- 4 g/L activated carbone7
- Outcome
- germination ratee7
- Direction
- achievede7
- Value
- 63%e7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e7“the application of 4 g/L activated carbon achieved a germination rate of 63%”
Claim · model-written summary
The survival rate of somatic embryo-derived seedlings reached 85%.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Outcome
- survival rate of somatic embryo-derived seedlingse1
- Value
- 85%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pinus elliottii, a key economic conifer in southern China”
e1“the survival rate of somatic embryo-derived seedlings reached 85%”