Abstract
Plants resist pathogen invasion by triggering reactive oxygen species (ROS) burst. However, the molecular mechanism by which pathogens suppress ROS accumulation for successful infection remains to be elucidated. To address this concern, an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust, was characterized. The function of Pst9653 during Pst infection was identified by RNA interference and overexpression in wheat. The interaction targets of Pst9653 were screened by yeast two-hybrid assays and further regulatory mode was also determined. The results showed that Pst9653, encoding a virulence effector, was significantly upregulated during Pst infection. Silencing of Pst9653 led to increased wheat resistance to stripe rust and restricted fungal development, whereas overexpressing Pst9653 greatly promoted Pst susceptibility in wheat. In addition, Pst9653 was shown to interact with wheat catalases TaCAT1 and TaCAT3. TaCAT3 was induced by Pst and negatively regulated wheat resistance to stripe rust. Co-expression of TaCAT3 and Pst9653 facilitates cytoplasmic enrichment of TaCAT3 in plants. Our findings reveal that Pst9653 is secreted and disrupts TaCAT3 import into peroxisomes to promote susceptibility-involved ROS scavenging in wheat. This study uncovers a novel regulation strategy to cope with excessive ROS accumulation under stress conditions.
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Claims 9
Claim · model-written summary
Pst9653 was significantly upregulated during Pst infection of wheat.
- Taxon
- wheat
Triticum aestivume2 - Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie2pathogen - Gene
- Pst9653e0
- Outcome
- upregulated during Pst infectione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that Pst9653, encoding a virulence effector, was significantly upregulated during Pst infection”
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
Claim · model-written summary
Silencing of Pst9653 increased wheat resistance to stripe rust.
- Taxon
- wheatTriticum aestivume3
- Treatment
- Silencing of Pst9653e3
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie2pathogen
- Outcome
- wheat resistance to stripe ruste3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
e3“Silencing of Pst9653 led to increased wheat resistance to stripe rust and restricted fungal development”
Claim · model-written summary
Silencing of Pst9653 restricted fungal development in wheat.
- Taxon
- wheatTriticum aestivume3
- Treatment
- Silencing of Pst9653e3
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie2pathogen
- Outcome
- fungal developmente3
- Direction
- restrictede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
e3“Silencing of Pst9653 led to increased wheat resistance to stripe rust and restricted fungal development”
Claim · model-written summary
Overexpressing Pst9653 greatly promoted Pst susceptibility in wheat.
- Taxon
- wheatTriticum aestivume4
- Treatment
- overexpressing Pst9653e4
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie2pathogen
- Outcome
- Pst susceptibilitye4
- Direction
- greatly promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
e4“overexpressing Pst9653 greatly promoted Pst susceptibility in wheat”
Claim · model-written summary
TaCAT3 was induced by Pst in wheat.
- Taxon
- wheatTriticum aestivume5
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie2pathogen
- Gene
- TaCAT3e5
- Outcome
- TaCAT3 was induced by Pste5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
e5“TaCAT3 was induced by Pst and negatively regulated wheat resistance to stripe rust”
Claim · model-written summary
TaCAT3 negatively regulated wheat resistance to stripe rust.
- Taxon
- wheatTriticum aestivume5
- Gene
- TaCAT3e5
- Outcome
- wheat resistance to stripe ruste5
- Direction
- negatively regulatede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“TaCAT3 was induced by Pst and negatively regulated wheat resistance to stripe rust”
Claim · model-written summary
Pst9653 interacts with wheat catalases TaCAT1 and TaCAT3 in yeast two-hybrid assays.
- Taxon
- wheatTriticum aestivume6
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie2pathogen
- Gene
- TaCAT1 and TaCAT3e6
- Outcome
- interact with wheat catalasese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
e6“Pst9653 was shown to interact with wheat catalases TaCAT1 and TaCAT3”
Claim · model-written summary
Co-expression of TaCAT3 and Pst9653 facilitates cytoplasmic enrichment of TaCAT3 in wheat.
- Taxon
- wheatTriticum aestivume2
- Treatment
- Co-expression of TaCAT3 and Pst9653e7
- Gene
- TaCAT3e7
- Outcome
- cytoplasmic enrichment of TaCAT3e7
- Direction
- facilitatese7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an effector protein Pst9653 from Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust”
e7“Co-expression of TaCAT3 and Pst9653 facilitates cytoplasmic enrichment of TaCAT3 in plants”
Claim · model-written summary
Pst9653 is secreted and disrupts TaCAT3 import into peroxisomes to promote susceptibility-involved ROS scavenging in wheat.
- Taxon
- wheatTriticum aestivume7
- Gene
- TaCAT3e7
- Outcome
- TaCAT3 import into peroxisomese7
- Direction
- disruptse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Pst9653 is secreted and disrupts TaCAT3 import into peroxisomes to promote susceptibility-involved ROS scavenging in wheat”
The same questions in other papers
loss of function · Puccinia striiformis f. sp. tritici · Triticum aestivum (wheat)Positive and negative results
7 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- negative
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.