Paper · 2025 · AGRITEPA Jurnal Ilmu dan Teknologi Pertanian

The Total Flavonoid Content And Bioassay Test Characteristics Of Sorghum Extracts At Different Levels Of Drought Stress

Edi Susilo; Hesti Pujiwati

4
claims
8
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by-sa

Abstract

Purpose: to determine the total flavonoid content and seed germination characteristics in bioassay tests due to sorghum extracts' application at various drought stress levels. Extract materials were prepared from June to August 2024 at Bengkulu University Greenhouse, Kandang Limun Village, Bengkulu City, and bioassay tests were conducted in August 2024 at Pematang Gubernur, Muara Bangkahulu, Bengkulu City. Flavonoid analysis was performed at the Integrated Research and Testing Laboratory (LPPT) of Gadjah Mada University in August 2024. Methodology: This study used a completely randomized design (CRD) with one factor. Two sorghum varieties, Super 2 and Mandau, were planted with water stress treatment through watering patterns every day, every 3 days, and every 5 days. The experimental unit was a petri dish, and the experiment was repeated four times. The Petri dish bioassay method was applied in this experiment.Each petri dish was given 10 mL of water extract, and 25 mung bean seeds were planted and incubated for three days. Results: The results showed that the Mandau variety had the highest total flavonoid content with watering every 3 days (3991.34 µg/g), and the Super 2 variety had the lowest total flavonoid content with watering every day (3165.26 µg/g). The highest germination inhibition was achieved by the sorghum variety Mandau with watering every 5 days. The treatment produced the highest inhibition on the test plants, so it has the potential to be the best bioherbicide. Findings: The Mandau variety has the highest total flavonoid content with watering every 3 days, and the Super 2 variety has the lowest total flavonoid content with watering every day. Novelty: This study provides insight into the total flavonoid content and seed germination characteristics due to the application of sorghum extracts at various levels of drought stress. Originality: This study offers a detailed analysis of total flavonoid levels and seed germination characteristics in bioassay tests. Conclusion: The Mandau variety had the highest total flavonoid content with watering every 3 days. The Mandau sorghum variety achieved the highest germination inhibition with watering every 5 days. Type of Paper: empirical research article.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Mandau under watering every 3 days had the highest flavonoid content, 3991.34 µg/g.

Taxon
SorghumSorghumt0
Material
Mandaue0
Treatment
watering every 3 dayse0
Outcome
total flavonoid contente0
Direction
higheste0
Value
3991.34 µg/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the Mandau variety had the highest total flavonoid content with watering every 3 days (3991.34 µg/g)”

✓ located verbatim in the source · abstract, characters 1073–1173 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 65–72 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Super 2 under daily watering had the lowest flavonoid content, 3165.26 µg/g.

Taxon
SorghumSorghumt0
Material
Super 2e0
Treatment
watering every daye0
Outcome
total flavonoid contente0
Direction
loweste0
Value
3165.26 µg/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the Super 2 variety had the lowest total flavonoid content with watering every day (3165.26 µg/g)”

✓ located verbatim in the source · abstract, characters 1179–1276 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 65–72 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Mandau extract from five day watering had the highest mung bean germination inhibition.

Taxon
mung beanVigna radiatae1
Treatment
sorghum variety Mandau with watering every 5 dayse0
Interactor
sorghumSorghume0other
Outcome
germination inhibitione0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗
Sorghum
Sorghum · Interactor · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest germination inhibition was achieved by the sorghum variety Mandau with watering every 5 days”

✓ located verbatim in the source · abstract, characters 1278–1382 · show in text ↑
e1

“25 mung bean seeds were planted”

✓ located verbatim in the source · abstract, characters 978–1009 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Authors consider the strongest inhibitory treatment a potential bioherbicide.

Taxon
mung beanVigna radiatae1
Outcome
potential to be the best bioherbicidee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The treatment produced the highest inhibition on the test plants, so it has the potential to be the best bioherbicide”

✓ located verbatim in the source · abstract, characters 1384–1501 · show in text ↑
e1

“25 mung bean seeds were planted”

✓ located verbatim in the source · abstract, characters 978–1009 · show in text ↑

irrigation · disease · Vigna radiata (mung bean)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.