Abstract
Purpose: to determine the total flavonoid content and seed germination characteristics in bioassay tests due to sorghum extracts' application at various drought stress levels. Extract materials were prepared from June to August 2024 at Bengkulu University Greenhouse, Kandang Limun Village, Bengkulu City, and bioassay tests were conducted in August 2024 at Pematang Gubernur, Muara Bangkahulu, Bengkulu City. Flavonoid analysis was performed at the Integrated Research and Testing Laboratory (LPPT) of Gadjah Mada University in August 2024. Methodology: This study used a completely randomized design (CRD) with one factor. Two sorghum varieties, Super 2 and Mandau, were planted with water stress treatment through watering patterns every day, every 3 days, and every 5 days. The experimental unit was a petri dish, and the experiment was repeated four times. The Petri dish bioassay method was applied in this experiment.Each petri dish was given 10 mL of water extract, and 25 mung bean seeds were planted and incubated for three days. Results: The results showed that the Mandau variety had the highest total flavonoid content with watering every 3 days (3991.34 µg/g), and the Super 2 variety had the lowest total flavonoid content with watering every day (3165.26 µg/g). The highest germination inhibition was achieved by the sorghum variety Mandau with watering every 5 days. The treatment produced the highest inhibition on the test plants, so it has the potential to be the best bioherbicide. Findings: The Mandau variety has the highest total flavonoid content with watering every 3 days, and the Super 2 variety has the lowest total flavonoid content with watering every day. Novelty: This study provides insight into the total flavonoid content and seed germination characteristics due to the application of sorghum extracts at various levels of drought stress. Originality: This study offers a detailed analysis of total flavonoid levels and seed germination characteristics in bioassay tests. Conclusion: The Mandau variety had the highest total flavonoid content with watering every 3 days. The Mandau sorghum variety achieved the highest germination inhibition with watering every 5 days. Type of Paper: empirical research article.
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Claims 4
Claim · model-written summary
Mandau under watering every 3 days had the highest flavonoid content, 3991.34 µg/g.
- Material
- Mandaue0
- Treatment
- watering every 3 dayse0
- Outcome
- total flavonoid contente0
- Direction
- higheste0
- Value
- 3991.34 µg/ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the Mandau variety had the highest total flavonoid content with watering every 3 days (3991.34 µg/g)”
t0“Sorghum”
Claim · model-written summary
Super 2 under daily watering had the lowest flavonoid content, 3165.26 µg/g.
- Material
- Super 2e0
- Treatment
- watering every daye0
- Outcome
- total flavonoid contente0
- Direction
- loweste0
- Value
- 3165.26 µg/ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the Super 2 variety had the lowest total flavonoid content with watering every day (3165.26 µg/g)”
t0“Sorghum”
Claim · model-written summary
Mandau extract from five day watering had the highest mung bean germination inhibition.
- Taxon
- mung bean
Vigna radiatae1 - Treatment
- sorghum variety Mandau with watering every 5 dayse0
- Outcome
- germination inhibitione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)

Verbatim
e0“The highest germination inhibition was achieved by the sorghum variety Mandau with watering every 5 days”
e1“25 mung bean seeds were planted”
Claim · model-written summary
Authors consider the strongest inhibitory treatment a potential bioherbicide.
- Taxon
- mung bean
Vigna radiatae1 - Outcome
- potential to be the best bioherbicidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The treatment produced the highest inhibition on the test plants, so it has the potential to be the best bioherbicide”
e1“25 mung bean seeds were planted”
The same questions in other papers
irrigation · disease · Vigna radiata (mung bean)Positive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.