Paper · 2025 · ACS Omega

Identification and Characterization of Organic Calcium Crystals in the Plumeria pudica and Plumeria rubra Latexes

Larissa Barbosa Nogueira Freitas; Cleverson Diniz Teixeira de Freitas; Alejandro Pedro Ayala; et al.

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verbatim quotes
0
citations
2
research areas
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Open access · cc-by

Abstract

High Resolution Image Download MS PowerPoint Slide Latex is usually a milky fluid but can also be clear, brown, or orange. It consists of organic compounds (rubber particles and primary and secondary metabolites) and proteins dispersed in an aqueous phase. Four latex fluids were examined through optical microscopy, and organic crystals were found. Crystal structures were observed in Plumeria rubra and P. pudica, while no crystal was found in the latex of Calotropis procera and Himatanthus drasticus . Various sizes and shapes of crystals, including raphides, styloids, crystalline sands, and prismatic forms, were documented. The structures were stable for a 96 h period at 25 °C. The relative abundance of crystal forms varied based on whether the latex was crude or water-diluted. All crystal forms related to P. pudica were identified as hydrated calcium malonate [Ca(C 3 H 2 O 4 )(H 2 O) 2 ]·2H 2 O, representing the first documentation of these structures in plants. Calcium malonate crystals also were identified in the latex of P. rubra, which were mainly in the forms of raphides and styloids. Nonetheless, the crystalline sands and prismatic forms found in P. rubra latex exhibited an additional carbon ring in their structure ([C 20 H 34 Ca 2 O 19 ]) identified as cerberic acid B. This result also represents the first report of calcium-containing cerberic acid B. The results were discussed based on the possible functions of these molecules within laticifers.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Crystals were observed in Plumeria rubra latex.

Taxon
Plumeria rubraPlumeria rubrae0
Outcome
Crystal structurese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria rubra
Plumeria rubra · TaxonZachi Evenor and MathKnight · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Crystal structures were observed in Plumeria rubra and P. pudica”

✓ located verbatim in the source · abstract, characters 350–414 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Crystals were observed in P. pudica latex.

Taxon
P. pudicaPlumeria pudicae0
Outcome
Crystal structurese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria pudica
Plumeria pudica · TaxonForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Crystal structures were observed in Plumeria rubra and P. pudica”

✓ located verbatim in the source · abstract, characters 350–414 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No crystals were found in Calotropis procera latex.

Taxon
Calotropis proceraCalotropis procerae0
Outcome
no crystal was founde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Calotropis procera
Calotropis procera · TaxonWilfredo Rodríguez · CC0 · Wikimedia Commons ↗

Verbatim

e0

“no crystal was found in the latex of Calotropis procera and Himatanthus drasticus”

✓ located verbatim in the source · abstract, characters 422–503 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No crystals were found in Himatanthus drasticus latex.

Taxon
Himatanthus drasticusHimatanthus drasticuse0
Outcome
no crystal was founde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Himatanthus drasticus
Himatanthus drasticus · TaxonCostaPPPR · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“no crystal was found in the latex of Calotropis procera and Himatanthus drasticus”

✓ located verbatim in the source · abstract, characters 422–503 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Plumeria latex crystals included raphides, styloids, crystalline sands and prismatic forms.

Taxon
Plumeria pudica and Plumeria rubraPlumeria rubraPlumeria pudicat0
Outcome
raphides, styloids, crystalline sands, and prismatic formse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria rubra
Plumeria rubra · TaxonZachi Evenor and MathKnight · CC BY 2.0 · Wikimedia Commons ↗
Plumeria pudica
Plumeria pudica · TaxonForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Various sizes and shapes of crystals, including raphides, styloids, crystalline sands, and prismatic forms, were documented”

✓ located verbatim in the source · abstract, characters 506–629 · show in text ↑
t0

“Plumeria pudica and Plumeria rubra”

✓ located verbatim in the source · title, characters 71–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Crystal structures remained stable for 96 hours at 25 °C.

Taxon
Plumeria pudica and Plumeria rubraPlumeria rubraPlumeria pudicat0
Outcome
structures were stablee0
Value
96 he0
Qualifier
25 °Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria rubra
Plumeria rubra · TaxonZachi Evenor and MathKnight · CC BY 2.0 · Wikimedia Commons ↗
Plumeria pudica
Plumeria pudica · TaxonForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The structures were stable for a 96 h period at 25 °C”

✓ located verbatim in the source · abstract, characters 631–684 · show in text ↑
t0

“Plumeria pudica and Plumeria rubra”

✓ located verbatim in the source · title, characters 71–105 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Crystal form abundance varied between crude and diluted latex.

Taxon
Plumeria pudica and Plumeria rubraPlumeria rubraPlumeria pudicat0
Treatment
crude or water-dilutede0
Outcome
relative abundance of crystal formse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria rubra
Plumeria rubra · TaxonZachi Evenor and MathKnight · CC BY 2.0 · Wikimedia Commons ↗
Plumeria pudica
Plumeria pudica · TaxonForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The relative abundance of crystal forms varied based on whether the latex was crude or water-diluted”

✓ located verbatim in the source · abstract, characters 686–786 · show in text ↑
t0

“Plumeria pudica and Plumeria rubra”

✓ located verbatim in the source · title, characters 71–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

All observed P. pudica crystal forms were hydrated calcium malonate.

Taxon
P. pudicaPlumeria pudicae0
Outcome
hydrated calcium malonatee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria pudica
Plumeria pudica · TaxonForest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“All crystal forms related to P. pudica were identified as hydrated calcium malonate [Ca(C 3 H 2 O 4 )(H 2 O) 2 ]·2H 2 O”

✓ located verbatim in the source · abstract, characters 788–907 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

P. rubra latex contained calcium malonate crystals, mainly raphides and styloids.

Taxon
P. rubraPlumeria rubrae0
Outcome
Calcium malonate crystalse0
Qualifier
raphides and styloidse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria rubra
Plumeria rubra · TaxonZachi Evenor and MathKnight · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Calcium malonate crystals also were identified in the latex of P. rubra, which were mainly in the forms of raphides and styloids”

✓ located verbatim in the source · abstract, characters 977–1105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

P. rubra crystalline sands and prisms contained cerberic acid B.

Taxon
P. rubraPlumeria rubrae0
Organ / stage
latexe0
Outcome
cerberic acid Be0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Plumeria rubra
Plumeria rubra · TaxonZachi Evenor and MathKnight · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the crystalline sands and prismatic forms found in P. rubra latex exhibited an additional carbon ring in their structure ([C 20 H 34 Ca 2 O 19 ]) identified as cerberic acid B”

✓ located verbatim in the source · abstract, characters 1120–1295 · show in text ↑