Paper · 2025 · Scientific Reports

Potential effect of phyto-synthesized silver nanoparticles using Cotula cinerea Del Raw extract on salt tolerance of wheat seeds (Triticum durum desf, Boussellam variety) germination

Brahim Kesbi; Nasrine Salhi; Yasmina Khane; et al.

11
claims
23
verbatim quotes
9
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

This study uncovers a novel and environmentally sustainable method for synthesizing silver nanoparticles (AgNPs) utilizing Cotula cinerea extract, a plant that has not been previously explored for nanoparticle (NP) fabrication. The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety). AgNPs extract spectra showed a sharp peak at 445.91 nm in UV-vis while X-ray diffraction (XRD) reflected the patterns of the face-centred cubic (fcc) and crystalline structure and the scanning electron microscopy (SEM) study revealed that the NPs have an almost spherical and somewhat cuboidal morphology, with dimensions under 20 nm. The transmission electron microscopy (TEM) study revealed an average particle size distribution of 15.128 nm, largely exhibiting a spherical morphology. The hydrodynamic diameter measured (DLS) indicated a particle size of 351.6 nm, which is much larger. Seeds were treated with 0, 20, 40, and 80 mg L-1 of AgNPs and exposed to 0 and 150 mM NaCl. At a concentration of 40 mg/L of AgNPs, germinability attained 90% under saline conditions, in contrast to 70% for the control group, while root length (RL) exhibited an 86% increase, measuring 7.28 cm compared to 3.9 cm in the control group. At a concentration of 20 mg/L of AgNPs, root fresh weight (RFW) increases from 0.04 to 0.06 g in saline conditions and from 0.06 to 0.09 g in the absence of salt. The control group exhibited a root number (RN) of 3.67, while plants treated with 20 mg/L demonstrated a significant increase to 5.54. Additionally, shoot length (SL) under saline stress reached 11.12 cm, compared to 8.26 cm in saline conditions. These findings underscore the potential of green synthesised AgNPs as an effective approach to enhance the salt tolerance of durum wheat and promote sustainable agriculture in saline conditions.

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Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNPs at 40 mg/L increased germinability under saline conditions to 90%, compared with 70% in the control group.

Treatment
40 mg/L of AgNPse1
Outcome
germinabilitye1
Direction
increaseinferrede1
Value
90%e1
Qualifier
70%e1
Comparator
control groupe1
Setting
saline conditionse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum turgidum subsp. durum
Triticum turgidum subsp. durum · TaxonSten Porse · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety)”

✓ located verbatim in the source · abstract, characters 228–363 · show in text ↑
e1

“At a concentration of 40 mg/L of AgNPs, germinability attained 90% under saline conditions, in contrast to 70% for the control group”

✓ located verbatim in the source · abstract, characters 1047–1179 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNPs at 40 mg/L increased root length by 86%, to 7.28 cm compared with 3.9 cm in the control group.

Treatment
40 mg/L of AgNPse1
Outcome
root length (RL)e2
Direction
increasee2
Value
86%e2
Qualifier
3.9 cme2
Comparator
control groupe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum turgidum subsp. durum
Triticum turgidum subsp. durum · TaxonSten Porse · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety)”

✓ located verbatim in the source · abstract, characters 228–363 · show in text ↑
e1

“At a concentration of 40 mg/L of AgNPs, germinability attained 90% under saline conditions, in contrast to 70% for the control group”

✓ located verbatim in the source · abstract, characters 1047–1179 · show in text ↑
e2

“while root length (RL) exhibited an 86% increase, measuring 7.28 cm compared to 3.9 cm in the control group”

✓ located verbatim in the source · abstract, characters 1181–1288 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNPs at 20 mg/L increased root fresh weight under saline conditions from 0.04 to 0.06 g.

Treatment
20 mg/L of AgNPse1
Outcome
root fresh weighte1
Direction
increasese1
Value
0.04 to 0.06 ge1
Setting
saline conditionse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum turgidum subsp. durum
Triticum turgidum subsp. durum · TaxonSten Porse · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety)”

✓ located verbatim in the source · abstract, characters 228–363 · show in text ↑
e1

“At a concentration of 20 mg/L of AgNPs, root fresh weight (RFW) increases from 0.04 to 0.06 g in saline conditions and from 0.06 to 0.09 g in the absence of salt”

✓ located verbatim in the source · abstract, characters 1290–1451 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNPs at 20 mg/L increased root fresh weight in the absence of salt from 0.06 to 0.09 g.

Treatment
20 mg/L of AgNPse1
Outcome
root fresh weighte1
Direction
increasese1
Value
0.06 to 0.09 ge1
Setting
absence of salte1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum turgidum subsp. durum
Triticum turgidum subsp. durum · TaxonSten Porse · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety)”

✓ located verbatim in the source · abstract, characters 228–363 · show in text ↑
e1

“At a concentration of 20 mg/L of AgNPs, root fresh weight (RFW) increases from 0.04 to 0.06 g in saline conditions and from 0.06 to 0.09 g in the absence of salt”

✓ located verbatim in the source · abstract, characters 1290–1451 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

AgNPs at 20 mg/L significantly increased root number to 5.54, compared with 3.67 in the control group.

Treatment
20 mg/Le1
Outcome
root number (RN)e1
Direction
increasee1
Value
5.54e1
Qualifier
3.67e1
Comparator
control groupe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum turgidum subsp. durum
Triticum turgidum subsp. durum · TaxonSten Porse · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety)”

✓ located verbatim in the source · abstract, characters 228–363 · show in text ↑
e1

“The control group exhibited a root number (RN) of 3.67, while plants treated with 20 mg/L demonstrated a significant increase to 5.54”

✓ located verbatim in the source · abstract, characters 1453–1586 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under saline stress, shoot length reached 11.12 cm, versus 8.26 cm for the saline-conditions comparison as written.

Treatment
saline stresse1
Outcome
shoot length (SL)e1
Direction
increaseinferrede1
Value
11.12 cme1
Qualifier
8.26 cme1
Comparator
saline conditionse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum turgidum subsp. durum
Triticum turgidum subsp. durum · TaxonSten Porse · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study is to evaluate the efficacy of a nano primer in mitigating salt stress in durum wheat (Triticum durum Desf Boussalem variety)”

✓ located verbatim in the source · abstract, characters 228–363 · show in text ↑
e1

“Additionally, shoot length (SL) under saline stress reached 11.12 cm, compared to 8.26 cm in saline conditions”

✓ located verbatim in the source · abstract, characters 1588–1698 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

AgNPs made with Cotula cinerea extract show a sharp UV-vis peak at 445.91 nm.

Taxon
Cotula cinereaCotula cinereae0
Outcome
sharp peake1
Value
445.91 nme1

Verbatim

e0

“utilizing Cotula cinerea extract”

✓ located verbatim in the source · abstract, characters 113–145 · show in text ↑
e1

“AgNPs extract spectra showed a sharp peak at 445.91 nm in UV-vis”

✓ located verbatim in the source · abstract, characters 365–429 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

XRD of the Cotula cinerea AgNPs reflected a face-centred cubic and crystalline structure.

Taxon
Cotula cinereaCotula cinereae0
Outcome
patterns of the face-centred cubic (fcc) and crystalline structuree1

Verbatim

e0

“utilizing Cotula cinerea extract”

✓ located verbatim in the source · abstract, characters 113–145 · show in text ↑
e1

“X-ray diffraction (XRD) reflected the patterns of the face-centred cubic (fcc) and crystalline structure”

✓ located verbatim in the source · abstract, characters 436–540 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

SEM shows Cotula cinerea AgNPs with almost spherical and somewhat cuboidal morphology and dimensions under 20 nm.

Taxon
Cotula cinereaCotula cinereae0
Outcome
morphologye1
Value
under 20 nme1
Qualifier
almost spherical and somewhat cuboidale1

Verbatim

e0

“utilizing Cotula cinerea extract”

✓ located verbatim in the source · abstract, characters 113–145 · show in text ↑
e1

“the scanning electron microscopy (SEM) study revealed that the NPs have an almost spherical and somewhat cuboidal morphology, with dimensions under 20 nm”

✓ located verbatim in the source · abstract, characters 545–698 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

TEM gives an average particle size of 15.128 nm for the Cotula cinerea AgNPs.

Taxon
Cotula cinereaCotula cinereae0
Outcome
average particle size distributione1
Value
15.128 nme1

Verbatim

e0

“utilizing Cotula cinerea extract”

✓ located verbatim in the source · abstract, characters 113–145 · show in text ↑
e1

“The transmission electron microscopy (TEM) study revealed an average particle size distribution of 15.128 nm”

✓ located verbatim in the source · abstract, characters 700–808 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

DLS gives a hydrodynamic diameter of 351.6 nm for the Cotula cinerea AgNPs.

Taxon
Cotula cinereaCotula cinereae0
Outcome
hydrodynamic diametere1
Value
351.6 nme1

Verbatim

e0

“utilizing Cotula cinerea extract”

✓ located verbatim in the source · abstract, characters 113–145 · show in text ↑
e1

“The hydrodynamic diameter measured (DLS) indicated a particle size of 351.6 nm”

✓ located verbatim in the source · abstract, characters 853–931 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

silver nanoparticle · growth · Triticum turgidum (rivet wheat)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

salinity · growth · Triticum turgidum (rivet wheat)Positive and negative results

29 papers: 4 positive, 25 negative or no effect

3 papers in the same direction as this paper · 25 in the opposite direction

  1. negative
  2. negative
  3. negative

25 papers more in this group

See all 29 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.