Abstract
Abstract In the present study, a novel CYP86A22-like (CYP86A22L) was identified from Citrus maxima fruits. CmCYP86a22L contained most conserved motifs of plant CYP450 family, and its transcript levels was high in different fruit tissues but dramatically decreased during fruit development. To elucidate the specific role of the variable substrate recognition site (SRS) SRS6 region in CmCYP86A22L, C-terminal fragment containing SRS6 region was transiently expressed in tobacco leaves, and exogenous CmCYP86A22L mRNAs were significantly upregulated as expected. Furthermore, the stem diameters of CmCYP86A22L transient expression lines were wider than mock in 3~12 days after infiltration, accompanied by increases cell layers of xylem, phloem and cortex, and cortex cell volume. Additionally, the CmCYP86A22L transient expression stems lacked visible cuticle on epidermal cells. These results collectively suggested that CmCYP86A22L was involved in fruit development, and the SRS6 region of CmCYP86a22L could regulate stem growth.
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Claims 11
Claim · model-written summary
A novel CYP86A22-like gene was identified in Citrus maxima fruit.
- Taxon
- Citrus maxima
Citrus maximae0 - Organ / stage
- fruitse0
- Gene
- CYP86A22-like (CYP86A22L)e0
- Outcome
- identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel CYP86A22-like (CYP86A22L) was identified from Citrus maxima fruits.”
Claim · model-written summary
CmCYP86a22L contained most conserved motifs of the plant CYP450 family.
- Taxon
- Citrus maximaCitrus maximat0
- Gene
- CmCYP86a22Le0
- Outcome
- most conserved motifs of plant CYP450 familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmCYP86a22L contained most conserved motifs of plant CYP450 family”
t0“Citrus maxima”
Claim · model-written summary
CmCYP86a22L transcript levels were high in different fruit tissues.
- Taxon
- Citrus maximaCitrus maximat0
- Organ / stage
- fruit tissuese0
- Gene
- CYP86A22-liket0
- Outcome
- transcript levelse0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its transcript levels was high in different fruit tissues”
t0“Citrus maxima CYP86A22-like”
Claim · model-written summary
CmCYP86a22L transcript levels decreased dramatically during fruit development.
- Taxon
- Citrus maximaCitrus maximat0
- Organ / stage
- fruit developmente0
- Gene
- CYP86A22-liket0
- Outcome
- transcript levelse1
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dramatically decreased during fruit development.”
t0“Citrus maxima CYP86A22-like”
e1“its transcript levels was high in different fruit tissues”
Claim · model-written summary
Transient expression in tobacco leaves significantly upregulated exogenous CmCYP86A22L mRNAs.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leavese0
- Treatment
- C-terminal fragment containing SRS6 region was transiently expressede0
- Gene
- CmCYP86A22Le0
- Outcome
- exogenous CmCYP86A22L mRNAse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C-terminal fragment containing SRS6 region was transiently expressed in tobacco leaves, and exogenous CmCYP86A22L mRNAs were significantly upregulated as expected.”
Claim · model-written summary
CmCYP86A22L expression lines had wider stems than mock lines 3–12 days after infiltration.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- CmCYP86A22L transient expressione0
- Gene
- CmCYP86A22Le0
- Outcome
- stem diameterse0
- Direction
- widere0
- Qualifier
- 3~12 days after infiltratione0
- Comparator
- mocke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stem diameters of CmCYP86A22L transient expression lines were wider than mock in 3~12 days after infiltration”
e1“tobacco leaves, and exogenous CmCYP86A22L mRNAs”
Claim · model-written summary
CmCYP86A22L expression increased xylem, phloem and cortex cell layers.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- CmCYP86A22L transient expressione2
- Outcome
- cell layers of xylem, phloem and cortexe0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accompanied by increases cell layers of xylem, phloem and cortex, and cortex cell volume.”
e1“tobacco leaves, and exogenous CmCYP86A22L mRNAs”
e2“the stem diameters of CmCYP86A22L transient expression lines”
Claim · model-written summary
CmCYP86A22L expression increased cortex cell volume.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- CmCYP86A22L transient expressione2
- Outcome
- cortex cell volumee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increases cell layers of xylem, phloem and cortex, and cortex cell volume.”
e1“tobacco leaves, and exogenous CmCYP86A22L mRNAs”
e2“the stem diameters of CmCYP86A22L transient expression lines”
Claim · model-written summary
Transient expression stems lacked visible epidermal cuticle.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- CmCYP86A22L transient expressione0
- Outcome
- visible cuticle on epidermal cellse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CmCYP86A22L transient expression stems lacked visible cuticle on epidermal cells.”
e1“tobacco leaves, and exogenous CmCYP86A22L mRNAs”
Claim · model-written summary
Authors inferred CmCYP86A22L involvement in fruit development.
- Taxon
- Citrus maximaCitrus maximat0
- Gene
- CmCYP86A22Le0
- Outcome
- fruit developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmCYP86A22L was involved in fruit development”
t0“Citrus maxima”
Claim · model-written summary
Authors inferred that the CmCYP86a22L SRS6 region regulates stem growth.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- CmCYP86a22Le0
- Outcome
- stem growthe0
- Qualifier
- SRS6 regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SRS6 region of CmCYP86a22L could regulate stem growth.”
e1“tobacco leaves, and exogenous CmCYP86A22L mRNAs”