Abstract
This study established an efficient in vitro regeneration system for Tibouchina granulosato (Desr.) Cogn. petiolated leaves to address the low propagation efficiency and propagatable germplasm shortages. The results revealed that the Murashige and Skoog (MS) medium supplemented with 1.1 mg/L of Zeatin (ZT) and 0.2 mg/L of 1-naphthyl acetic acid (NAA) was the optimal formulation for callus induction, yielding callus induction of 89.59%. For adventitious bud induction, the combination of 2.0 mg/L of 6-benzyladenine (BA) and 0.4 mg/L of NAA proved most effective, achieving an induction rate of 83.33%. Additionally, the adventitious shoots exhibited remarkable elongation when cultured in a medium containing 0.2 mg/L of BA and 0.04 mg/L of NAA. All explants rooted when treated with 0.5 mg/l NAA, inducing a mean number of 6.90 roots per plant and a survival percentage of 91.00%. This study provided technical support for the promotion of superior varieties and genetic improvement of Tibouchina granulosa.
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Claims 6
Claim · model-written summary
Zeatin and NAA medium gave optimal callus induction of 89.59%.
- Taxon
- Tibouchina granulosa
Pleroma granulosumt0 - Treatment
- 1.1 mg/L of Zeatin (ZT) and 0.2 mg/L of 1-naphthyl acetic acid (NAA)e0
- Outcome
- callus inductione0
- Direction
- optimale0
- Value
- 89.59%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Murashige and Skoog (MS) medium supplemented with 1.1 mg/L of Zeatin (ZT) and 0.2 mg/L of 1-naphthyl acetic acid (NAA) was the optimal formulation for callus induction, yielding callus induction of 89.59%”
t0“Tibouchina granulosa”
Claim · model-written summary
BA and NAA medium gave an 83.33% adventitious bud induction rate.
- Taxon
- Tibouchina granulosaPleroma granulosumt0
- Treatment
- 2.0 mg/L of 6-benzyladenine (BA) and 0.4 mg/L of NAAe0
- Outcome
- induction ratee0
- Direction
- most effectivee0
- Value
- 83.33%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination of 2.0 mg/L of 6-benzyladenine (BA) and 0.4 mg/L of NAA proved most effective, achieving an induction rate of 83.33%”
t0“Tibouchina granulosa”
Claim · model-written summary
Shoots elongated remarkably in medium with 0.2 mg/L BA and 0.04 mg/L NAA.
- Taxon
- Tibouchina granulosaPleroma granulosumt0
- Organ / stage
- adventitious shootse0
- Treatment
- 0.2 mg/L of BA and 0.04 mg/L of NAAe0
- Outcome
- elongatione0
- Direction
- remarkablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the adventitious shoots exhibited remarkable elongation when cultured in a medium containing 0.2 mg/L of BA and 0.04 mg/L of NAA”
t0“Tibouchina granulosa”
Claim · model-written summary
All explants rooted under 0.5 mg/l NAA.
- Taxon
- Tibouchina granulosaPleroma granulosumt0
- Treatment
- 0.5 mg/l NAAe0
- Outcome
- rootede0
- Direction
- Alle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All explants rooted when treated with 0.5 mg/l NAA, inducing a mean number of 6.90 roots per plant and a survival percentage of 91.00%”
t0“Tibouchina granulosa”
Claim · model-written summary
The 0.5 mg/l NAA treatment induced a mean 6.90 roots per plant.
- Taxon
- Tibouchina granulosaPleroma granulosumt0
- Treatment
- 0.5 mg/l NAAe0
- Outcome
- roots per plante0
- Direction
- inducinge0
- Value
- 6.90 roots per plante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All explants rooted when treated with 0.5 mg/l NAA, inducing a mean number of 6.90 roots per plant and a survival percentage of 91.00%”
t0“Tibouchina granulosa”
Claim · model-written summary
The 0.5 mg/l NAA treatment produced 91.00% survival.
- Taxon
- Tibouchina granulosaPleroma granulosumt0
- Treatment
- 0.5 mg/l NAAe0
- Outcome
- survival percentagee0
- Value
- 91.00%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All explants rooted when treated with 0.5 mg/l NAA, inducing a mean number of 6.90 roots per plant and a survival percentage of 91.00%”
t0“Tibouchina granulosa”