Abstract
Somatic embryogenesis (SE) is a crucial strategy for in vitro regeneration in woody plants, yet its efficiency is affected by multiple factors, and the underlying regulatory mechanisms remain insufficiently understood. In this study, callus tissues from two Liriodendron sino-americanum genotypes involving different hybrid combinations, ON-LoS and TN-LoS, were treated with varying concentrations (0, 0.005, 0.01, 0.05 mg/L) of exogenous zeatin (ZT) to evaluate its regulatory effect on SE. Treatment with 0.01 mg/L ZT significantly promoted SE in ON-LoS but suppressed it in TN-LoS, indicating that ZT elicited divergent regulatory effects on SE between the two genotypes. To explore the molecular basis of this divergence, transcriptome analysis was conducted at the early stage of SE. Differentially expressed genes (DEGs) were significantly enriched in hormone signaling, particularly in the cytokinin (CK) and brassinosteroid (BR) signaling pathways, as well as biosynthetic and redox-related pathways. In particular, given the established role of cell cycle-related gene CYCD3 (Lchi20922) in promoting cell division, CYCD3 was markedly upregulated by ZT in ON-LoS but downregulated in TN-LoS. These results indicate that ZT regulates SE efficiency through differential modulation of CYCD3 expression in distinct genotypes. This study enhances our understanding of the molecular basis of SE regulation in Liriodendron sino-americanum and offers a theoretical framework for improving regeneration efficiency in woody plants.
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Claims 6
Claim · model-written summary
Zeatin at 0.01 mg/L significantly promoted somatic embryogenesis in ON-LoS.
- Taxon
- Liriodendron sino-americanume1
- Material
- ON-LoSe0
- Treatment
- 0.01 mg/L ZTe0
- Outcome
- SEe0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“Treatment with 0.01 mg/L ZT significantly promoted SE in ON-LoS”
e1“Liriodendron sino-americanum genotypes”
Claim · model-written summary
Zeatin at 0.01 mg/L suppressed somatic embryogenesis in TN-LoS.
- Taxon
- Liriodendron sino-americanume1
- Material
- TN-LoSe0
- Treatment
- 0.01 mg/L ZTe0
- Outcome
- SEe0
- Direction
- suppressede0
Not reportedComparator
Verbatim
e0“Treatment with 0.01 mg/L ZT significantly promoted SE in ON-LoS but suppressed it in TN-LoS”
e1“Liriodendron sino-americanum genotypes”
Claim · model-written summary
Differentially expressed genes were enriched in hormone signaling and biosynthetic and redox pathways.
- Taxon
- Liriodendron sino-americanume1
- Outcome
- Differentially expressed genes (DEGs)e0
- Qualifier
- hormone signalinge0
Not reportedDirection
Verbatim
e0“Differentially expressed genes (DEGs) were significantly enriched in hormone signaling, particularly in the cytokinin (CK) and brassinosteroid (BR) signaling pathways, as well as biosynthetic and redox-related pathways.”
e1“Liriodendron sino-americanum genotypes”
Claim · model-written summary
Zeatin upregulated CYCD3 in ON-LoS.
- Taxon
- Liriodendron sino-americanume1
- Material
- ON-LoSe0
- Treatment
- ZTe0
- Gene
- CYCD3e0
- Outcome
- CYCD3e0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
e0“CYCD3 was markedly upregulated by ZT in ON-LoS”
e1“Liriodendron sino-americanum genotypes”
Claim · model-written summary
Zeatin downregulated CYCD3 in TN-LoS.
- Taxon
- Liriodendron sino-americanume1
- Material
- TN-LoSe0
- Treatment
- ZTe0
- Gene
- CYCD3e0
- Outcome
- CYCD3e0
- Direction
- downregulatede0
Not reportedComparator
Verbatim
e0“CYCD3 was markedly upregulated by ZT in ON-LoS but downregulated in TN-LoS”
e1“Liriodendron sino-americanum genotypes”
Claim · model-written summary
Authors infer that zeatin regulates somatic embryogenesis efficiency through differential CYCD3 expression.
Verbatim
e0“ZT regulates SE efficiency through differential modulation of CYCD3 expression in distinct genotypes”
e1“SE regulation in Liriodendron sino-americanum”