Abstract
The article presents the results of extraction of butanol extract from the aboveground part of the family Asteraceae of genus Symphyotrichum novi-belgii, growing in the East Kazakhstan region in September 2021, using silica gel as an effective sorbent in column chromatography and separation of quercetin from the flavonoid fraction. The purpose of the work is to study the chemical composition of the plant of the genus Symphyotricum novi-belgii, to develop a method for obtaining biologically active substances and to establish the structure of individual substances by using modern physical and chemical methods. The study revealed the quantitative content of biologically active substances in the composition of a plant of the genus Symphyotrichum novi-belgii, as a result of which flavonoids amounted to 1.45±0.03 %, tannins 1.30±0.03%, organic acids 1.43±0.02%, saponins 1.05±0.04%. Extraction of the flavonoid complex from the plant Symphyotrichum novi-belgii It was carried out with effective extraction modes (maceration with 80% ethanol, 72 hours, hydromodule 1:8). 47 mg fractions 17-19 were selected from of the flavonoid complex by column chromatography on silica gel and qualitative analysis was performed using cerium (IV) sulfate Ce(SO4)2 as an eluent in thin-layer chromatography. As a result, the structure of the isolated individual compound was confirmed by modern methods of 1H-NMR, 13C-NMR and mass spectrometry (EI-MS). As a result, the individual substance was found to be quercetin (2.1 mg).
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Claims 6
Claim · model-written summary
The aboveground part of Symphyotrichum novi-belgii contained flavonoids at 1.45±0.03 %.
- Taxon
- Symphyotrichum novi-belgii
.jpg?width=60)
Symphyotrichum novi-belgiit0 - Outcome
- flavonoidse0
- Value
- 1.45±0.03 %e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“SYMPHYOTRICHUM NOVI-BELGII”
e0“flavonoids amounted to 1.45±0.03 %”
Claim · model-written summary
The aboveground part of Symphyotrichum novi-belgii contained tannins at 1.30±0.03%.
- Taxon
- Symphyotrichum novi-belgiiSymphyotrichum novi-belgiit0
- Outcome
- tanninse0
- Value
- 1.30±0.03%e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SYMPHYOTRICHUM NOVI-BELGII”
e0“tannins 1.30±0.03%”
Claim · model-written summary
The aboveground part of Symphyotrichum novi-belgii contained organic acids at 1.43±0.02%.
- Taxon
- Symphyotrichum novi-belgiiSymphyotrichum novi-belgiit0
- Outcome
- organic acidse0
- Value
- 1.43±0.02%e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SYMPHYOTRICHUM NOVI-BELGII”
e0“organic acids 1.43±0.02%”
Claim · model-written summary
The aboveground part of Symphyotrichum novi-belgii contained saponins at 1.05±0.04%.
- Taxon
- Symphyotrichum novi-belgiiSymphyotrichum novi-belgiit0
- Outcome
- saponinse0
- Value
- 1.05±0.04%e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SYMPHYOTRICHUM NOVI-BELGII”
e0“saponins 1.05±0.04%”
Claim · model-written summary
Flavonoid complex extraction from Symphyotrichum novi-belgii used maceration with 80% ethanol, 72 hours, hydromodule 1:8 as effective extraction modes.
Not reportedTaxonTreatmentComparatorDirection
Verbatim
e0“It was carried out with effective extraction modes (maceration with 80% ethanol, 72 hours, hydromodule 1:8)”
Claim · model-written summary
The individual substance isolated from the flavonoid complex of Symphyotrichum novi-belgii was identified as quercetin (2.1 mg), with structure confirmed by NMR and mass spectrometry.
- Taxon
- Symphyotrichum novi-belgiiSymphyotrichum novi-belgiit0
- Outcome
- individual substance was found to be quercetine0
- Value
- 2.1 mge0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SYMPHYOTRICHUM NOVI-BELGII”
e0“the individual substance was found to be quercetin (2.1 mg)”