Abstract
The secondary cell wall (SCW) is a critical supportive and protective structure in plant growth and development. In cotton fibers, it is established that SCW thickening directly determines fiber strength by enhancing mechanical support, but the molecular mechanisms governing this process remain poorly understood. Here, we elucidate a ubiquitin-GhUBX-GhCDC48 regulatory module in which GhUBX serves as a bridge between GhCDC48 and ubiquitin, promoting GhCDC48 degradation via the ubiquitin-26S proteasome pathway. Compared to the wild type receptor (W0), transgenic cotton lines in which GhUBX is silenced or GhCDC48 is overexpressed exhibited significantly increased SCW thickness and fiber strength. Conversely, GhCDC48 silencing resulted in stunted plant growth and impaired cell wall development, consistent with its role in regulating the cell division cycle. Furthermore, we revealed that GhCDC48 abundance positively correlates with ATPase activity in fiber cells and with the transcriptional activation of key SCW biosynthesis genes including GhCesA7, GhCesA8, GhSusA, GhFSN1, GhKNL1, and GhNAC21, thereby enhancing SCW deposition. Our findings reveal the ubiquitin-GhUBX-CDC48 regulatory module that influences cotton fiber strength and provide a genetic strategy for improving fiber quality through targeted modification of genes in the ubiquitin pathway.
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Claims 7
Claim · model-written summary
GhUBX bridges GhCDC48 and ubiquitin, promoting proteasomal degradation of GhCDC48.
- Taxon
- cotton
Gossypium hirsutumt0 - Gene
- GhUBXe0
- Outcome
- GhCDC48 degradatione0
- Qualifier
- ubiquitin-26S proteasome pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhUBX serves as a bridge between GhCDC48 and ubiquitin, promoting GhCDC48 degradation via the ubiquitin-26S proteasome pathway”
t0“cotton fiber”
Claim · model-written summary
GhUBX silencing significantly increased cotton fiber secondary cell wall thickness and strength versus wild type.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GhUBX is silencede0
- Gene
- GhUBXe0
- Outcome
- SCW thickness and fiber strengthe0
- Direction
- increasede0
- Comparator
- wild type receptor (W0)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the wild type receptor (W0), transgenic cotton lines in which GhUBX is silenced or GhCDC48 is overexpressed exhibited significantly increased SCW thickness and fiber strength”
Claim · model-written summary
GhCDC48 overexpression significantly increased cotton fiber secondary cell wall thickness and strength versus wild type.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GhCDC48 is overexpressede0
- Gene
- GhCDC48e0
- Outcome
- SCW thickness and fiber strengthe0
- Direction
- increasede0
- Comparator
- wild type receptor (W0)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the wild type receptor (W0), transgenic cotton lines in which GhUBX is silenced or GhCDC48 is overexpressed exhibited significantly increased SCW thickness and fiber strength”
Claim · model-written summary
GhCDC48 silencing stunted cotton plant growth and impaired cell wall development.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GhCDC48 silencinge0
- Gene
- GhCDC48e0
- Outcome
- stunted plant growth and impaired cell wall developmente0
- Direction
- stuntede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhCDC48 silencing resulted in stunted plant growth and impaired cell wall development”
t0“cotton fiber”
Claim · model-written summary
GhCDC48 abundance correlated positively with ATPase activity in cotton fiber cells.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- fiber cellse0
- Gene
- GhCDC48e0
- Outcome
- ATPase activitye0
- Direction
- positively correlatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhCDC48 abundance positively correlates with ATPase activity in fiber cells”
t0“cotton fiber”
Claim · model-written summary
GhCDC48 abundance correlated positively with transcriptional activation of six named secondary cell wall biosynthesis genes.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhCDC48e0
- Outcome
- transcriptional activation of key SCW biosynthesis genese0
- Direction
- positively correlatese0
- Qualifier
- GhCesA7, GhCesA8, GhSusA, GhFSN1, GhKNL1, and GhNAC21e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhCDC48 abundance positively correlates with ATPase activity in fiber cells and with the transcriptional activation of key SCW biosynthesis genes including GhCesA7, GhCesA8, GhSusA, GhFSN1, GhKNL1, and GhNAC21”
t0“cotton fiber”
Claim · model-written summary
The authors propose modifying ubiquitin pathway genes to improve cotton fiber quality.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- improving fiber quality through targeted modification of genes in the ubiquitin pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“provide a genetic strategy for improving fiber quality through targeted modification of genes in the ubiquitin pathway”
t0“cotton fiber”
The same questions in other papers
loss of function · cytology · Gossypium hirsutum (cotton)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.