Abstract
Virus-induced gene silencing (VIGS) offers a powerful approach for functional analysis of individual genes by knocking down their expression. We have adopted this approach to dissect gene functions in cotton resistant to Verticillium wilt, one of the most devastating diseases worldwide. We showed here that highly efficient VIGS was obtained in a cotton breeding line (CA4002) with partial resistance to Verticillium wilt, and GhMKK2 and GhVe1 are required for its resistance to Verticillium wilt. Arabidopsis AtBAK1/SERK3, a central regulator in plant disease resistance, belongs to a subfamily of somatic embryogenesis receptor kinases (SERKs) with five members, AtSERK1 to AtSERK5. Two BAK1 orthologs and one SERK1 ortholog were identified in the cotton genome. Importantly, GhBAK1 is required for CA4002 resistance to Verticillium wilt. Surprisingly, silencing of GhBAK1 is sufficient to trigger cell death accompanied with production of reactive oxygen species in cotton. This result is distinct from Arabidopsis in which AtBAK1 and AtSERK4 play redundant functions in cell death control. Apparently, cotton has only evolved SERK1 and BAK1 whereas AtSERK4/5 are newly evolved genes in Arabidopsis. Our studies indicate the functional importance of BAK1 in Verticillium wilt resistance and suggest the dynamic evolution of SERK family members in different plant species.
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Claims 8
Claim · model-written summary
VIGS was highly efficient in cotton breeding line CA4002.
- Taxon
- cotton
Gossypium hirsutume0 - Material
- CA4002e0
- Outcome
- VIGSe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highly efficient VIGS was obtained in a cotton breeding line (CA4002) with partial resistance to Verticillium wilt”
Claim · model-written summary
GhMKK2 is required for resistance to Verticillium wilt in CA4002.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhMKK2e0
- Outcome
- resistance to Verticillium wilte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhMKK2 and GhVe1 are required for its resistance to Verticillium wilt”
t0“Cotton”
Claim · model-written summary
GhVe1 is required for resistance to Verticillium wilt in CA4002.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhVe1e0
- Outcome
- resistance to Verticillium wilte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhMKK2 and GhVe1 are required for its resistance to Verticillium wilt”
t0“Cotton”
Claim · model-written summary
Two BAK1 orthologs and one SERK1 ortholog were identified in the cotton genome.
- Taxon
- cottonGossypium hirsutume0
- Gene
- BAK1 orthologs and one SERK1 orthologe0
- Outcome
- identified in the cotton genomee0
- Value
- Two BAK1 orthologs and one SERK1 orthologe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two BAK1 orthologs and one SERK1 ortholog were identified in the cotton genome”
Claim · model-written summary
GhBAK1 is required for CA4002 resistance to Verticillium wilt.
- Taxon
- CottonGossypium hirsutumt0
- Material
- CA4002e0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhBAK1e0
- Outcome
- resistance to Verticillium wilte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBAK1 is required for CA4002 resistance to Verticillium wilt”
t0“Cotton”
Claim · model-written summary
Silencing GhBAK1 triggers cell death in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- silencing of GhBAK1e0
- Gene
- GhBAK1e0
- Outcome
- cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of GhBAK1 is sufficient to trigger cell death accompanied with production of reactive oxygen species in cotton”
Claim · model-written summary
Silencing GhBAK1 triggers reactive oxygen species production in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- silencing of GhBAK1e0
- Gene
- GhBAK1e0
- Outcome
- production of reactive oxygen speciese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of GhBAK1 is sufficient to trigger cell death accompanied with production of reactive oxygen species in cotton”
Claim · model-written summary
Cotton was inferred to have evolved SERK1 and BAK1 only, whereas AtSERK4/5 were newly evolved in Arabidopsis.
- Taxon
- cottonGossypium hirsutume0
- Gene
- SERK1 and BAK1e0
- Outcome
- only evolved SERK1 and BAK1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton has only evolved SERK1 and BAK1 whereas AtSERK4/5 are newly evolved genes in Arabidopsis”
The same questions in other papers
loss of function · cytology · Gossypium hirsutum (cotton)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.