Abstract
Abstract Background ATP-citrate lyase (ACL) plays a pivotal role in histone acetylation and aerobic glycolysis. In plant, ACL is a heteromeric enzyme composed of ACLA (45 kD) and ACLB (65 kD). So far, the function of ACL genes in cotton still remains unknown. Results Here, we identified three ACLA homologous sequences and two ACLB homologous in each genome/sub-genome of cotton species. Silencing ACLB in cotton led to cell death at newly-grown leaves and stem apexes. Simultaneously, in ACLB-silenced plants, transcription factors related to senescence including SGR , WRKY23 and Osl57 were observed to be activated. Further investigation showed that excessive H 2 O 2 was accumulated, salicylic acid-dependent defense response and pathogenesis-related gene expressions were evidently enhanced in ACLB-silenced plants, implying that knockdown of ACLB genes leads to hypersensitive response-like cell death in cotton seedlings. However, as noted, serious cell death happened in newly-grown leaves and stem apexes in ACLB-silenced plants, which led to the failure of subsequent fungal pathogenicity assays. To confirm the role of ACLB gene in regulating plant immune response, the dicotyledonous model plant Arabidopsis was selected for functional verification of ACLB gene. Our results indicate the resistance to Verticillium dahliae infection in the Arabidopsis mutant aclb-2 were enhanced without causing strong cell death. Ectopic expression of GausACLB-2 in Arabidopsis weakened its resistance to V. dahliae either in Col-0 or in aclb-2 background, in which the expression level of ACLB is negatively correlated with the resistance to V. dahliae . Conclusions These results indicate that ACLB has a new function in negatively affecting the induction of plant defense response and cell death in cotton, which provides theoretical guidance for developing cotton varieties with resistance against Verticillium wilt.
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Claims 9
Claim · model-written summary
Three ACLA homologous sequences and two ACLB homologs were identified in each cotton genome or subgenome.
- Taxon
- cotton species
Gossypium hirsutume0 - Gene
- ACLAe0
- Outcome
- homologous sequencese0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified three ACLA homologous sequences and two ACLB homologous in each genome/sub-genome of cotton species”
Claim · model-written summary
Cotton ACLB silencing caused cell death in new leaves and stem apices.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- newly-grown leaves and stem apexese0
- Treatment
- Silencing ACLBe0
- Gene
- ACLBe0
- Outcome
- cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing ACLB in cotton led to cell death at newly-grown leaves and stem apexes.”
Claim · model-written summary
ACLB silencing activated senescence-related transcription factors SGR, WRKY23 and Osl57.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- ACLB-silencede0
- Gene
- ACLBe0
- Outcome
- transcription factors related to senescence including SGR , WRKY23 and Osl57e0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in ACLB-silenced plants, transcription factors related to senescence including SGR , WRKY23 and Osl57 were observed to be activated”
e1“in cotton led to cell death”
Claim · model-written summary
Hydrogen peroxide accumulated excessively in ACLB-silenced cotton plants.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- ACLB-silenced plantse2
- Outcome
- H 2 O 2e0
- Direction
- excessivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excessive H 2 O 2 was accumulated”
e1“in cotton led to cell death”
e2“were evidently enhanced in ACLB-silenced plants”
Claim · model-written summary
Salicylic acid-dependent defense response and pathogenesis-related gene expression increased in ACLB-silenced plants.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- ACLB-silenced plantse0
- Outcome
- salicylic acid-dependent defense response and pathogenesis-related gene expressionse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salicylic acid-dependent defense response and pathogenesis-related gene expressions were evidently enhanced in ACLB-silenced plants”
e1“in cotton led to cell death”
Claim · model-written summary
Arabidopsis aclb-2 mutants showed enhanced resistance to Verticillium dahliae infection without strong cell death.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- aclb-2e0
- Interactor
- Verticillium dahliae
Verticillium dahliaee0pathogen - Outcome
- resistance to Verticillium dahliae infectione0
- Direction
- enhancede0
- Qualifier
- without causing strong cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance to Verticillium dahliae infection in the Arabidopsis mutant aclb-2 were enhanced without causing strong cell death”
Claim · model-written summary
Ectopic GausACLB-2 expression weakened Arabidopsis resistance to V. dahliae in Col-0.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Col-0e0
- Treatment
- Ectopic expression of GausACLB-2e0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GausACLB-2e0
- Outcome
- resistance to V. dahliaee0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of GausACLB-2 in Arabidopsis weakened its resistance to V. dahliae either in Col-0 or in aclb-2 background”
Claim · model-written summary
Ectopic GausACLB-2 expression weakened Arabidopsis resistance to V. dahliae in the aclb-2 background.
- Taxon
- ArabidopsisArabidopsise0
- Material
- aclb-2e0
- Treatment
- Ectopic expression of GausACLB-2e0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GausACLB-2e0
- Outcome
- resistance to V. dahliaee0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of GausACLB-2 in Arabidopsis weakened its resistance to V. dahliae either in Col-0 or in aclb-2 background”
Claim · model-written summary
ACLB expression was negatively correlated with resistance to V. dahliae.
- Taxon
- ArabidopsisArabidopsise1
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- ACLBe0
- Outcome
- resistance to V. dahliaee0
- Direction
- negatively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression level of ACLB is negatively correlated with the resistance to V. dahliae”
e1“in Arabidopsis weakened its resistance”
The same questions in other papers
loss of function · cytology · Gossypium hirsutum (cotton)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
4 papers more in this group
See all 8 papers →overexpression · Verticillium dahliae · ArabidopsisResults agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.