Abstract
Recent studies have observed rhizosphere microorganisms and metabolic pathways of tobacco plants affect the host’s resistance against Ralstonia solanacearum . However, the interaction between root associated microbiota and plant in confronting the bacterial wilt disease is largely unknown. Here, integrative analyses of rhizosphere microbiota and transcriptome of tobacco plant were conducted after inoculating with R. solanacearum or sterile water (RS and CK, respectively). Comparative analysis demonstrated that there was significant difference between rhizosphere bacterial communities of RS and CK. The relative abundances of some potential beneficial bacteria, e.g., Caballeronia , Sphingomonas , and Devosia , were significantly higher both in root and rhizosphere soil samples of CK, compared to RS. While the relative abundances of Flavobacterium , and Caulobacter in root samples and Stenotrophomonas in rhizosphere soil samples showed opposite trend. Furthermore, weighted gene co-expression network analysis (WGCNA) was used to identify the key metabolic pathway response to R. solanacearum invasion. The results revealed that phenylpropanoid metabolic pathway in tobacco interacted with rhizosphere key species, and the expressions of structural genes related to this pathway were tested by qRT-PCR. Overall, the microbiome and transcriptome data described in our study indicate that both specific root-associated bacterial consortium and phenylpropanoid metabolic pathway play an important role in resistance against R. solanacearum for tobacco plants. • •The shift of soil nutrient contents is related to specific root-associated microbiota. • • Ralstonia solanacearum invasion caused rhizosphere microbiome and leaf transcriptome changes in tobacco. • •The mechanisms of integrative response between specific root-associated microbiota and phenylpropanoid metabolic pathway was proposed.
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Claims 11
Claim · model-written summary
Ralstonia inoculation significantly changed tobacco rhizosphere bacterial communities.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- RSe0
- Interactor
- Ralstonia solanacearum
Ralstonia solanacearumt0pathogen - Outcome
- rhizosphere bacterial communitiese0
- Comparator
- CKe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was significant difference between rhizosphere bacterial communities of RS and CK”
t0“Ralstonia solanacearum of tobacco”
Claim · model-written summary
Caballeronia abundance in tobacco roots and rhizosphere soil was higher in CK than RS.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- CaballeroniaCaballeroniae0symbiont
- Outcome
- relative abundances of some potential beneficial bacteriae0
- Direction
- highere0
- Qualifier
- both in root and rhizosphere soil samples of CKe0
- Comparator
- RSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative abundances of some potential beneficial bacteria, e.g., Caballeronia , Sphingomonas , and Devosia , were significantly higher both in root and rhizosphere soil samples of CK, compared to RS”
t0“tobacco”
Claim · model-written summary
Sphingomonas abundance in tobacco roots and rhizosphere soil was higher in CK than RS.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- Sphingomonas
Sphingomonase0symbiont - Outcome
- relative abundances of some potential beneficial bacteriae0
- Direction
- highere0
- Comparator
- RSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative abundances of some potential beneficial bacteria, e.g., Caballeronia , Sphingomonas , and Devosia , were significantly higher both in root and rhizosphere soil samples of CK, compared to RS”
t0“tobacco”
Claim · model-written summary
Devosia abundance in tobacco roots and rhizosphere soil was higher in CK than RS.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- relative abundances of some potential beneficial bacteriae0
- Direction
- highere0
- Comparator
- RSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative abundances of some potential beneficial bacteria, e.g., Caballeronia , Sphingomonas , and Devosia , were significantly higher both in root and rhizosphere soil samples of CK, compared to RS”
t0“tobacco”
Claim · model-written summary
Flavobacterium abundance in roots had the opposite trend from beneficial bacteria.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- root samplese0
- Interactor
- FlavobacteriumFlavobacteriume0other
- Outcome
- relative abundances of Flavobacteriume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative abundances of Flavobacterium , and Caulobacter in root samples and Stenotrophomonas in rhizosphere soil samples showed opposite trend”
t0“tobacco”
Claim · model-written summary
Caulobacter abundance in roots had the opposite trend from beneficial bacteria.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- root samplese0
- Interactor
- Caulobacter
Caulobactere0other - Outcome
- relative abundances of Flavobacterium , and Caulobactere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative abundances of Flavobacterium , and Caulobacter in root samples and Stenotrophomonas in rhizosphere soil samples showed opposite trend”
t0“tobacco”
Claim · model-written summary
Stenotrophomonas abundance in rhizosphere soil had the opposite trend from beneficial bacteria.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- Stenotrophomonas
Stenotrophomonase0other - Outcome
- Stenotrophomonas in rhizosphere soil samplese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative abundances of Flavobacterium , and Caulobacter in root samples and Stenotrophomonas in rhizosphere soil samples showed opposite trend”
t0“tobacco”
Claim · model-written summary
The tobacco phenylpropanoid pathway interacted with key rhizosphere species.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- rhizosphere key speciese0symbiont
- Outcome
- phenylpropanoid metabolic pathwaye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenylpropanoid metabolic pathway in tobacco interacted with rhizosphere key species”
Claim · model-written summary
Soil nutrient changes were related to specific root microbiota.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- root-associated microbiotae0symbiont
- Outcome
- soil nutrient contentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shift of soil nutrient contents is related to specific root-associated microbiota”
t0“tobacco”
Claim · model-written summary
Ralstonia invasion changed the tobacco leaf transcriptome.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Ralstonia solanacearum invasione0
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Outcome
- leaf transcriptome changese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum invasion caused rhizosphere microbiome and leaf transcriptome changes in tobacco”
Claim · model-written summary
The authors infer that root bacteria and phenylpropanoid metabolism contribute to tobacco resistance.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Outcome
- resistance against R. solanacearume0
- Qualifier
- specific root-associated bacterial consortium and phenylpropanoid metabolic pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both specific root-associated bacterial consortium and phenylpropanoid metabolic pathway play an important role in resistance against R. solanacearum for tobacco plants”