Abstract
Silicates (Si) improve plant growth; however, how different types of silicate sources influence plant growth and rhizosphere microbiome remains underexplored. We compare two Si types (pure and bioavailable silicic acid (Si) and mineral magnesium silicate (MgSi)) applied to the soybean (Glycine max L.) rhizosphere to determine whether two silicate types (Si-types) differently impact plant growth, defense responses, and microbiome diversity and function during heat stress. Under heat stress, Si-type treatments improved biomass (86 % with Si and 82 % with MgSi), reduced H₂O₂ (26 % phyllosphere; 33 % rhizosphere), and enhanced β-glucosidase activity (2.6-fold rhizosphere) compared to heat-only treatment and increased Proteobacteria relative abundance from ∼65 % (heat-only) to ∼74 % in Si-type-treated rhizospheric soil. Si-types showed downregulation of heat shock transcription factors, suggesting regulatory defense effects during heat stress. Metagenome-assembled genomes (MAGs) analysis revealed significant bacterial shifts across the Si-treatments, with Proteobacteria and Bacteroidetes being the dominant phyla in the rhizospheric soil. Under heat stress, the core microbiome comprised 14 rhizosphere genera (including Pelomonas, Achromobacter, Paracoccus, Nocardioides), whereas Pelomonas was the sole core root genus, and Pelomonas puraquae core species in both compartments. MAGs analysis revealed Si-based shifts in microbial metabolic pathways and enrichment of auxin biosynthesis in Si-treated roots during heat stress. Because MgSi supplies both Mg and Si, effects observed with MgSi are interpreted as combined Si + Mg effects. In conclusion, both Si-types caused shifts in microbiome diversity and function, and impacted plant growth and defense responses under heat stress, providing a foundation for improving thermotolerance in plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Silicic acid raised biomass by 86% under heat stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under heat stress, Si-type treatments improved biomass (86 % with Si and 82 % with MgSi)”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Magnesium silicate raised biomass by 82% under heat stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under heat stress, Si-type treatments improved biomass (86 % with Si and 82 % with MgSi)”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicate treatments reduced phyllosphere H₂O₂ by 26%.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced H₂O₂ (26 % phyllosphere; 33 % rhizosphere)”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicate treatments reduced rhizosphere H₂O₂ by 33%.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced H₂O₂ (26 % phyllosphere; 33 % rhizosphere)”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicates enhanced rhizosphere β-glucosidase activity 2.6 fold.
- Taxon
- soybean
Glycine maxt0 - Outcome
- β-glucosidase activitye0
- Direction
- enhancede0
- Value
- 2.6-folde0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced β-glucosidase activity (2.6-fold rhizosphere)”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicates raised rhizospheric Proteobacteria abundance from about 65% to about 74%.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Si-type-treatede0
- Interactor
- ProteobacteriaPseudomonadotae0other
- Outcome
- Proteobacteria relative abundancee0
- Direction
- increasede0
- Value
- ∼74 %e0
- Qualifier
- ∼65 %e0
- Comparator
- heat-onlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased Proteobacteria relative abundance from ∼65 % (heat-only) to ∼74 % in Si-type-treated rhizospheric soil”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicate types downregulated heat shock transcription factors.
- Taxon
- soybeanGlycine maxt0
- Gene
- heat shock transcription factorse0
- Outcome
- heat shock transcription factorse0
- Direction
- downregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Si-types showed downregulation of heat shock transcription factors”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Si treatments shifted rhizosphere bacterial composition significantly.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Si-treatmentse0
- Outcome
- bacterial shiftse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant bacterial shifts across the Si-treatments”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Proteobacteria and Bacteroidetes dominated rhizosphere soil.
- Taxon
- soybeanGlycine maxt0
- Interactor
- ProteobacteriaPseudomonadotae0other
- Second interactor
- BacteroidetesBacteroidotae0other
- Outcome
- dominant phylae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proteobacteria and Bacteroidetes being the dominant phyla in the rhizospheric soil”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
The heat stress rhizosphere core microbiome had 14 genera.
- Taxon
- soybeanGlycine maxt0
- Outcome
- rhizosphere generae0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under heat stress, the core microbiome comprised 14 rhizosphere genera”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Pelomonas was the only core root genus.
- Taxon
- soybeanGlycine maxt0
- Interactor
- PelomonasRoseatelese0other
- Outcome
- sole core root genuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pelomonas was the sole core root genus”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Pelomonas puraquae was core in both compartments.
- Taxon
- soybeanGlycine maxt0
- Interactor
- Pelomonas puraquaeRoseateles puraquaee0other
- Outcome
- core species in both compartmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pelomonas puraquae core species in both compartments”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicate treatments shifted microbial metabolic pathways.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Si-basede0
- Outcome
- microbial metabolic pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Si-based shifts in microbial metabolic pathways”
t0“the soybean (Glycine max L.) rhizosphere”
Claim · model-written summary
Silicate treated roots were enriched for auxin biosynthesis under heat stress.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- rootse0
- Outcome
- auxin biosynthesise0
- Direction
- enrichmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enrichment of auxin biosynthesis in Si-treated roots during heat stress”
t0“the soybean (Glycine max L.) rhizosphere”
The same questions in other papers
silicon atom · growth · Glycine max (soybean)Results differ
23 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
24 papers more in this group
See all 28 papers →magnesium atom · growth · Glycine max (soybean)Positive and negative results
20 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- negative
- negative
25 papers more in this group
See all 29 papers →silicate(4-) · growth · Glycine max (soybean)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.