Abstract
Plant hormone-loaded nanoparticles (NPs) represent a novel class of materials with significant potential in plant cell culture, owing to their unique physico-chemical properties. The utilization of these hormone-loaded NPs as elicitors could enhance the production of bioactive compounds and boost antioxidant enzymatic activity in plant cell suspension cultures. Therefore, this study aimed to synthesize jasmonic acid (JA) loaded Fe 3 O 4 NPs and evaluate their effects on the cell suspension culture of Carthamus tinctorius (safflower). The synthesized material was applied at various concentrations (10, 20, 40 and 80 mg L -1 ) to assess its impact on cell growth, physio-biochemical, antioxidative activities and specialized metabolites (SMs) of C. tinctorius . The results demonstrated that the addition of JA-loaded NPs significantly enhanced the total chlorophyll (70.37%), soluble protein (154.45%) and total phenolic contents (110.64%) of safflower compared to the control. A linear decrease in all reactive oxygen species (ROS) attributes, such as H 2 O 2 (4.65%) and O 2 - (22.81%), was observed as the NPs concentration in the culture media was increased to the T2 group (20 mg L -1 ). Maximum chlorogenic acid (CGAs) accumulation (43.76 mg g -1 ) was noted on 72 hours after elicitation, representing a 2.26-fold increase over the control group. Furthermore, amino acid profiling revealed substantial variations in the composition of all detected amino acids following treatment with JA-loaded Fe 3 O 4 NPs. In summary, this strategy demonstrates potential for optimizing the production of antioxidant and bioactive metabolites, thereby offering a viable solution for the industrial scale production of high-quality safflower extracts.
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Claims 7
Claim · model-written summary
JA-loaded nanoparticles increased total chlorophyll by 70.37% versus control.
- Taxon
- safflower
Carthamus tinctoriuse0 - Treatment
- JA-loaded NPse0
- Outcome
- total chlorophylle0
- Direction
- enhancede0
- Value
- 70.37%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA-loaded NPs significantly enhanced the total chlorophyll (70.37%), soluble protein (154.45%) and total phenolic contents (110.64%) of safflower compared to the control”
Claim · model-written summary
JA-loaded nanoparticles increased soluble protein by 154.45% versus control.
- Taxon
- safflowerCarthamus tinctoriuse0
- Treatment
- JA-loaded NPse0
- Outcome
- soluble proteine0
- Direction
- enhancede0
- Value
- 154.45%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA-loaded NPs significantly enhanced the total chlorophyll (70.37%), soluble protein (154.45%) and total phenolic contents (110.64%) of safflower compared to the control”
Claim · model-written summary
JA-loaded nanoparticles increased total phenolic contents by 110.64% versus control.
- Taxon
- safflowerCarthamus tinctoriuse0
- Treatment
- JA-loaded NPse0
- Outcome
- total phenolic contentse0
- Direction
- enhancede0
- Value
- 110.64%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA-loaded NPs significantly enhanced the total chlorophyll (70.37%), soluble protein (154.45%) and total phenolic contents (110.64%) of safflower compared to the control”
Claim · model-written summary
Increasing nanoparticle concentration to 20 mg L -1 decreased H 2 O 2 by 4.65%.
- Taxon
- Carthamus tinctoriusCarthamus tinctoriuse1
- Treatment
- NPs concentration in the culture media was increased to the T2 group (20 mg L -1 )e0
- Outcome
- H 2 O 2e0
- Direction
- decreasee0
- Value
- 4.65%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A linear decrease in all reactive oxygen species (ROS) attributes, such as H 2 O 2 (4.65%) and O 2 - (22.81%), was observed as the NPs concentration in the culture media was increased to the T2 group (20 mg L -1 )”
e1“cell suspension culture of Carthamus tinctorius (safflower)”
Claim · model-written summary
Increasing nanoparticle concentration to 20 mg L -1 decreased O 2 - by 22.81%.
- Taxon
- Carthamus tinctoriusCarthamus tinctoriuse1
- Treatment
- NPs concentration in the culture media was increased to the T2 group (20 mg L -1 )e0
- Outcome
- O 2 -e0
- Direction
- decreasee0
- Value
- 22.81%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A linear decrease in all reactive oxygen species (ROS) attributes, such as H 2 O 2 (4.65%) and O 2 - (22.81%), was observed as the NPs concentration in the culture media was increased to the T2 group (20 mg L -1 )”
e1“cell suspension culture of Carthamus tinctorius (safflower)”
Claim · model-written summary
Chlorogenic acid accumulation reached 43.76 mg g -1 at 72 hours, 2.26-fold over control.
- Taxon
- Carthamus tinctoriusCarthamus tinctoriuse1
- Treatment
- elicitatione0
- Outcome
- chlorogenic acid (CGAs) accumulatione0
- Direction
- increasee0
- Value
- 43.76 mg g -1e0
- Qualifier
- 2.26-folde0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum chlorogenic acid (CGAs) accumulation (43.76 mg g -1 ) was noted on 72 hours after elicitation, representing a 2.26-fold increase over the control group”
e1“cell suspension culture of Carthamus tinctorius (safflower)”
Claim · model-written summary
JA-loaded nanoparticles caused substantial variation in amino acid composition.
- Taxon
- Carthamus tinctoriusCarthamus tinctoriuse1
- Treatment
- JA-loaded Fe 3 O 4 NPse0
- Outcome
- composition of all detected amino acidse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amino acid profiling revealed substantial variations in the composition of all detected amino acids following treatment with JA-loaded Fe 3 O 4 NPs”
e1“cell suspension culture of Carthamus tinctorius (safflower)”
The same questions in other papers
nanoparticle · physiology: oxidative · Carthamus tinctorius (safflower)Positive and negative results
1 in the opposite direction
- negative
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1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
nanoparticle · physiology: osmolytes · Carthamus tinctorius (safflower)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.