Abstract
MicroRNAs (miRNAs) represent the most abundant class of small RNAs in plants, in which the miR159 family demonstrates a high degree of conservation across various plant species and plays a crucial role in regulating anther, silique, and seed development by targeting GAMYB-like. The members of the Fan-miR159 family in cultivated strawberry target not only GAMYB-like genes but also Auxin Response Factor (ARF) family members, which are annotated as “ARF23-like” in the genome. In this study, we firstly analyzed the structural features, evolutionary conservation, and expression patterns of the miR159 family in Fragaria × ananassa. Then, we reannotated the “ARF23-like” genes in cultivated strawberry and found that they actually belong to the “ARF2b” subfamily. Finally, we utilized RT-qPCR and co-transformation experiments to analyze and validate our observation. The results showed the suppression of FaARF2b-2 expression by fan-miR159n and fan-miR159r in both tobacco and strawberry. Moreover, two transcripts of the FaARF2b-2 gene were detected in cultivated strawberry, FaARF2b-2R and FaARF2b-2M. The expression levels of both FaARF2b-2M and FaARF2b-2R increased following the abscisic acid (ABA) treatment, indicating that they may be positively regulated by ABA. In addition, FaARF2b-2M exhibits significantly higher expression levels in the anther compared to FaARF2b-2R, which potentially plays an important role in the male reproductive development. Our findings enhance the understanding of the miR159 family in cultivated strawberry and expand the knowledge regarding its novel targets.
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Claims 7
Claim · model-written summary
The strawberry ARF23-like genes belong to the ARF2b subfamily.
- Taxon
- cultivated strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Gene
- “ARF23-like” genese0
- Outcome
- belong to the “ARF2b” subfamilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“reannotated the “ARF23-like” genes in cultivated strawberry and found that they actually belong to the “ARF2b” subfamily”
Claim · model-written summary
fan-miR159n suppressed FaARF2b-2 expression in tobacco and strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaARF2b-2e0
- Outcome
- suppression of FaARF2b-2 expressione0
- Qualifier
- fan-miR159ne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of FaARF2b-2 expression by fan-miR159n and fan-miR159r in both tobacco and strawberry”
Claim · model-written summary
fan-miR159r suppressed FaARF2b-2 expression in tobacco and strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaARF2b-2e0
- Outcome
- suppression of FaARF2b-2 expressione0
- Qualifier
- fan-miR159re0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of FaARF2b-2 expression by fan-miR159n and fan-miR159r in both tobacco and strawberry”
Claim · model-written summary
Two FaARF2b-2 transcripts, FaARF2b-2R and FaARF2b-2M, were detected in cultivated strawberry.
- Taxon
- cultivated strawberryFragaria ×ananassae0
- Gene
- FaARF2b-2 genee0
- Outcome
- two transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two transcripts of the FaARF2b-2 gene were detected in cultivated strawberry, FaARF2b-2R and FaARF2b-2M”
Claim · model-written summary
FaARF2b-2M expression increased following ABA treatment.
- Treatment
- abscisic acid (ABA) treatmente0
- Gene
- FaARF2b-2Me0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“expression levels of both FaARF2b-2M and FaARF2b-2R increased following the abscisic acid (ABA) treatment”
Claim · model-written summary
FaARF2b-2R expression increased following ABA treatment.
- Treatment
- abscisic acid (ABA) treatmente0
- Gene
- FaARF2b-2Re0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“expression levels of both FaARF2b-2M and FaARF2b-2R increased following the abscisic acid (ABA) treatment”
Claim · model-written summary
FaARF2b-2M expression in anthers was significantly higher than FaARF2b-2R expression.
- Organ / stage
- anthere0
- Gene
- FaARF2b-2Me0
- Outcome
- expression levelse0
- Direction
- highere0
- Comparator
- FaARF2b-2Re0
Not reportedTaxon
Verbatim
e0“FaARF2b-2M exhibits significantly higher expression levels in the anther compared to FaARF2b-2R”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.