Abstract
The receptacle of the strawberry (Fragaria × ananassa) fruit accounts for the main properties of the ripe fruit for human consumption. As it ripens, it undergoes changes similar to other fruits in sugar : acid ratio, volatile production and cell wall softening. However, the main regulators of this process have not yet been reported. The white stage marks the initiation of the ripening process, and we had previously reported a peak of expression for a FaGAMYB gene. Transient silencing of FaGAMYB using RNAi and further determination of changes in global gene expression by RNAseq, and composition of primary and secondary metabolites have been used to investigate the role played by this gene during the development of the receptacle. Down-regulation of FaGAMYB caused an arrest in the ripening of the receptacle and inhibited colour formation. Consistent with this, several transcription factors associated with the regulation of flavonoid biosynthetic pathway showed altered expression. FaGAMYB silencing also caused a reduction of ABA biosynthesis and sucrose content. Interestingly, exogenous ABA application to the RNAI-transformed receptacle reversed most defects caused by FaGAMYB down-regulation. The study assigns a key regulatory role to FaGAMYB in the initiation of strawberry receptacle ripening and acting upstream of the known regulator ABA.
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Claims 7
Claim · model-written summary
FaGAMYB down-regulation arrested strawberry receptacle ripening.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae1 - Treatment
- Down-regulation of FaGAMYBe0
- Gene
- FaGAMYBe0
- Outcome
- ripening of the receptaclee0
- Direction
- arreste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Down-regulation of FaGAMYB caused an arrest in the ripening of the receptacle”
e1“the strawberry (Fragaria × ananassa) fruit”
Claim · model-written summary
FaGAMYB down-regulation inhibited color formation in strawberry receptacles.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- Down-regulation of FaGAMYBe0
- Gene
- FaGAMYBe0
- Outcome
- colour formatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of FaGAMYB caused an arrest in the ripening of the receptacle and inhibited colour formation.”
e1“the strawberry (Fragaria × ananassa) fruit”
Claim · model-written summary
Flavonoid-pathway transcription factors showed altered expression after FaGAMYB silencing.
- Taxon
- strawberryFragaria ×ananassae2
- Treatment
- FaGAMYB silencinge1
- Gene
- transcription factorse0
- Outcome
- altered expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several transcription factors associated with the regulation of flavonoid biosynthetic pathway showed altered expression.”
e1“FaGAMYB silencing”
e2“the strawberry (Fragaria × ananassa) fruit”
Claim · model-written summary
FaGAMYB silencing reduced ABA biosynthesis in strawberry receptacles.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- FaGAMYB silencinge0
- Gene
- FaGAMYBe0
- Outcome
- ABA biosynthesise0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaGAMYB silencing also caused a reduction of ABA biosynthesis and sucrose content.”
e1“the strawberry (Fragaria × ananassa) fruit”
Claim · model-written summary
FaGAMYB silencing reduced sucrose content in strawberry receptacles.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- FaGAMYB silencinge0
- Gene
- FaGAMYBe0
- Outcome
- sucrose contente0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaGAMYB silencing also caused a reduction of ABA biosynthesis and sucrose content.”
e1“the strawberry (Fragaria × ananassa) fruit”
Claim · model-written summary
Exogenous ABA reversed most defects caused by FaGAMYB down-regulation in strawberry receptacles.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- exogenous ABA applicatione0
- Gene
- FaGAMYBe0
- Outcome
- defects caused by FaGAMYB down-regulatione0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous ABA application to the RNAI-transformed receptacle reversed most defects caused by FaGAMYB down-regulation.”
e1“the strawberry (Fragaria × ananassa) fruit”
Claim · model-written summary
The authors place FaGAMYB upstream of ABA in initiating strawberry receptacle ripening.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaGAMYBe0
- Compound
- ABAe0
- Outcome
- initiation of strawberry receptacle ripeninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The study assigns a key regulatory role to FaGAMYB in the initiation of strawberry receptacle ripening and acting upstream of the known regulator ABA.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.