Abstract
BACKGROUND: ABA-, stress- and ripening-induced (ASR) proteins have been reported to act as a downstream component involved in ABA signal transduction. Although much attention has been paid to the roles of ASR in plant development and stress responses, the mechanisms by which ABA regulate fruit ripening at the molecular level are not fully understood. In the present work, a strawberry ASR gene was isolated and characterized (FaASR), and a polyclonal antibody against FaASR protein was prepared. Furthermore, the effects of ABA, applied to two different developmental stages of strawberry, on fruit ripening and the expression of FaASR at transcriptional and translational levels were investigated. METHODOLOGY/PRINCIPAL FINDINGS: FaASR, localized in the cytoplasm and nucleus, contained 193 amino acids and shared common features with other plant ASRs. It also functioned as a transcriptional activator in yeast with trans-activation activity in the N-terminus. During strawberry fruit development, endogenous ABA content, levels of FaASR mRNA and protein increased significantly at the initiation of ripening at a white (W) fruit developmental stage. More importantly, application of exogenous ABA to large green (LG) fruit and W fruit markedly increased endogenous ABA content, accelerated fruit ripening, and greatly enhanced the expression of FaASR transcripts and the accumulation of FaASR protein simultaneously. CONCLUSIONS: These results indicate that FaASR may be involved in strawberry fruit ripening. The observed increase in endogenous ABA content, and enhanced FaASR expression at transcriptional and translational levels in response to ABA treatment might partially contribute to the acceleration of strawberry fruit ripening.
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Claims 9
Claim · model-written summary
FaASR localized in the cytoplasm and nucleus.
- Taxon
- Strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Gene
- FaASRe0
- Outcome
- localized in the cytoplasm and nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“FaASR, localized in the cytoplasm and nucleus, contained 193 amino acids and shared common features with other plant ASRs”
t0“Strawberry FaASR Gene”
Claim · model-written summary
FaASR contained 193 amino acids.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FaASRe0
- Outcome
- amino acidse0
- Value
- 193 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaASR, localized in the cytoplasm and nucleus, contained 193 amino acids”
t0“Strawberry FaASR Gene”
Claim · model-written summary
FaASR shared common features with other plant ASRs.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FaASRt1
- Outcome
- shared common features with other plant ASRse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shared common features with other plant ASRs”
t0“Strawberry FaASR Gene”
t1“FaASR Gene”
Claim · model-written summary
FaASR functioned as a transcriptional activator in yeast through its N terminus.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FaASRt1
- Outcome
- transcriptional activator in yeaste0
- Qualifier
- N-terminuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also functioned as a transcriptional activator in yeast with trans-activation activity in the N-terminus”
t0“Strawberry FaASR Gene”
t1“FaASR Gene”
Claim · model-written summary
At the white stage of ripening, endogenous ABA content and FaASR mRNA and protein levels increased significantly.
- Taxon
- StrawberryFragaria ×ananassat0
- Organ / stage
- white (W) fruit developmental stagee0
- Gene
- FaASRe0
- Outcome
- endogenous ABA content, levels of FaASR mRNA and proteine0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous ABA content, levels of FaASR mRNA and protein increased significantly at the initiation of ripening at a white (W) fruit developmental stage”
t0“Strawberry FaASR Gene”
Claim · model-written summary
ABA application increased endogenous ABA content in large green fruit.
- Taxon
- StrawberryFragaria ×ananassat0
- Organ / stage
- large green (LG) fruite0
- Treatment
- exogenous ABAe0
- Outcome
- endogenous ABA contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of exogenous ABA to large green (LG) fruit and W fruit markedly increased endogenous ABA content, accelerated fruit ripening, and greatly enhanced the expression of FaASR transcripts and the accumulation of FaASR protein simultaneously”
t0“Strawberry FaASR Gene”
Claim · model-written summary
ABA application accelerated ripening in white-stage fruit.
- Taxon
- StrawberryFragaria ×ananassat0
- Organ / stage
- W fruite0
- Treatment
- exogenous ABAe0
- Outcome
- fruit ripeninge0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of exogenous ABA to large green (LG) fruit and W fruit markedly increased endogenous ABA content, accelerated fruit ripening, and greatly enhanced the expression of FaASR transcripts and the accumulation of FaASR protein simultaneously”
t0“Strawberry FaASR Gene”
Claim · model-written summary
ABA application enhanced FaASR transcript expression and protein accumulation in strawberry fruit.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- exogenous ABAe0
- Gene
- FaASRe0
- Outcome
- expression of FaASR transcripts and the accumulation of FaASR proteine0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of exogenous ABA to large green (LG) fruit and W fruit markedly increased endogenous ABA content, accelerated fruit ripening, and greatly enhanced the expression of FaASR transcripts and the accumulation of FaASR protein simultaneously”
t0“Strawberry FaASR Gene”
Claim · model-written summary
FaASR may be involved in strawberry fruit ripening.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaASRe0
- Outcome
- fruit ripeninge0
- Qualifier
- may be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaASR may be involved in strawberry fruit ripening”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.