Abstract
ABSTRACT Bacterial panicle blight (BPB), caused by Burkholderia glumae , is an emerging disease in rice that threatens global food security in the absence of effective strategies to control it. The environmental bacterium Pseudomonas protegens PBL3 produces and secretes a collection of molecules (secretome) with demonstrated antimicrobial activity in vitro against B. glumae , suggesting that this P. protegens PBL3 secretome can be exploited as a source of an environmentally friendly management strategy against BPB. However, the effectiveness of the P. protegens PBL3 secretome under greenhouse and field conditions has not been investigated, as they require large volumes of the active product. In this work, we optimised fermentation conditions to successfully scale up the production of P. protegens PBL3 secretome from 20 mL to 6 L while retaining its antimicrobial activity. Lyophilisation of the P. protegens PBL3 secretome obtained at large scale facilitated testing of several concentrations to identify the minimal inhibitory concentration in vitro and in planta. We further demonstrated that spraying the P. protegens PBL3 secretome at 0.1 g/mL in rice panicles prior to B. glumae inoculation significantly reduced B. glumae populations and BPB symptoms. These results will pave the way towards advancing the use of the P. protegens PBL3 secretome to alleviate the impact of BPB.
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Claims 2
Claim · model-written summary
The P. protegens PBL3 secretome was scaled up from 20 mL to 6 L while retaining antimicrobial activity.
- Taxon
- Rice
Oryza sativat0 - Outcome
- antimicrobial activitye0
- Direction
- retaininge0
- Value
- 6 Le0
- Comparator
- 20 mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacterial Panicle Blight in Rice”
e0“scale up the production of P. protegens PBL3 secretome from 20 mL to 6 L while retaining its antimicrobial activity”
Claim · model-written summary
Spraying the P. protegens PBL3 secretome on rice panicles before inoculation reduced B. glumae populations and BPB symptoms.
- Taxon
- RiceOryza sativat0
- Treatment
- spraying the P. protegens PBL3 secretome at 0.1 g/mLe1
- Interactor
- B. glumae
Burkholderia glumaee1pathogen - Second interactor
- P. protegens PBL3Pseudomonas protegense1biocontrol_agent
- Outcome
- B. glumae populations and BPB symptomse1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacterial Panicle Blight in Rice”
e1“spraying the P. protegens PBL3 secretome at 0.1 g/mL in rice panicles prior to B. glumae inoculation significantly reduced B. glumae populations and BPB symptoms”
The same questions in other papers
application · Burkholderia glumae · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- positive
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.