Abstract
Methane released from rice paddy soils is a major contributor to anthropogenic greenhouse gas accumulation, which accelerates global warming. In paddy soils, anaerobic methanogenic archaea produce methane that is simultaneously oxidized by methanotrophic bacteria. The unoxidized methane is released into the atmosphere through aerenchyma channels of rice. Since controlling the abundance and activity of methanotrophs in soils remains technically challenging, we aimed to identify root-colonizing heterotrophic bacteria that increase the growth of methanotrophs to mitigate methane emission. The screening of 960 aerobic and 960 anaerobic heterotrophic bacteria depending on colony morphology identified Peribacillus frigoritolerans AE95 as a rhizobacterium promoting the growth of methanotroph in vitro and reducing methane emission by up to 66.8 % when applied to the rice root system in vivo . Genome-scale metabolic modelling predicted that l -alanine as a key growth-supporting metabolite secreted by P. frigoritolerans AE95, which was experimentally validated to enhance Methylococcus geothermalis and Methylocystis parvus proliferation. In addition to methane mitigation, P. frigoritolerans and l -alanine supplementation elicited rice innate immunity against a bacterial pathogen Xanthomonas oryzae pv. oryzae . Our findings indicate that the rhizosphere heterotroph P. frigoritolerans AE95 possesses dual functionality, contributing to both methane mitigation and the enhancement of plant immunity.
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Claims 6
Claim · model-written summary
Peribacillus frigoritolerans AE95 promoted methanotroph growth in vitro.
- Taxon
- rice
Oryza sativat0 - Interactor
- Peribacillus frigoritolerans AE95Peribacillus frigoritoleranse0symbiont
- Outcome
- growth of methanotrophe0
- Direction
- promotinge0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified Peribacillus frigoritolerans AE95 as a rhizobacterium promoting the growth of methanotroph in vitro”
t0“rice”
Claim · model-written summary
Applying AE95 to rice roots reduced methane emission by up to 66.8 %.
- Taxon
- riceOryza sativae0
- Treatment
- Peribacillus frigoritolerans AE95e1
- Interactor
- Peribacillus frigoritolerans AE95Peribacillus frigoritoleranse1symbiont
- Outcome
- methane emissione0
- Direction
- reducinge0
- Value
- up to 66.8 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reducing methane emission by up to 66.8 % when applied to the rice root system in vivo”
e1“Peribacillus frigoritolerans AE95”
Claim · model-written summary
Metabolic modeling predicted l -alanine secretion as a growth-supporting metabolite.
- Taxon
- riceOryza sativat0
- Interactor
- P. frigoritolerans AE95Peribacillus frigoritoleranse0symbiont
- Compound
- l -alaninee0
- Outcome
- growth-supporting metabolitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genome-scale metabolic modelling predicted that l -alanine as a key growth-supporting metabolite secreted by P. frigoritolerans AE95”
t0“rice”
Claim · model-written summary
l -alanine enhanced Methylococcus geothermalis proliferation.
- Taxon
- riceOryza sativat0
- Treatment
- l -alaninee0
- Interactor
- Methylococcus geothermalisMethylococcus geothermalise0other
- Outcome
- Methylococcus geothermalis and Methylocystis parvus proliferatione0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“l -alanine as a key growth-supporting metabolite secreted by P. frigoritolerans AE95, which was experimentally validated to enhance Methylococcus geothermalis and Methylocystis parvus proliferation”
t0“rice”
Claim · model-written summary
P. frigoritolerans elicited rice innate immunity against Xanthomonas oryzae pv. oryzae.
- Taxon
- riceOryza sativae0
- Treatment
- P. frigoritoleranse0
- Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaee0pathogen - Second interactor
- P. frigoritoleransPeribacillus frigoritoleranse0symbiont
- Outcome
- rice innate immunitye0
- Direction
- elicitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. frigoritolerans and l -alanine supplementation elicited rice innate immunity against a bacterial pathogen Xanthomonas oryzae pv. oryzae”
Claim · model-written summary
l -alanine supplementation elicited rice innate immunity against Xanthomonas oryzae pv. oryzae.
- Taxon
- riceOryza sativae0
- Treatment
- l -alanine supplementatione0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Outcome
- rice innate immunitye0
- Direction
- elicitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. frigoritolerans and l -alanine supplementation elicited rice innate immunity against a bacterial pathogen Xanthomonas oryzae pv. oryzae”