Abstract
ABSTRACT The specific proteins from Japanese birch ( Betula platyphylla var. japonica plantlet No. 8) callus infected with a canker‐rot fungus Inonotus obliquus strain IO‐U1 (NBRC 113406) at the early infection stage were identified using proteome analysis. The intact, wounded, and infected calli were prepared, and the protein samples obtained at 2 days post‐treatments were subjected to two‐dimensional electrophoresis (2‐DE) for detecting infection‐specific proteins. The infection‐specific proteins were then analysed using LC/MS/MS, and the obtained data were subjected to the NCBIprot database for identifying the proteins based on homologous sequences search. A total of 134 protein spots on the 2‐DE gel were detected as infection‐specific proteins. Of these spots, 10 highly expressed proteins were identified as protochlorophyllide reductase chloroplastic (spot ID 1564), enolase (spot ID 368), mitochondrial‐processing peptidase subunit alpha‐like (spot ID 441), mitochondrial‐processing peptidase subunit alpha isoform B (spot ID 1578), T‐complex protein 1 subunit eta (spot ID 356), glutathione S ‐transferase‐like protein (spot ID 1121), glutathione S ‐transferase‐like protein, partial (spot ID 1527), protein WALLS ARE THIN 1‐like (spot ID 1444), and 1 Sc‐3 (spot IDs 1290 and 1322). The obtained results suggest that Japanese birch callus is capable of inducing several proteins related to photosynthesis, energy production, protein import machinery in mitochondria, protein folding, detoxification, secondary cell wall formation, and PR‐10 protein at the early infection stage as responses to defend itself against I. obliquus strain IO‐U1 infection.
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Claims 7
Claim · model-written summary
Proteome analysis detected 134 infection-specific protein spots in Japanese birch callus.
- Taxon
- Japanese birche1
- Material
- plantlet No. 8e1
- Organ / stage
- calluse1
- Interactor
- Inonotus obliquus strain IO‐U1 (NBRC 113406)
Inonotus obliquuse1pathogen - Outcome
- infection‐specific proteinse0
- Value
- 134 protein spotse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 134 protein spots on the 2‐DE gel were detected as infection‐specific proteins.”
e1“Japanese birch ( Betula platyphylla var. japonica plantlet No. 8) callus infected with a canker‐rot fungus Inonotus obliquus strain IO‐U1 (NBRC 113406)”
Claim · model-written summary
Highly expressed infection-specific proteins included protochlorophyllide reductase chloroplastic, enolase and mitochondrial-processing peptidase subunit alpha-like.
- Taxon
- Japanese birche1
- Organ / stage
- calluse1
- Interactor
- Inonotus obliquus strain IO‐U1 (NBRC 113406)Inonotus obliquuse1pathogen
- Outcome
- protochlorophyllide reductase chloroplastic (spot ID 1564), enolase (spot ID 368), mitochondrial‐processing peptidase subunit alpha‐like (spot ID 441)e0
- Qualifier
- 10 highly expressed proteinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10 highly expressed proteins were identified as protochlorophyllide reductase chloroplastic (spot ID 1564), enolase (spot ID 368), mitochondrial‐processing peptidase subunit alpha‐like (spot ID 441)”
e1“Japanese birch ( Betula platyphylla var. japonica plantlet No. 8) callus infected with a canker‐rot fungus Inonotus obliquus strain IO‐U1 (NBRC 113406)”
Claim · model-written summary
Identified infection-specific proteins included mitochondrial-processing peptidase subunit alpha isoform B, T-complex protein 1 subunit eta and glutathione S-transferase-like protein.
- Taxon
- Japanese birche1
- Organ / stage
- calluse1
- Interactor
- Inonotus obliquus strain IO‐U1 (NBRC 113406)Inonotus obliquuse1pathogen
- Outcome
- mitochondrial‐processing peptidase subunit alpha isoform B (spot ID 1578), T‐complex protein 1 subunit eta (spot ID 356), glutathione S ‐transferase‐like protein (spot ID 1121)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondrial‐processing peptidase subunit alpha isoform B (spot ID 1578), T‐complex protein 1 subunit eta (spot ID 356), glutathione S ‐transferase‐like protein (spot ID 1121)”
e1“Japanese birch ( Betula platyphylla var. japonica plantlet No. 8) callus infected with a canker‐rot fungus Inonotus obliquus strain IO‐U1 (NBRC 113406)”
Claim · model-written summary
Identified infection-specific proteins included partial glutathione S-transferase-like protein, WALLS ARE THIN 1-like and 1 Sc-3.
- Taxon
- Japanese birche1
- Organ / stage
- calluse1
- Interactor
- Inonotus obliquus strain IO‐U1 (NBRC 113406)Inonotus obliquuse1pathogen
- Outcome
- glutathione S ‐transferase‐like protein, partial (spot ID 1527), protein WALLS ARE THIN 1‐like (spot ID 1444), and 1 Sc‐3 (spot IDs 1290 and 1322)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glutathione S ‐transferase‐like protein, partial (spot ID 1527), protein WALLS ARE THIN 1‐like (spot ID 1444), and 1 Sc‐3 (spot IDs 1290 and 1322).”
e1“Japanese birch ( Betula platyphylla var. japonica plantlet No. 8) callus infected with a canker‐rot fungus Inonotus obliquus strain IO‐U1 (NBRC 113406)”
Claim · model-written summary
Early infected Japanese birch callus is interpreted as inducing defense proteins related to photosynthesis, energy production and mitochondrial protein import.
- Taxon
- Japanese birche0
- Organ / stage
- calluse0
- Interactor
- I. obliquus strain IO‐U1Inonotus obliquuse0pathogen
- Outcome
- inducing several proteinse0
- Qualifier
- photosynthesis, energy production, protein import machinery in mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The obtained results suggest that Japanese birch callus is capable of inducing several proteins related to photosynthesis, energy production, protein import machinery in mitochondria, protein folding, detoxification, secondary cell wall formation, and PR‐10 protein at the early infection stage as responses to defend itself against I. obliquus strain IO‐U1 infection.”
Claim · model-written summary
Early infected birch callus is interpreted as inducing defense proteins related to protein folding, detoxification and secondary cell wall formation.
- Taxon
- Japanese birche0
- Organ / stage
- calluse0
- Interactor
- I. obliquus strain IO‐U1Inonotus obliquuse0pathogen
- Outcome
- inducing several proteinse0
- Qualifier
- protein folding, detoxification, secondary cell wall formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The obtained results suggest that Japanese birch callus is capable of inducing several proteins related to photosynthesis, energy production, protein import machinery in mitochondria, protein folding, detoxification, secondary cell wall formation, and PR‐10 protein at the early infection stage as responses to defend itself against I. obliquus strain IO‐U1 infection.”
Claim · model-written summary
Early infected birch callus is interpreted as inducing PR-10 protein as a defense response.
- Taxon
- Japanese birche0
- Organ / stage
- calluse0
- Interactor
- I. obliquus strain IO‐U1Inonotus obliquuse0pathogen
- Gene
- PR‐10 proteine0
- Outcome
- inducing several proteinse0
- Qualifier
- early infection stagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The obtained results suggest that Japanese birch callus is capable of inducing several proteins related to photosynthesis, energy production, protein import machinery in mitochondria, protein folding, detoxification, secondary cell wall formation, and PR‐10 protein at the early infection stage as responses to defend itself against I. obliquus strain IO‐U1 infection.”