Abstract
Interactions between alfalfa and Verticillium albo-atrum were examined in terms of the following aspects:growth of V. albo-atrum on callus;growth of callus on the medium containing culture filtrate of V. albo-atrum and production of antifungal substances in callus treated with cell wall components (elicitor) from V. albo-atrum. Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning. The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited. Antifungal substances were detected in all cultivars treated with cell wall components, with concentrations being higher in resistant than in susceptible cultivars. These results suggest that antifungal activity in the callus extracts indicated by the inhibition of germ tube elongation of fungal conidia is the best criterion for the selection of resistant cultivars.
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Claims 9
Claim · model-written summary
Fungal mycelial growth was likely suppressed on Vertus callus with strong browning.
- Taxon
- V. albo-atrum
Verticillium albo-atrume0 - Material
- Vertuse0
- Interactor
- Alfalfa
Medicago sativat0host - Outcome
- Mycelial growthe0
- Direction
- suppressede0
- Qualifier
- likelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning.”
t0“Alfalfa Callus”
Claim · model-written summary
Fungal mycelial growth was likely suppressed on 5444 callus with strong browning.
- Taxon
- V. albo-atrumVerticillium albo-atrume0
- Material
- 5444e0
- Interactor
- AlfalfaMedicago sativat0host
- Outcome
- Mycelial growthe0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning.”
t0“Alfalfa Callus”
Claim · model-written summary
Vertus callus grew vigorously with 0.5 or 1% culture filtrate.
- Taxon
- AlfalfaMedicago sativat0
- Material
- Vertuse0
- Treatment
- 0.5 or 1% culture filtratee0
- Outcome
- growth of calluse0
- Direction
- vigorouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited.”
t0“Alfalfa Callus”
Claim · model-written summary
Kitawakaba callus grew vigorously with 0.5 or 1% culture filtrate.
- Taxon
- AlfalfaMedicago sativat0
- Material
- Kitawakabae0
- Treatment
- 0.5 or 1% culture filtratee0
- Outcome
- growth of calluse0
- Direction
- vigorouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited.”
t0“Alfalfa Callus”
Claim · model-written summary
5444 callus growth was inhibited by culture filtrate.
- Taxon
- AlfalfaMedicago sativat0
- Material
- 5444e0
- Treatment
- 0.5 or 1% culture filtratee0
- Outcome
- growth of calluse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited.”
t0“Alfalfa Callus”
Claim · model-written summary
Europe callus growth was inhibited by culture filtrate.
- Taxon
- AlfalfaMedicago sativat0
- Material
- Europee0
- Treatment
- 0.5 or 1% culture filtratee0
- Outcome
- growth of calluse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited.”
t0“Alfalfa Callus”
Claim · model-written summary
Antifungal substances were detected in all cultivars treated with cell wall components.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- cell wall componentse0
- Outcome
- Antifungal substancese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antifungal substances were detected in all cultivars treated with cell wall components, with concentrations being higher in resistant than in susceptible cultivars.”
t0“Alfalfa Callus”
Claim · model-written summary
Antifungal substance concentrations were higher in resistant than susceptible cultivars.
- Taxon
- AlfalfaMedicago sativat0
- Material
- resistante0
- Outcome
- concentrationse0
- Direction
- highere0
- Comparator
- susceptible cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antifungal substances were detected in all cultivars treated with cell wall components, with concentrations being higher in resistant than in susceptible cultivars.”
t0“Alfalfa Callus”
Claim · model-written summary
The authors propose callus extract antifungal activity as the best criterion for selecting resistant cultivars.
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- antifungal activity in the callus extractse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that antifungal activity in the callus extracts indicated by the inhibition of germ tube elongation of fungal conidia is the best criterion for the selection of resistant cultivars.”
t0“Alfalfa Callus”