Verticillium albo-atrum 24 claims

species · family Plectosphaerellaceae · fungus · NCBI Taxonomy ↗

NCBI synonyms: Acremonium apii · Verticillium alboatrum

2 spellings

Verticillium albo-atrumUSDA Forest Service - Northern and Intermountain Region · Public domain · Wikimedia Commons ↗

24 claims

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Observationobservedoriginal resultmeasured

Claim · model-written summary

Fungal transformants were obtained by co-cultivating A. tumefaciens with Verticillium albo-atrum on a range of wounded plant tissue types.

Taxon: Verticillium albo-atrumVerticillium albo-atrume1
Interactor: A. tumefaciensAgrobacterium tumefaciense1other
Outcome: Fungal transformantse2

Verbatim

e1
“A. tumefaciens harbouring the plasmid pCAMDsRed was co-cultivated with the common plant pathogenic fungus Verticillium albo-atrum on a range of wounded plant tissues”
✓ located verbatim in the source · abstract, characters 888–1053

e2
“Fungal transformants were obtained from co-cultivation on a range of plant tissue types”
✓ located verbatim in the source · abstract, characters 1055–1142

Title

Investigating Agrobacterium-Mediated Transformation of Verticillium albo-atrum on Plant Surfaces

Claire J. Knight; Andy M. Bailey; Gary D. Foster PLoS ONE 2010. doi:10.1371/journal.pone.0013684

Other claims from this paper (1)

Mechanism Plant tissue provides sufficient vir gene inducers to allow A. tumefaciens to transform fungi in planta.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Plant tissue provides sufficient vir gene inducers to allow A. tumefaciens to transform fungi in planta.

Taxon: Verticillium albo-atrumVerticillium albo-atrume1
Interactor: A. tumefaciensAgrobacterium tumefaciense1other
Outcome: transform fungi in plantae2
Qualifier: sufficient vir gene inducerse2

Verbatim

e1
“A. tumefaciens harbouring the plasmid pCAMDsRed was co-cultivated with the common plant pathogenic fungus Verticillium albo-atrum on a range of wounded plant tissues”
✓ located verbatim in the source · abstract, characters 888–1053

e2
“plant tissue provides sufficient vir gene inducers to allow A. tumefaciens to transform fungi in planta”
✓ located verbatim in the source · abstract, characters 1163–1266

Title

Investigating Agrobacterium-Mediated Transformation of Verticillium albo-atrum on Plant Surfaces

Claire J. Knight; Andy M. Bailey; Gary D. Foster PLoS ONE 2010. doi:10.1371/journal.pone.0013684

Other claims from this paper (1)

Observation Fungal transformants were obtained by co-cultivating A. tumefaciens with Verticillium albo-atrum on a range of wounded plant tissue types.

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Fungal mycelial growth was likely suppressed on Vertus callus with strong browning.

Taxon: V. albo-atrumVerticillium albo-atrume0
Material: Vertuse0
Interactor: AlfalfaMedicago sativat0host
Outcome: Mycelial growthe0
Direction: suppressede0
Qualifier: likelye0

Verbatim

e0
“Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning.”
✓ located verbatim in the source · abstract, characters 330–480

t0
“Alfalfa Callus”
✓ located verbatim in the source · title, characters 69–83

Title

Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components

Masanori Koike; Kouhei Nanbu; Tohru Shimada Institutional Repositories DataBase (IRDB) 1992. doi:10.14941/grass.37.412

Other claims from this paper (8)

Observation Fungal mycelial growth was likely suppressed on 5444 callus with strong browning.
Intervention effect Vertus callus grew vigorously with 0.5 or 1% culture filtrate.
Intervention effect Kitawakaba callus grew vigorously with 0.5 or 1% culture filtrate.
Intervention effect 5444 callus growth was inhibited by culture filtrate.
Intervention effect Europe callus growth was inhibited by culture filtrate.
Observation Antifungal substances were detected in all cultivars treated with cell wall components.
Association Antifungal substance concentrations were higher in resistant than susceptible cultivars.
Recommendation The authors propose callus extract antifungal activity as the best criterion for selecting resistant cultivars.

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Fungal mycelial growth was likely suppressed on 5444 callus with strong browning.

Taxon: V. albo-atrumVerticillium albo-atrume0
Material: 5444e0
Interactor: AlfalfaMedicago sativat0host
Outcome: Mycelial growthe0
Direction: suppressede0

Verbatim

e0
“Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning.”
✓ located verbatim in the source · abstract, characters 330–480

t0
“Alfalfa Callus”
✓ located verbatim in the source · title, characters 69–83

Title

Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components

Masanori Koike; Kouhei Nanbu; Tohru Shimada Institutional Repositories DataBase (IRDB) 1992. doi:10.14941/grass.37.412

Other claims from this paper (8)

Observation Fungal mycelial growth was likely suppressed on Vertus callus with strong browning.
Intervention effect Vertus callus grew vigorously with 0.5 or 1% culture filtrate.
Intervention effect Kitawakaba callus grew vigorously with 0.5 or 1% culture filtrate.
Intervention effect 5444 callus growth was inhibited by culture filtrate.
Intervention effect Europe callus growth was inhibited by culture filtrate.
Observation Antifungal substances were detected in all cultivars treated with cell wall components.
Association Antifungal substance concentrations were higher in resistant than susceptible cultivars.
Recommendation The authors propose callus extract antifungal activity as the best criterion for selecting resistant cultivars.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The exopolygalacturonase had molecular weight 44 000.

Taxon: Verticillium albo-atrumVerticillium albo-atrumt0
Gene: exopolygalacturonasee0
Outcome: molecular weighte0
Value: 44 000e0

Not reportedDirection

Verbatim

e0
“The exopolygalacturonase had a molecular weight of 44 000, pI of 6.5, and pH optima of 4.5 for pectin and 5.25 for sodium polypectate.”
✓ located verbatim in the source · abstract, characters 339–473

t0
“Verticillium albo-atrum”
✓ located verbatim in the source · title, characters 55–78

Title

Characterization of Two Polygalacturonases Produced by Verticillium albo-atrum

H. W. Mussell; Blanche Strouse Canadian Journal of Biochemistry 1972. doi:10.1139/o72-086

Other claims from this paper (10)

Observation The exopolygalacturonase had pI 6.5.
Observation Exopolygalacturonase pH optimum was 4.5 for pectin.
Observation Exopolygalacturonase pH optimum was 5.25 for sodium polypectate.
Observation Endopolygalacturonase molecular weight was 34 500.
Observation Endopolygalacturonase pI was 9.7.
Observation Endopolygalacturonase pH optimum was 5.0 for pectin.
Observation Endopolygalacturonase pH optimum was 6.0 for sodium polypectate.
Observation Purified exopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Observation Purified endopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Intervention effect Endo-enzyme macerated plant tissue 20 times as effectively as exo-enzyme.

Observationobservedoriginal resultmeasured

Claim · model-written summary

The exopolygalacturonase had pI 6.5.

Taxon: Verticillium albo-atrumVerticillium albo-atrumt0
Gene: exopolygalacturonasee0
Outcome: pIe0
Value: 6.5e0

Not reportedDirection

Verbatim

e0
“The exopolygalacturonase had a molecular weight of 44 000, pI of 6.5”
✓ located verbatim in the source · abstract, characters 339–407

t0
“Verticillium albo-atrum”
✓ located verbatim in the source · title, characters 55–78

Title

Characterization of Two Polygalacturonases Produced by Verticillium albo-atrum

H. W. Mussell; Blanche Strouse Canadian Journal of Biochemistry 1972. doi:10.1139/o72-086

Other claims from this paper (10)

Observation The exopolygalacturonase had molecular weight 44 000.
Observation Exopolygalacturonase pH optimum was 4.5 for pectin.
Observation Exopolygalacturonase pH optimum was 5.25 for sodium polypectate.
Observation Endopolygalacturonase molecular weight was 34 500.
Observation Endopolygalacturonase pI was 9.7.
Observation Endopolygalacturonase pH optimum was 5.0 for pectin.
Observation Endopolygalacturonase pH optimum was 6.0 for sodium polypectate.
Observation Purified exopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Observation Purified endopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Intervention effect Endo-enzyme macerated plant tissue 20 times as effectively as exo-enzyme.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Exopolygalacturonase pH optimum was 4.5 for pectin.

Taxon: Verticillium albo-atrumVerticillium albo-atrumt0
Compound: pectine0
Outcome: pH optimae0
Value: 4.5e0

Not reportedDirection

Verbatim

e0
“The exopolygalacturonase had a molecular weight of 44 000, pI of 6.5, and pH optima of 4.5 for pectin”
✓ located verbatim in the source · abstract, characters 339–440

t0
“Verticillium albo-atrum”
✓ located verbatim in the source · title, characters 55–78

Title

Characterization of Two Polygalacturonases Produced by Verticillium albo-atrum

H. W. Mussell; Blanche Strouse Canadian Journal of Biochemistry 1972. doi:10.1139/o72-086

Other claims from this paper (10)

Observation The exopolygalacturonase had molecular weight 44 000.
Observation The exopolygalacturonase had pI 6.5.
Observation Exopolygalacturonase pH optimum was 5.25 for sodium polypectate.
Observation Endopolygalacturonase molecular weight was 34 500.
Observation Endopolygalacturonase pI was 9.7.
Observation Endopolygalacturonase pH optimum was 5.0 for pectin.
Observation Endopolygalacturonase pH optimum was 6.0 for sodium polypectate.
Observation Purified exopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Observation Purified endopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Intervention effect Endo-enzyme macerated plant tissue 20 times as effectively as exo-enzyme.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Exopolygalacturonase pH optimum was 5.25 for sodium polypectate.

Taxon: Verticillium albo-atrumVerticillium albo-atrumt0
Compound: sodium polypectatee0
Outcome: pH optimae0
Value: 5.25e0

Not reportedDirection

Verbatim

e0
“The exopolygalacturonase had a molecular weight of 44 000, pI of 6.5, and pH optima of 4.5 for pectin and 5.25 for sodium polypectate.”
✓ located verbatim in the source · abstract, characters 339–473

t0
“Verticillium albo-atrum”
✓ located verbatim in the source · title, characters 55–78

Title

Characterization of Two Polygalacturonases Produced by Verticillium albo-atrum

H. W. Mussell; Blanche Strouse Canadian Journal of Biochemistry 1972. doi:10.1139/o72-086

Other claims from this paper (10)

Observation The exopolygalacturonase had molecular weight 44 000.
Observation The exopolygalacturonase had pI 6.5.
Observation Exopolygalacturonase pH optimum was 4.5 for pectin.
Observation Endopolygalacturonase molecular weight was 34 500.
Observation Endopolygalacturonase pI was 9.7.
Observation Endopolygalacturonase pH optimum was 5.0 for pectin.
Observation Endopolygalacturonase pH optimum was 6.0 for sodium polypectate.
Observation Purified exopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Observation Purified endopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Intervention effect Endo-enzyme macerated plant tissue 20 times as effectively as exo-enzyme.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Endopolygalacturonase molecular weight was 34 500.

Taxon: Verticillium albo-atrumVerticillium albo-atrumt0
Gene: endopolygalacturonasee0
Outcome: molecular weighte0
Value: 34 500e0

Not reportedDirection

Verbatim

e0
“The endopolygalacturonase had a molecular weight of 34 500, pI of 9.7, and pH optima of 5.0 for pectin and 6.0 for sodium polypectate.”
✓ located verbatim in the source · abstract, characters 474–608

t0
“Verticillium albo-atrum”
✓ located verbatim in the source · title, characters 55–78

Title

Characterization of Two Polygalacturonases Produced by Verticillium albo-atrum

H. W. Mussell; Blanche Strouse Canadian Journal of Biochemistry 1972. doi:10.1139/o72-086

Other claims from this paper (10)

Observation The exopolygalacturonase had molecular weight 44 000.
Observation The exopolygalacturonase had pI 6.5.
Observation Exopolygalacturonase pH optimum was 4.5 for pectin.
Observation Exopolygalacturonase pH optimum was 5.25 for sodium polypectate.
Observation Endopolygalacturonase pI was 9.7.
Observation Endopolygalacturonase pH optimum was 5.0 for pectin.
Observation Endopolygalacturonase pH optimum was 6.0 for sodium polypectate.
Observation Purified exopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Observation Purified endopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Intervention effect Endo-enzyme macerated plant tissue 20 times as effectively as exo-enzyme.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Endopolygalacturonase pI was 9.7.

Taxon: Verticillium albo-atrumVerticillium albo-atrumt0
Outcome: pIe0
Value: 9.7e0

Not reportedDirection

Verbatim

e0
“The endopolygalacturonase had a molecular weight of 34 500, pI of 9.7”
✓ located verbatim in the source · abstract, characters 474–543

t0
“Verticillium albo-atrum”
✓ located verbatim in the source · title, characters 55–78

Title

Characterization of Two Polygalacturonases Produced by Verticillium albo-atrum

H. W. Mussell; Blanche Strouse Canadian Journal of Biochemistry 1972. doi:10.1139/o72-086

Other claims from this paper (10)

Observation The exopolygalacturonase had molecular weight 44 000.
Observation The exopolygalacturonase had pI 6.5.
Observation Exopolygalacturonase pH optimum was 4.5 for pectin.
Observation Exopolygalacturonase pH optimum was 5.25 for sodium polypectate.
Observation Endopolygalacturonase molecular weight was 34 500.
Observation Endopolygalacturonase pH optimum was 5.0 for pectin.
Observation Endopolygalacturonase pH optimum was 6.0 for sodium polypectate.
Observation Purified exopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Observation Purified endopolygalacturonase was electrophoretically homogeneous and exceptionally stable in solution at 4°.
Intervention effect Endo-enzyme macerated plant tissue 20 times as effectively as exo-enzyme.