Paper · 2018 · RePEc: Research Papers in Economics

Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis

Gabrielen Dias; David Dos Anjos; Bárbara Nogueira De Souza; et al.

5
claims
11
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Adding silicon to the culture medium may contribute, to improve many growth parameters including embryogenesis and organogenesis. The objective of this study was to evaluate the embryogenic potential of passion fruit in vitro (Passiflora edulis f. Flavicarpa O. Deg), submitted to different concentrations of silicon. Nodal segments of plants were inoculated at five concentrations of silicic acid added to the culture medium, 0.0; 0.5; 1.0; 1.5 and 2.0 g L-1. After 60 days, the visual characteristics (% contamination, callus, bud, root and oxidation), cytochemical test and microanalysis of X-rays evaluated. Concentrations of 0.5; 1.0 and 2.0 g L-1 of silicon did not differ in relation to callus formation. By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine and, little reaction to the Evans blue associated with cell death. By the microanalysis of X-rays, the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L-1 of silicon in comparison to the other concentrations. The silicon added to the culture medium has a response in the formation of cells with embryogenic potential in nodal segments of passion fruit. The concentration of 2.0 g L-1 of silicon acid (H4SiO4) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg.

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Claims 5

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Silicon concentrations of 0.5, 1.0 and 2.0 g/L did not differ in callus formation of Passiflora edulis in vitro.

Taxon
Passiflora edulisPassiflora edulist0
Organ / stage
calluse1
Treatment
0.5; 1.0 and 2.0 g L-1 of silicone1
Outcome
callus formatione1
Direction
did not differe1
Setting
in vitrot0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Passiflora edulis
Passiflora edulis · TaxonRarani · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis”

✓ located verbatim in the source · title, characters 0–76 · show in text ↑
e1

“Concentrations of 0.5; 1.0 and 2.0 g L-1 of silicon did not differ in relation to callus formation”

✓ located verbatim in the source · abstract, characters 612–710 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

All silicon treatments showed embryogenic cells in the cytochemical test in Passiflora edulis callus.

Taxon
Passiflora edulisPassiflora edulist0
Treatment
all treatmentse2
Outcome
embryogenic cellse2

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Passiflora edulis
Passiflora edulis · TaxonRarani · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis”

✓ located verbatim in the source · title, characters 0–76 · show in text ↑
e2

“By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine”

✓ located verbatim in the source · abstract, characters 712–824 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus from all silicon treatments showed little reaction to Evans blue, which is associated with cell death.

Taxon
Passiflora edulisPassiflora edulist0
Treatment
all treatmentse2
Outcome
reaction to the Evans bluee3
Direction
littlee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Passiflora edulis
Passiflora edulis · TaxonRarani · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis”

✓ located verbatim in the source · title, characters 0–76 · show in text ↑
e2

“By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine”

✓ located verbatim in the source · abstract, characters 712–824 · show in text ↑
e3

“little reaction to the Evans blue associated with cell death”

✓ located verbatim in the source · abstract, characters 830–890 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

At 1.5 g/L silicon, sulfur and phosphorus absorption responses were significant compared with the other concentrations in Passiflora edulis.

Taxon
Passiflora edulisPassiflora edulist0
Treatment
1.5 g L-1 of silicone4
Compound
sulfur and phosphoruse4
Outcome
significant absorption responsese4
Comparator
other concentrationse4

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Passiflora edulis
Passiflora edulis · TaxonRarani · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis”

✓ located verbatim in the source · title, characters 0–76 · show in text ↑
e4

“the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L-1 of silicon in comparison to the other concentrations”

✓ located verbatim in the source · abstract, characters 924–1090 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 2.0 g/L silicon concentration gave a high embryogenic potential in Passiflora edulis cells.

Taxon
Passiflora edulisPassiflora edulist0
Treatment
2.0 g L-1 of silicon acid (H4SiO4)e5
Outcome
embryogenic potentiale5
Direction
highe5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Passiflora edulis
Passiflora edulis · TaxonRarani · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis”

✓ located verbatim in the source · title, characters 0–76 · show in text ↑
e5

“The concentration of 2.0 g L-1 of silicon acid (H4SiO4) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg”

✓ located verbatim in the source · abstract, characters 1236–1387 · show in text ↑

silicon atom · growth · Passiflora edulis (passion fruit)Positive and negative results

8 papers: 6 positive, 2 negative or no effect

1 paper in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.