Abstract
Emerging evidence suggests that multiple RNA recognition motif (RRM)–containing proteins are involved in transcriptional regulation, yet the underlying mechanisms remain largely unclear. Here, we show that the previously uncharacterized LATE-FLOWERING RRM-CONTAINING PROTEIN (LRP) prevents 5′-3′ chromatin looping of the key floral repressor gene FLOWERING LOCUS C ( FLC ) via direct association with its 5′ and 3′ genomic regions. Loss of LRP function leads to increased 5′-3′ gene looping, reduced RNA polymerase II (Pol II) occupancy at the FLC locus, thereby increasing FLC transcription and delaying flowering in Arabidopsis thaliana . Furthermore, site-specific phosphorylation of LRP at serine 17, mediated by the kinase PRP4KA, is essential for LRP protein stability and its function in preventing FLC chromatin looping and Pol II recruitment. Our findings reveal that site-specific phosphorylation of LRP by PRP4KA precludes FLC gene loop formation, constituting a key mechanism for FLC transcriptional repression to ensure timely flowering.
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Claims 8
Claim · model-written summary
LRP prevents FLC chromatin looping through association with the 5′ and 3′ genomic regions.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- LATE-FLOWERING RRM-CONTAINING PROTEIN (LRP)e0
- Outcome
- 5′-3′ chromatin loopinge0
- Direction
- preventse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LATE-FLOWERING RRM-CONTAINING PROTEIN (LRP) prevents 5′-3′ chromatin looping of the key floral repressor gene FLOWERING LOCUS C ( FLC ) via direct association with its 5′ and 3′ genomic regions”
e1“Arabidopsis thaliana”
Claim · model-written summary
Loss of LRP function increased FLC gene looping.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of LRP functione0
- Gene
- LRPe0
- Outcome
- 5′-3′ gene loopinge0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of LRP function leads to increased 5′-3′ gene looping, reduced RNA polymerase II (Pol II) occupancy at the FLC locus, thereby increasing FLC transcription and delaying flowering in Arabidopsis thaliana”
Claim · model-written summary
Loss of LRP function reduced RNA polymerase II occupancy at FLC.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of LRP functione0
- Gene
- LRPe0
- Outcome
- RNA polymerase II (Pol II) occupancy at the FLC locuse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of LRP function leads to increased 5′-3′ gene looping, reduced RNA polymerase II (Pol II) occupancy at the FLC locus, thereby increasing FLC transcription and delaying flowering in Arabidopsis thaliana”
Claim · model-written summary
Loss of LRP function increased FLC transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of LRP functione0
- Gene
- FLCe0
- Outcome
- FLC transcriptione0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of LRP function leads to increased 5′-3′ gene looping, reduced RNA polymerase II (Pol II) occupancy at the FLC locus, thereby increasing FLC transcription and delaying flowering in Arabidopsis thaliana”
Claim · model-written summary
Loss of LRP function delayed flowering.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Loss of LRP functione0
- Gene
- LRPe0
- Outcome
- floweringe0
- Direction
- delayinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of LRP function leads to increased 5′-3′ gene looping, reduced RNA polymerase II (Pol II) occupancy at the FLC locus, thereby increasing FLC transcription and delaying flowering in Arabidopsis thaliana”
Claim · model-written summary
PRP4KA mediates LRP phosphorylation at serine 17, which is essential for LRP protein stability.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- PRP4KAe0
- Outcome
- LRP protein stabilitye0
- Qualifier
- site-specific phosphorylation of LRP at serine 17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“site-specific phosphorylation of LRP at serine 17, mediated by the kinase PRP4KA, is essential for LRP protein stability”
e1“Arabidopsis thaliana”
Claim · model-written summary
PRP4KA-mediated phosphorylation of LRP at serine 17 is essential to prevent FLC chromatin looping.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- LRPe0
- Outcome
- FLC chromatin loopinge0
- Direction
- preventinge0
- Qualifier
- phosphorylation of LRP at serine 17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“site-specific phosphorylation of LRP at serine 17, mediated by the kinase PRP4KA, is essential for LRP protein stability and its function in preventing FLC chromatin looping and Pol II recruitment”
e1“Arabidopsis thaliana”
Claim · model-written summary
PRP4KA-mediated phosphorylation of LRP at serine 17 is essential for its function in preventing Pol II recruitment.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- LRPe0
- Outcome
- Pol II recruitmente0
- Direction
- preventinge0
- Qualifier
- phosphorylation of LRP at serine 17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“site-specific phosphorylation of LRP at serine 17, mediated by the kinase PRP4KA, is essential for LRP protein stability and its function in preventing FLC chromatin looping and Pol II recruitment”
e1“Arabidopsis thaliana”