Paper · 2026 · Horticulturae

Variability and Permanency: Variation in the Density of Leaf Glandular Trichomes and Terpene Composition in Mentha spicata var. crispa (Benth.) Danert and M. × piperita var. citrata (Ehrh.) Briq.

Anna Vladimirovna Shirokova; Maria Sergeevna Plykina; Alexander Olegovich Ruzhitskiy; et al.

10
claims
18
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by

Abstract

Essential oils (EOs) of Mentha spicata var. crispa (Benth.) Danert and M. × piperita var. citrata (Ehrh.) Briq. and EO components are widely used in medicine, pharmaceuticals, cosmetics, hygiene products, the food industry, and other fields, and have a high commercial value. The variety Mentha spicata var. crispa is also used as an ornamental plant due to its distinctive curled leaves. Studying the influence of growing conditions and harvest timing on EO yield and the major compound concentrations is one of the key research directions for Mentha species, aimed at the ascertainment of the ways of increasing EO production and quality. Gas chromatography analysis of the component composition of EOs from leaves of Mentha spicata var. crispa “Kurchavaya” (MscK) showed that it remained stable both in July and September, with carvone predominating (81% and 85%, respectively). In contrast, the EO composition from M. × piperita var. citrata “Apelsinovaya” (MpcA) leaves changed in the course of the vegetation period. In July, menthofuran dominated (30%), while in September, linalool and its acetate were predominant (34% and 47%, respectively), which was typical for this chemotype. At the same time, the content of EOs and the density of glandular trichomes (GTs) (the OE storage sites) in MscK were higher in July and decreased by September, whereas in MpcA, both EO content and the number of GTs increased from July to September. These changes may have been caused by temperature fluctuations. Thus, MscK proved to be more resistant to environmental factors than MpcA.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

The essential oil composition of Mentha spicata var. crispa Kurchavaya remained stable in July and September, with carvone predominating.

Taxon
Mentha spicata var. crispaMentha spicatae0
Material
Kurchavayae0
Organ / stage
leavese0
Outcome
component composition of EOse0
Value
81% and 85%e0
Qualifier
remained stable both in July and September, with carvone predominatinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha spicata
Mentha spicata · TaxonSimon Eugster -- Simon 13:07, 2 July 2006 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Gas chromatography analysis of the component composition of EOs from leaves of Mentha spicata var. crispa “Kurchavaya” (MscK) showed that it remained stable both in July and September, with carvone predominating (81% and 85%, respectively)”

✓ located verbatim in the source · abstract, characters 641–880 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The essential oil composition of M. × piperita var. citrata Apelsinovaya leaves changed over the vegetation period.

Taxon
M. × piperita var. citrataMentha aquatica var. citratae1
Material
Apelsinovayae1
Organ / stage
leavese1
Outcome
EO compositione1
Qualifier
changed in the course of the vegetation periode1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha aquatica var. citrata
Mentha aquatica var. citrata · Taxon · picture of the speciesKristian Peters -- Fabelfroh 09:40, 12 March 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“In contrast, the EO composition from M. × piperita var. citrata “Apelsinovaya” (MpcA) leaves changed in the course of the vegetation period”

✓ located verbatim in the source · abstract, characters 882–1021 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In July, menthofuran dominated the essential oil of M. × piperita var. citrata (30%).

Taxon
M. × piperita var. citrataMentha aquatica var. citratae1
Material
Apelsinovayae1
Outcome
menthofuran dominatede2
Value
30%e2
Setting
Julye2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha aquatica var. citrata
Mentha aquatica var. citrata · Taxon · picture of the speciesKristian Peters -- Fabelfroh 09:40, 12 March 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“In contrast, the EO composition from M. × piperita var. citrata “Apelsinovaya” (MpcA) leaves changed in the course of the vegetation period”

✓ located verbatim in the source · abstract, characters 882–1021 · show in text ↑
e2

“In July, menthofuran dominated (30%), while in September, linalool and its acetate were predominant (34% and 47%, respectively), which was typical for this chemotype”

✓ located verbatim in the source · abstract, characters 1023–1188 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In September, linalool and its acetate were predominant in the essential oil of M. × piperita var. citrata (34% and 47%).

Taxon
M. × piperita var. citrataMentha aquatica var. citratae1
Material
Apelsinovayae1
Outcome
linalool and its acetate were predominante2
Value
34% and 47%e2
Setting
Septembere2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha aquatica var. citrata
Mentha aquatica var. citrata · Taxon · picture of the speciesKristian Peters -- Fabelfroh 09:40, 12 March 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“In contrast, the EO composition from M. × piperita var. citrata “Apelsinovaya” (MpcA) leaves changed in the course of the vegetation period”

✓ located verbatim in the source · abstract, characters 882–1021 · show in text ↑
e2

“In July, menthofuran dominated (30%), while in September, linalool and its acetate were predominant (34% and 47%, respectively), which was typical for this chemotype”

✓ located verbatim in the source · abstract, characters 1023–1188 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Essential oil content of MscK was higher in July and decreased by September.

Taxon
Mentha spicata var. crispaMentha spicatae0
Material
MscKe3
Outcome
content of EOse3
Direction
highere3
Qualifier
decreased by Septembere3
Setting
Julye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha spicata
Mentha spicata · TaxonSimon Eugster -- Simon 13:07, 2 July 2006 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Gas chromatography analysis of the component composition of EOs from leaves of Mentha spicata var. crispa “Kurchavaya” (MscK) showed that it remained stable both in July and September, with carvone predominating (81% and 85%, respectively)”

✓ located verbatim in the source · abstract, characters 641–880 · show in text ↑
e3

“At the same time, the content of EOs and the density of glandular trichomes (GTs) (the OE storage sites) in MscK were higher in July and decreased by September, whereas in MpcA, both EO content and the number of GTs increased from July to September”

✓ located verbatim in the source · abstract, characters 1190–1438 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Glandular trichome density of MscK was higher in July and decreased by September.

Taxon
Mentha spicata var. crispaMentha spicatae0
Material
MscKe3
Outcome
density of glandular trichomes (GTs)e3
Direction
highere3
Qualifier
decreased by Septembere3
Setting
Julye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha spicata
Mentha spicata · TaxonSimon Eugster -- Simon 13:07, 2 July 2006 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Gas chromatography analysis of the component composition of EOs from leaves of Mentha spicata var. crispa “Kurchavaya” (MscK) showed that it remained stable both in July and September, with carvone predominating (81% and 85%, respectively)”

✓ located verbatim in the source · abstract, characters 641–880 · show in text ↑
e3

“At the same time, the content of EOs and the density of glandular trichomes (GTs) (the OE storage sites) in MscK were higher in July and decreased by September, whereas in MpcA, both EO content and the number of GTs increased from July to September”

✓ located verbatim in the source · abstract, characters 1190–1438 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Essential oil content of MpcA increased from July to September.

Taxon
M. × piperita var. citrataMentha aquatica var. citratae1
Material
MpcAe3
Outcome
EO contente3
Direction
increasede3
Qualifier
from July to Septembere3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha aquatica var. citrata
Mentha aquatica var. citrata · Taxon · picture of the speciesKristian Peters -- Fabelfroh 09:40, 12 March 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“In contrast, the EO composition from M. × piperita var. citrata “Apelsinovaya” (MpcA) leaves changed in the course of the vegetation period”

✓ located verbatim in the source · abstract, characters 882–1021 · show in text ↑
e3

“At the same time, the content of EOs and the density of glandular trichomes (GTs) (the OE storage sites) in MscK were higher in July and decreased by September, whereas in MpcA, both EO content and the number of GTs increased from July to September”

✓ located verbatim in the source · abstract, characters 1190–1438 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The number of glandular trichomes of MpcA increased from July to September.

Taxon
M. × piperita var. citrataMentha aquatica var. citratae1
Material
MpcAe3
Outcome
the number of GTse3
Direction
increasede3
Qualifier
from July to Septembere3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha aquatica var. citrata
Mentha aquatica var. citrata · Taxon · picture of the speciesKristian Peters -- Fabelfroh 09:40, 12 March 2007 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“In contrast, the EO composition from M. × piperita var. citrata “Apelsinovaya” (MpcA) leaves changed in the course of the vegetation period”

✓ located verbatim in the source · abstract, characters 882–1021 · show in text ↑
e3

“At the same time, the content of EOs and the density of glandular trichomes (GTs) (the OE storage sites) in MscK were higher in July and decreased by September, whereas in MpcA, both EO content and the number of GTs increased from July to September”

✓ located verbatim in the source · abstract, characters 1190–1438 · show in text ↑
Mechanismhypothesisoriginal resultmeasured

Claim · model-written summary

Essential oil composition changes in these Mentha varieties may have been caused by temperature fluctuations.

Taxon
Mentha spicata var. crispaMentha spicatae0
Outcome
changese4
Qualifier
temperature fluctuationse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha spicata
Mentha spicata · TaxonSimon Eugster -- Simon 13:07, 2 July 2006 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Gas chromatography analysis of the component composition of EOs from leaves of Mentha spicata var. crispa “Kurchavaya” (MscK) showed that it remained stable both in July and September, with carvone predominating (81% and 85%, respectively)”

✓ located verbatim in the source · abstract, characters 641–880 · show in text ↑
e4

“These changes may have been caused by temperature fluctuations”

✓ located verbatim in the source · abstract, characters 1440–1502 · show in text ↑
Associationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

MscK proved more resistant to environmental factors than MpcA.

Taxon
Mentha spicata var. crispaMentha spicatae0
Material
MscKe5
Outcome
resistant to environmental factorse5
Direction
moree5
Comparator
MpcAe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mentha spicata
Mentha spicata · TaxonSimon Eugster -- Simon 13:07, 2 July 2006 (UTC) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Gas chromatography analysis of the component composition of EOs from leaves of Mentha spicata var. crispa “Kurchavaya” (MscK) showed that it remained stable both in July and September, with carvone predominating (81% and 85%, respectively)”

✓ located verbatim in the source · abstract, characters 641–880 · show in text ↑
e5

“Thus, MscK proved to be more resistant to environmental factors than MpcA”

✓ located verbatim in the source · abstract, characters 1504–1577 · show in text ↑