Abstract
Botrytis cinerea Pers., the causal agent of grey mould, causes major economic losses in viticulture by reducing grape and wine quality and yield. Antagonistic yeasts that release bioactive volatile organic compounds (VOCs) represent a sustainable alternative to synthetic fungicides. Here, VOCs produced by Pichia guilliermondii strain ZIM624 were identified and assessed for antifungal activity against B. cinerea. 65 VOCs—including higher alcohols, volatile phenols, esters, and terpenes—were detected using two newly developed and validated analytical methods combining automated headspace solid-phase microextraction with gas chromatography–mass spectrometry. A total of 13 VOCs were selected for the bioassays. Fumigation assays demonstrated that terpenes (citronellol, geraniol, nerol, α-terpineol, and linalool) were the most effective inhibitors of B. cinerea mycelial growth (EC50 = 6.3–33.9 μL/L). Strong inhibition was also observed for 4-vinylphenol and isoamyl acetate. In vivo assays confirmed that exposing infected grape berries to P. guilliermondii VOCs significantly reduced grey mould incidence. These results highlight the potential of P. guilliermondii ZIM624 volatiles as natural biofumigants for the eco-friendly management of B. cinerea in grapes. Future research should focus on optimising VOC production, evaluating efficacy under field conditions, and developing formulations for practical application in vineyards and post-harvest storage. Additionally, investigating potential synergistic effects of VOC combinations could lead to more effective biocontrol strategies.
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Claims 6
Claim · model-written summary
Two analytical methods detected 65 volatiles from Pichia guilliermondii ZIM624.
Verbatim
e0“65 VOCs—including higher alcohols, volatile phenols, esters, and terpenes—were detected using two newly developed and validated analytical methods combining automated headspace solid-phase microextraction with gas chromatography–mass spectrometry”
t0“Grape-Based Systems”
Claim · model-written summary
The tested terpenes were the strongest inhibitors of Botrytis cinerea mycelial growth.
- Taxon
- Grapet0
- Treatment
- terpenese0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- B. cinerea mycelial growthe0
- Direction
- most effective inhibitorse0
- Value
- 6.3–33.9 μL/Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“terpenes (citronellol, geraniol, nerol, α-terpineol, and linalool) were the most effective inhibitors of B. cinerea mycelial growth (EC50 = 6.3–33.9 μL/L)”
t0“Grape-Based Systems”
Claim · model-written summary
4-vinylphenol strongly inhibited Botrytis cinerea.
- Taxon
- Grapet0
- Treatment
- 4-vinylphenole0
- Interactor
- Botrytis cinereaBotrytis cinereat0pathogen
- Outcome
- inhibitione0
- Direction
- Strong inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong inhibition was also observed for 4-vinylphenol and isoamyl acetate.”
t0“Botrytis cinerea in Grape-Based Systems”
Claim · model-written summary
Isoamyl acetate strongly inhibited Botrytis cinerea.
- Taxon
- Grapet0
- Treatment
- isoamyl acetatee0
- Interactor
- Botrytis cinereaBotrytis cinereat0pathogen
- Outcome
- inhibitione0
- Direction
- Strong inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong inhibition was also observed for 4-vinylphenol and isoamyl acetate.”
t0“Botrytis cinerea in Grape-Based Systems”
Claim · model-written summary
Exposure to P. guilliermondii VOCs significantly reduced grey mould incidence in infected grape berries.
- Taxon
- grapee0
- Treatment
- exposing infected grape berries to P. guilliermondii VOCse0
- Interactor
- Botrytis cinereaBotrytis cinereat0pathogen
- Second interactor
- P. guilliermondiie0biocontrol_agent
- Outcome
- grey mould incidencee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exposing infected grape berries to P. guilliermondii VOCs significantly reduced grey mould incidence”
e1“B. cinerea mycelial growth”
t0“Botrytis cinerea in Grape-Based Systems”
Claim · model-written summary
The authors propose P. guilliermondii ZIM624 volatiles as biofumigants for grey mould management in grapes.
- Taxon
- grapese0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Second interactor
- P. guilliermondii ZIM624e0biocontrol_agent
- Outcome
- natural biofumigants for the eco-friendly management of B. cinerea in grapese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potential of P. guilliermondii ZIM624 volatiles as natural biofumigants for the eco-friendly management of B. cinerea in grapes”
The same questions in other papers
volatile organic compound · Botrytis cinerea · VitisResults agree
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1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.