Abstract
Zinc (Zn) is a mineral micronutrient that is essential for plant growth and development. Soil Zn deficiency or excess severely impacts plant health and crop yields. MicroRNAs (miRNAs) play crucial roles in plant responses to abiotic stress, but their roles in Zn homeostasis in important crop bread wheat (Triticum aestivum L.) remain unknown. This study investigated miRNA expression profiles in wheat roots under different Zn supply conditions using high-throughput sequencing. Phenotypic and physiological analyses revealed that high Zn promoted wheat plant growth, while low and excess Zn resulted in wheat plant growth inhibition and oxidative stress. A total of 798 miRNAs (including 70 known and 728 novel miRNAs) were identified; among them, 10 known and 122 novel miRNAs were differentially expressed. Many key miRNAs, such as miR397-5p, miR398, 4D_25791, and 5A_27668, are up-regulated under low Zn but down-regulated under high Zn and excess Zn. Target gene prediction and enrichment analysis revealed that the regulated genes of these miRNAs focused on “zinc ion transmembrane transporter activity”, “divalent inorganic cation transmembrane transporter activity”, and “cellular detoxification” processes in the low Zn vs. CK group. However, “glutathione metabolism” and “ABC transporter” pathways were obviously enriched in high Zn vs. excess Zn conditions, implying their potential functions in alleviating the oxidative damage and Zn efflux caused by Zn toxicity. Together, this study identified key miRNAs that respond to both Zn deficiency and excess Zn in bread wheat, revealing distinct regulatory patterns of the target genes in different Zn supply conditions. These findings provide a new field and valuable candidate miRNAs for molecular breeding aimed at improving zinc’s utilization efficiency in wheat.
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Claims 12
Claim · model-written summary
High zinc promoted wheat plant growth.
- Taxon
- bread wheat (Triticum aestivum L.)
Triticum aestivume1 - Treatment
- high Zne0
- Outcome
- wheat plant growthe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high Zn promoted wheat plant growth”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Low zinc inhibited wheat plant growth.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- lowe0
- Outcome
- wheat plant growthe0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low and excess Zn resulted in wheat plant growth inhibition and oxidative stress”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Excess zinc inhibited wheat plant growth.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- excess Zne0
- Outcome
- wheat plant growthe0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low and excess Zn resulted in wheat plant growth inhibition and oxidative stress”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Low zinc produced oxidative stress in wheat.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- lowe0
- Outcome
- oxidative stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low and excess Zn resulted in wheat plant growth inhibition and oxidative stress”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Excess zinc produced oxidative stress in wheat.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- excess Zne0
- Outcome
- oxidative stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low and excess Zn resulted in wheat plant growth inhibition and oxidative stress”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Sequencing identified 798 wheat miRNAs, including 70 known and 728 novel ones.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Outcome
- miRNAse0
- Value
- 798e0
- Qualifier
- including 70 known and 728 novel miRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 798 miRNAs (including 70 known and 728 novel miRNAs) were identified”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Ten known and 122 novel miRNAs were differentially expressed.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Outcome
- miRNAs were differentially expressede0
- Value
- 10 known and 122 novele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10 known and 122 novel miRNAs were differentially expressed”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Several key miRNAs were up-regulated under low zinc.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- low Zne0
- Outcome
- miR397-5p, miR398, 4D_25791, and 5A_27668e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR397-5p, miR398, 4D_25791, and 5A_27668, are up-regulated under low Zn”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
The same key miRNAs were down-regulated under high zinc.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- high Zne0
- Outcome
- miR397-5p, miR398, 4D_25791, and 5A_27668e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR397-5p, miR398, 4D_25791, and 5A_27668, are up-regulated under low Zn but down-regulated under high Zn and excess Zn”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
The same key miRNAs were down-regulated under excess zinc.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Treatment
- excess Zne0
- Outcome
- miR397-5p, miR398, 4D_25791, and 5A_27668e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR397-5p, miR398, 4D_25791, and 5A_27668, are up-regulated under low Zn but down-regulated under high Zn and excess Zn”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Under low zinc versus control, predicted miRNA target genes focused on zinc ion and divalent cation transport and cellular detoxification.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Outcome
- regulated genes of these miRNAse0
- Qualifier
- low Zn vs. CK groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regulated genes of these miRNAs focused on “zinc ion transmembrane transporter activity”, “divalent inorganic cation transmembrane transporter activity”, and “cellular detoxification” processes in the low Zn vs. CK group”
e1“bread wheat (Triticum aestivum L.)”
Claim · model-written summary
Glutathione metabolism and ABC transporter pathways were enriched under high versus excess zinc.
- Taxon
- bread wheat (Triticum aestivum L.)Triticum aestivume1
- Outcome
- “glutathione metabolism” and “ABC transporter” pathwayse0
- Qualifier
- high Zn vs. excess Zn conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0““glutathione metabolism” and “ABC transporter” pathways were obviously enriched in high Zn vs. excess Zn conditions”
e1“bread wheat (Triticum aestivum L.)”
The same questions in other papers
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154 papers in the same direction as this paper · 43 in the opposite direction · 2 reporting no difference from an alternative
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See all 200 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.