Abstract
Fractionation is key for enriching bioactive compounds in traditional Chinese medicine, yet systematic studies on chemical differences across polarity-based fractions are limited. This study systematically compared the chemical profiles of four polarity fractions from a Gentiana scabra Bunge ethanol-water extract to elucidate the distribution patterns of its major active constituents. An integrated approach of untargeted LC-MS and quantitative HPLC was employed. The contents of gentiopicroside, isoorientin, and gentisin generally increased with ethanol concentration, with gentisin showing a more favorable elution trend. Notably, isoorientin and gentisin exhibited co-enrichment specifically in the 50% ethanol fraction (GSE50), resulting in its most complex and balanced chemical profile. This work provides the first systematic elucidation of these distribution patterns, establishing a foundation for precision preparation and quality control based on distinct chemical profiles.
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Claims 4
Claim · model-written summary
The contents of gentiopicroside, isoorientin and gentisin in Gentiana scabra extract fractions generally increased with ethanol concentration.
- Taxon
- Gentiana scabra Bunge
Gentiana scabrae0 - Treatment
- ethanol concentratione1
- Outcome
- contents of gentiopicroside, isoorientin, and gentisine1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four polarity fractions from a Gentiana scabra Bunge ethanol-water extract”
e1“The contents of gentiopicroside, isoorientin, and gentisin generally increased with ethanol concentration”
Claim · model-written summary
Gentisin showed a more favorable elution trend than the other major constituents across the fractions.
- Taxon
- Gentiana scabra BungeGentiana scabrae0
- Outcome
- gentisin showing a more favorable elution trende1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four polarity fractions from a Gentiana scabra Bunge ethanol-water extract”
e1“gentisin showing a more favorable elution trend”
Claim · model-written summary
Isoorientin and gentisin were co-enriched specifically in the 50% ethanol fraction (GSE50).
- Taxon
- Gentiana scabra BungeGentiana scabrae0
- Outcome
- isoorientin and gentisin exhibited co-enrichmente1
- Qualifier
- specifically in the 50% ethanol fraction (GSE50)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four polarity fractions from a Gentiana scabra Bunge ethanol-water extract”
e1“isoorientin and gentisin exhibited co-enrichment specifically in the 50% ethanol fraction (GSE50)”
Claim · model-written summary
The 50% ethanol fraction (GSE50) had the most complex and balanced chemical profile.
- Taxon
- Gentiana scabra BungeGentiana scabrae0
- Outcome
- most complex and balanced chemical profilee1
- Qualifier
- 50% ethanol fraction (GSE50)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four polarity fractions from a Gentiana scabra Bunge ethanol-water extract”
e1“co-enrichment specifically in the 50% ethanol fraction (GSE50), resulting in its most complex and balanced chemical profile”