Paper · 2026 · Plants

Comparison of Biochemical and Physiological Properties of Two Brassica Microgreens Cultivated in Two Growth Systems

Michele Ciriello; Christophe El-Nakhel; Giovanna Marta Fusco; et al.

8
claims
15
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by

Abstract

Microgreens are promising crops for low-input systems, but the roles of species traits and root environments under uncontrolled greenhouse conditions are not yet fully addressed. In this study, mibuna and pak choi were evaluated in the presence or absence of substrate to clarify how genetic and physical factors shape growth and biochemical composition. Clear species-dependent differences emerged. Pak choi showed higher constitutive levels of chlorophyll a, anthocyanins, and key osmotic ions, and these features contributed to a more hydrated tissue profile. Mibuna instead exhibited a higher dry matter content, approximately 7% compared with 5.86% in pak choi, and a lighter canopy. The use of peat markedly improved overall growth. Fresh yield increased by more than 50%, and dry yield increased by almost 48% compared with the substrate-free system. Peat also enhanced organic acid metabolism, with malate and citrate increasing by 277 and 102%, respectively. Despite such differences, nitrate concentrations remained low and within safe limits for leafy vegetables in all treatments. The results indicate that species identity and the cultivation system significantly shaped overall microgreen performance under low-input conditions. Understanding these drivers provides a foundation for optimizing production while maintaining desirable nutritional traits.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Pak choi showed higher constitutive levels of chlorophyll a, anthocyanins and key osmotic ions than mibuna.

Taxon
Pak choie1
Outcome
constitutive levels of chlorophyll a, anthocyanins, and key osmotic ionse1
Direction
highere1

Verbatim

e1

“Pak choi showed higher constitutive levels of chlorophyll a, anthocyanins, and key osmotic ions”

✓ located verbatim in the source · abstract, characters 400–495 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Mibuna had a higher dry matter content, approximately 7%, compared with 5.86% in pak choi.

Taxon
Mibunae1
Outcome
dry matter contente1
Direction
highere1
Value
approximately 7%e1
Qualifier
5.86%e1
Comparator
pak choie1

Verbatim

e1

“Mibuna instead exhibited a higher dry matter content, approximately 7% compared with 5.86% in pak choi”

✓ located verbatim in the source · abstract, characters 563–665 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Using peat increased fresh yield of Brassica microgreens by more than 50% compared with the substrate-free system.

Taxon
Microgreenst0
Treatment
use of peate1
Outcome
Fresh yielde2
Direction
increasede2
Value
more than 50%e2
Comparator
substrate-free systeme2

Verbatim

t0

“Brassica Microgreens”

✓ located verbatim in the source · title, characters 62–82 · show in text ↑
e1

“The use of peat markedly improved overall growth”

✓ located verbatim in the source · abstract, characters 689–737 · show in text ↑
e2

“Fresh yield increased by more than 50%, and dry yield increased by almost 48% compared with the substrate-free system”

✓ located verbatim in the source · abstract, characters 739–856 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Using peat increased dry yield of Brassica microgreens by almost 48% compared with the substrate-free system.

Taxon
Microgreenst0
Treatment
use of peate1
Outcome
dry yielde2
Direction
increasede2
Value
almost 48%e2
Comparator
substrate-free systeme2

Verbatim

t0

“Brassica Microgreens”

✓ located verbatim in the source · title, characters 62–82 · show in text ↑
e1

“The use of peat markedly improved overall growth”

✓ located verbatim in the source · abstract, characters 689–737 · show in text ↑
e2

“Fresh yield increased by more than 50%, and dry yield increased by almost 48% compared with the substrate-free system”

✓ located verbatim in the source · abstract, characters 739–856 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Peat increased malate in Brassica microgreens by 277%.

Taxon
Microgreenst0
Treatment
Peate1
Outcome
malatee1
Direction
increasinge1
Value
277e1

Not reportedComparator

Verbatim

t0

“Brassica Microgreens”

✓ located verbatim in the source · title, characters 62–82 · show in text ↑
e1

“Peat also enhanced organic acid metabolism, with malate and citrate increasing by 277 and 102%, respectively”

✓ located verbatim in the source · abstract, characters 858–966 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Peat increased citrate in Brassica microgreens by 102%.

Taxon
Microgreenst0
Treatment
Peate1
Outcome
citratee1
Direction
increasinge1
Value
102%e1

Not reportedComparator

Verbatim

t0

“Brassica Microgreens”

✓ located verbatim in the source · title, characters 62–82 · show in text ↑
e1

“Peat also enhanced organic acid metabolism, with malate and citrate increasing by 277 and 102%, respectively”

✓ located verbatim in the source · abstract, characters 858–966 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Nitrate concentrations in the microgreens remained low and within safe limits for leafy vegetables in all treatments.

Taxon
Microgreenst0
Outcome
nitrate concentrationse1

Not reportedDirection

Verbatim

t0

“Brassica Microgreens”

✓ located verbatim in the source · title, characters 62–82 · show in text ↑
e1

“nitrate concentrations remained low and within safe limits for leafy vegetables in all treatments”

✓ located verbatim in the source · abstract, characters 994–1091 · show in text ↑
Associationauthor interpretationoriginal resultsignificant (as stated)measured

Claim · model-written summary

Species identity and the cultivation system significantly shaped overall microgreen performance under low-input conditions.

Taxon
microgreene1
Treatment
cultivation systeme1
Outcome
overall microgreen performancee1

Not reportedDirection

Verbatim

e1

“species identity and the cultivation system significantly shaped overall microgreen performance under low-input conditions”

✓ located verbatim in the source · abstract, characters 1119–1241 · show in text ↑